Active prokaryotic and eukaryotic viral ecology across spatial scale in a deep-sea brine pool DOI Creative Commons
Benjamin Minch,

Morgan Chakraborty,

Sam J. Purkis

et al.

ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 1, 2024

Abstract Deep-sea brine pools represent rare, extreme environments, providing unique insight into the limits of life on Earth, and by analogy, plausibility beyond it. A distinguishing feature many is presence thick microbial mats that develop at brine–seawater interface. While these bacterial archaeal communities have received moderate attention, viruses their host interactions in environments remain underexplored. To bridge this knowledge gap, we leveraged metagenomic metatranscriptomic data from three distinct zones within NEOM pool system (Gulf Aqaba) to reveal active viral ecology around pools. We report a remarkable diversity activity infecting hosts environment, including giant viruses, RNA jumbo phages, Polinton-like viruses. Many form clades—suggesting untapped ecosystem. Brine exhibit zone-specific differences infection strategy—with lysogeny dominating mat further away pool’s center. linked metabolically important prokaryotes—including association between phage key manganese-oxidizing arsenic-metabolizing bacterium. These foundational results illuminate role modulating biogeochemistry through revealing novel diversity, associations, spatial heterogeneity dynamics.

Language: Английский

Cryptic infection of a giant virus in a unicellular green alga DOI
Maria P. Erazo-Garcia, Uri Sheyn, Zachary K. Barth

et al.

Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Latency is a common strategy in wide range of viral lineages, but its prevalence giant viruses remains unknown. Here we describe 617 kbp integrated element the model green alga Chlamydomonas reinhardtii . We resolve genome using long-read sequencing, identify putative polinton-like integrase, and show that particles accumulate primarily during stationary growth phase. A diverse array viral-encoded selfish genetic elements expressed activity, including several Fanzor nuclease-encoding transposable elements. In addition, field isolates sp. harbor signatures endogenous related to C. virus exhibit similar infection dynamics, suggesting latency prevalent natural host communities. Our work describes an unusually large temperate unicellular eukaryote, substantially expanding scope cryptic infections virosphere.

Language: Английский

Citations

0
BEREN: A bioinformatic tool for recovering Giant viruses, Polinton-like Viruses, and Virophages in metagenomic data DOI Creative Commons
Benjamin Minch, Mohammad Moniruzzaman

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 9, 2024

Abstract Viruses in the kingdom Bamfordvirae , specifically giant viruses (NCLDVs) phylum Nucleocytoviricota and smaller members Preplasmiviricota phylum, are widespread important groups of that infect eukaryotes. While this such as viruses, polinton-like virophages have gained large interest from researchers recent years, there is still a lack streamlined tools for recovery their genomes metagenomic datasets. Here, we present BEREN, comprehensive bioinformatic tool to unlock diversity these metagenomes through five modules NCLDV genome, contig, marker gene recovery, metabolic protein annotation, genome identification annotation. BEREN’s performance was benchmarked against other mainstream virus using mock metagenome, demonstrating superior rates contigs genomes. Applied real-world dataset Baltic Sea, BEREN identified diverse members, giving insight into viral interactions functions region. Overall, offers user-friendly, transparent solution studying ecological functional roles eukaryotic facilitating broader access analysis.

Language: Английский

Citations

1
Active prokaryotic and eukaryotic viral ecology across spatial scale in a deep-sea brine pool DOI Creative Commons
Benjamin Minch,

Morgan Chakraborty,

Sam J. Purkis

et al.

ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 1, 2024

Abstract Deep-sea brine pools represent rare, extreme environments, providing unique insight into the limits of life on Earth, and by analogy, plausibility beyond it. A distinguishing feature many is presence thick microbial mats that develop at brine–seawater interface. While these bacterial archaeal communities have received moderate attention, viruses their host interactions in environments remain underexplored. To bridge this knowledge gap, we leveraged metagenomic metatranscriptomic data from three distinct zones within NEOM pool system (Gulf Aqaba) to reveal active viral ecology around pools. We report a remarkable diversity activity infecting hosts environment, including giant viruses, RNA jumbo phages, Polinton-like viruses. Many form clades—suggesting untapped ecosystem. Brine exhibit zone-specific differences infection strategy—with lysogeny dominating mat further away pool’s center. linked metabolically important prokaryotes—including association between phage key manganese-oxidizing arsenic-metabolizing bacterium. These foundational results illuminate role modulating biogeochemistry through revealing novel diversity, associations, spatial heterogeneity dynamics.

Language: Английский

Citations

0