Mechanisms of miR-18a-5p Target NEDD9-Mediated Suppression of H5N1 Influenza Virus in Mammalian and Avian Hosts

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(3), P. 240 - 240

Published: March 3, 2025

MicroRNAs (miRNAs) are key regulators of gene expression, exerting post-translational control through mRNA silencing or degradation. These molecules play pivotal roles in host–pathogen interactions, particularly modulating antiviral immune responses. The global public health threat posed by the H5N1 Highly Pathogenic Avian Influenza (HPAI) virus necessitates urgent exploration novel therapeutic strategies. Our investigation revealed significant dysregulation miR-18a-5p following influenza infection, observed consistently across both vitro and vivo models. Experimental evidence demonstrated that overexpression effectively inhibits propagation multiple mechanisms: (1) studies using A549 cells transfected with mimics showed a substantial reduction viral replication; (2) animal models (mice chickens) elevated expression exhibited markedly suppressed AIV replication, reduced pathogenicity, improved survival rates. potential was evident its ability to significantly decrease mortality rates H5N1-infected animals. Furthermore, this miRNA robust protective effects against virus-induced lung damage, suggesting dual role preventing treating infections. findings position as promising candidate for development broad-spectrum interventions, offering strategic approach combat serious challenge.

Language: Английский

Lung dECM microcarriers for 3D proliferation of hUCMSCs and expression of growth factors

Journal of the American Ceramic Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 9, 2025

Abstract A simple 3D proliferation strategy for human umbilical cord‐derived mesenchymal stem cells (hUCMSCs) was developed based on porcine decellularized lung tissue microcarriers (DLTMs). The fixed with 75% ethanol, followed by decellularization and digestion pepsin‐acetic acid solution to form DLTMs an average diameter of 5–40 µm. presence key proteins, including collagen I, IV, laminin, fibronectin, elastin, confirmed through immunofluorescence staining, while the amount polysaccharides quantified. After centrifugation, hUCMSCs suspension formed spheroids which were cultured 14 days. Various techniques, DAPI, FDA H&E DNA quantification used assess performance hUCMSCs. Flow cytometric immunophenotyping employed, gene protein expressions cytokines growth factors such as IL‐10, HGF, KGF, Ang‐1 evaluated. results demonstrated that exhibited a porous structure simulated in vivo microenvironment, effectively enhancing immunomodulatory functions This showed potential integration bioreactor systems large‐scale production hUCMSC‐derived paracrine products therapeutic applications.

Language: Английский

Withaferin-A induced Vimentin S56 phosphorylation dissociates NEDD9 signaling loop to regress progressive metastatic melanoma into lung adenocarcinoma.

Chemico-Biological Interactions, Journal Year: 2024, Volume and Issue: unknown, P. 111319 - 111319

Published: Nov. 1, 2024

Language: Английский