Proteolytic processing of both RXLR and EER motifs in oomycete effectors DOI Creative Commons
Lin Xu, Shumei Wang, Wei Wang

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Summary Arg‐any amino acid‐Leu‐Arg (RXLR) effectors are central oomycete virulence factors that suppress plant immunity. Relatively little is known about how they processed post‐translationally before delivery into host cells. A range of molecular, cell and biochemical processes were used to investigate proteolytic processing RXLR Glu‐Glu‐Arg (EER) motifs in Phytophthora infestans effectors. Proteolytic cleavage at the motif occurred secretion all tested, suggesting it a general rule. Cleavage between leucine second arginine. There was no naturally occurring structured region Pi21388/AvrBlb1, or one introduced similar position effector Pi04314, keeping with being positionally constrained, potentially disordered regions closely following signal peptide. Remarkably, independent EER motif, often found immediately after RXLR, also observed, Full‐length expressed Nicotiana benthamiana revealed that, although secreted, poorly processed, does not occur eukaryotic We conclude whether possessing both EER, either alone, these likely generally proteolytically secretion.

Language: Английский

Focus on the Effectors at the Interface of Plant–Microbe Interactions DOI Creative Commons
Melania Figueroa, Gitta Coaker, K. Kanyuka

et al.

Molecular Plant-Microbe Interactions, Journal Year: 2024, Volume and Issue: 37(3), P. 168 - 170

Published: March 1, 2024

Language: Английский

Citations

1
Proteolytic processing of both RXLR and EER motifs in oomycete effectors DOI Creative Commons
Lin Xu, Shumei Wang, Wei Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 19, 2024

Abstract Arg-any amino acid-Leu-Arg (RXLR) effectors are central oomycete virulence factors that target diverse host proteins and processes to suppress plant immunity. Relatively little is known about how they processed post-translationally before delivery into cells. Proteolytic cleavage at the RXLR motif was observed occur prior secretion in all Phytophthora infestans tested, suggesting it a general rule, between leucine second arginine. There no of naturally occurring structured region Pi21388/AvrBlb1, or one introduced similar position effector Pi04314, keeping with being positionally constrained, potentially disordered regions closely following signal peptide. Remarkably, independent proteolytic Glu-Glu-Arg (EER) motif, often found immediately downstream RXLR, also effectors, after Expression full-length Nicotiana benthamiana revealed that, although secreted, were poorly processed, EER does not eukaryotic Our observations indicate whether possessing both EER, either alone, these likely proteolytically cases.

Language: Английский

Citations

1
Proteolytic processing of both RXLR and EER motifs in oomycete effectors DOI Creative Commons
Lin Xu, Shumei Wang, Wei Wang

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Summary Arg‐any amino acid‐Leu‐Arg (RXLR) effectors are central oomycete virulence factors that suppress plant immunity. Relatively little is known about how they processed post‐translationally before delivery into host cells. A range of molecular, cell and biochemical processes were used to investigate proteolytic processing RXLR Glu‐Glu‐Arg (EER) motifs in Phytophthora infestans effectors. Proteolytic cleavage at the motif occurred secretion all tested, suggesting it a general rule. Cleavage between leucine second arginine. There was no naturally occurring structured region Pi21388/AvrBlb1, or one introduced similar position effector Pi04314, keeping with being positionally constrained, potentially disordered regions closely following signal peptide. Remarkably, independent EER motif, often found immediately after RXLR, also observed, Full‐length expressed Nicotiana benthamiana revealed that, although secreted, poorly processed, does not occur eukaryotic We conclude whether possessing both EER, either alone, these likely generally proteolytically secretion.

Language: Английский

Citations

1