Microbial interactions within beneficial consortia promote soil health

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 900, P. 165801 - 165801

Published: July 25, 2023

Language: Английский

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“What I cannot create, I do not understand": elucidating microbe–microbe interactions to facilitate plant microbiome engineering

Current Opinion in Microbiology, Journal Year: 2023, Volume and Issue: 72, P. 102283 - 102283

Published: March 1, 2023

Language: Английский

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The dawn of the revolution that will allow us to precisely describe how microbiomes function

Journal of Proteomics, Journal Year: 2025, Volume and Issue: 316, P. 105430 - 105430

Published: March 11, 2025

The community of microorganisms inhabiting a specific environment, such as the human gut - including bacteria, fungi, archaea, viruses, protozoa, and others is known microbiota. A holobiont, in turn, refers to an integrated ecological unit where microbial communities function interact with their host, thus more integrative concept. To understand processes involved, diversity present must be identified molecular components quantified, especially proteins. Indeed, proteins through roles catalytic units, structural components, signaling molecules are main drivers biological processes. Metagenomics has significantly expanded what we know about genetic material microbiota, revealing functional potential; metabolomics delivers overall snapshot metabolites produced by community. But metaproteomics offers complementary approach explore microbiome holobiont functionality focusing on active pathways from each taxon. Significant recent advances high-resolution tandem mass spectrometry have greatly catalog peptide sequences accessible sample, creating conditions for unprecedented taxonomical profiling, while also providing accurate biomass quantification, detailed protein characterization, greater capacity monitor abundance distinguish host biomarkers. By integrating artificial intelligence into pipeline, extended datasets can now efficiently mined gain comprehensive view complex systems, paving way next-generation metaproteomics. In this perspective, I discuss transformative potential methodology. We cusp remarkable omic revolution that promises uncover intricate workings microbiomes producing vast array new knowledge multiple applications. SIGNIFICANCE: Metaproteomics provides powerful lens investigate identifying quantifying within Recent breakthroughs dramatically repertoire detectable per sample. This progress enables taxonomic resolution identification, precise monitoring, unique identification commentary, delve distinctive features make tool. advancements argue primary challenge analyzing samples shifting data acquisition interpretation. With integration intelligence, believe poised become next Big Thing research, unlocking profound insights ecosystem dynamics.

Language: Английский

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Species‐Specific Epigenetic Signature Associates With Heat Stress Tolerance in the Perennial Tree Species Populus

GCB Bioenergy, Journal Year: 2025, Volume and Issue: 17(5)

Published: April 26, 2025

ABSTRACT Epigenetic regulation in annual plants is recognized as a key component of recurring stress acclimation and adaptation, but reports on perennial tree species are limited. In this study, two contrasting species, Populus trichocarpa deltoides , an F1 hybrid cross between them showed species‐specific epigenetic physiological responses to heat (42°C) following priming (35°C). By analyzing whole‐genome methylation, transcriptomics, proteomics, metabolomics, photosynthesis parameters, we found that P. expresses specific signatures response heat, resulting improved photosynthetic efficiency compared . Conversely, displayed signaling defense mechanisms could not sustain net assimilation rate despite maintaining higher gas exchange. Heat increased transcript levels thermotolerance‐related transcription factors, such SPL12. Selected regions the promoter SPL12 differential methylation direct followed by stress. As result, upregulation downstream genes associated increases protein metabolite abundance for adaptation were exhibited. Consequently, enhanced exchange rates, trait lacking These results imply may be universally effective enhancing plant performance under stress, particularly species. However, can acclimate withstand elevated temperature better. Our study has demonstrated priming‐based attained through crossbreeding, indicating its potential use breeding programs.

Language: Английский

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The Promises, Challenges, and Opportunities of Omics for Studying the Plant Holobiont

Microorganisms, Journal Year: 2022, Volume and Issue: 10(10), P. 2013 - 2013

Published: Oct. 12, 2022

Microorganisms are critical drivers of biological processes that contribute significantly to plant sustainability and productivity. In recent years, emerging research on holobiont theory microbial invasion ecology has radically transformed how we study plant-microbe interactions. Over the last few have witnessed an accelerating pace advancements breadth questions answered using omic technologies. Herein, discuss current state-of-the-art genomics, transcriptomics, proteomics, metabolomics techniques reliably transcend task studying interactions while acknowledging existing limitations impeding our understanding holobionts.

Language: Английский

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