Development of an Effective Sterilization Protocol for Plant Tissue Culture Studies in Superfruit Aronia [Aronia melanocarpa (Michaux) Elliot] DOI
Çağlar Kaya, Şehnaz Özatay

Black Sea Journal of Agriculture, Journal Year: 2024, Volume and Issue: 7(6), P. 679 - 685

Published: Oct. 15, 2024

Effective sterilization protocols are crucial for a successful tissue culture study in Aronia. These directly influence contamination rates, shoot health, and root development. In this context, the aims is to develop an effective protocol plant studies Aronia [Aronia melanocarpa (Michaux) Elliot], commonly known as "superfruit." study, Nero variety tips were used material. The sterilized transferred respective media randomized parcels trial pattern with three replicates, each containing explants per replicate. Various concentrations combinations of sterilizing agents, such sodium hypochlorite (NaOCl), hydrogen peroxide (H2O2), mercuric chloride (HgCl2), ethanol (C2H5OH), evaluated determine their effectiveness maintaining health reducing contamination. Twelve developed, incorporating different these chemicals. data subjected statistical analysis using SAS software package (LSD0.05-Proc GLM). Analysis variance (ANOVA) was performed evaluate differences among treatments parameter. results showed that combination 5% NaOCl 3% H2O2 (10 min each) provided lowest average rate 0.0%, highest number uncontaminated 9.0 pieces, length 3.0 cm 2.5 demonstrating efficiency combination. On other hand, HgCl2, especially at higher concentrations, resulted impaired High also contributed sterilization, 7% min) 80% (5 yielding low (22.0%) preserving health. This emphasizes balancing between control viability. findings expected benefit improvement development techniques similar species, providing basis further research on practices, currently limited culture.

Language: Английский

Developing epidemiological preparedness for a plant disease invasion: Modelling citrus huánglóngbìng in the European Union DOI Creative Commons
John Ellis, Elena Lázaro, Beatriz Duarte

et al.

Plants People Planet, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

Societal Impact Statement Huánglóngbìng (HLB) is a bacterial disease of citrus that has significantly impacted Brazil and the United States, although production in Mediterranean Basin remains unaffected. By developing mathematical model spread Spain, we tested surveillance control strategies before any future HLB entry EU. We found while some might be maintained by roguing, this requires extensive significant chemical control, perhaps also including testing psyllids (which pathogen) for DNA. Our work highlights key importance early detection (including asymptomatic infection) vector management. Summary (HLB; greening) most damaging worldwide. While States have been affected decades, not reported European Union (EU). However, vector, African psyllid, already Portugal Spain. In 2023, major Asian was first Cyprus. develop landscape‐scale, epidemiological model, accounting heterogeneous cultivation dispersal, as well climate use our to predict dynamics an epidemic vectored psyllid high‐density areas assessing strategies. Without management, large infected within 10–20 years. Even with visual surveillance, will widespread on detection, making eradication unlikely. Nevertheless, increased inspection roguing following particularly if coupled intensive insecticide use, could sustain citriculture decade or more, albeit reduced production. effective may require application rates and/or active substances no longer authorised Strategies targeting infection more successful. Detection bacteriliferous vectors—sometimes possible long plants show symptoms—could reduce lags management commences. If HLB‐positive vectors were followed sprays, greatly improve outcomes. modelling component preparedness pathogen invasion is, at least somewhat, predictable advance.

Language: Английский

Citations

0
Design, Synthesis, and Anti-Oomycete and Antifungal Activities of Novel Paeonol Derivatives Containing a Pyrazole Ring DOI
Yibo Liu, Yuee Tian, Xiaozhong Peng

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Paeonol is the main active ingredient in Chinese medicinal herb peony root bark. To discover biorational natural product-based fungicides, 25 paeonol derivatives (5a-y) containing a pyrazole ring were designed and synthesized by ingeniously introducing five-membered heterocyclic ring, their structures well characterized 1H NMR, 13C high-resolution mass spectrometer (HRMS), m.p. The stereochemical configurations of six target compounds, 5c, 5f, 5g, 5o, 5s, 5v, unambiguously subjected to single-crystal X-ray diffraction. Furthermore, we evaluated bioactivities these compounds as anti-oomycete antifungal agents against two serious agricultural diseases, Phytophthora capsici Fusarium graminearum. Among all tested (1) nine 5b-d, 5r, 5u, 5w displayed promising P. capsici, with EC50 values ranging from 22.1 34.5 mg/L, protective effect vivo further confirmed above results. (2) Six 5k, 5m, 5t, 5w, 5x F. graminearum, 38.2 86.0 mg/L. Moreover, some interesting results structure-activity relationships also observed. this study pave way for design, synthesis, development potential botanical crop protection.

Language: Английский

Citations

0
Rapid population flux in bacterial spot xanthomonads during a transition in dominance between two genotypes in consecutive tomato production seasons and identification of a new speciesXanthomonas oklahomensissp. nov DOI Creative Commons

Brett Johnson,

Aastha Subedi, J. P. Damicone

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract In the bacterial spot of tomato disease complex, Xanthomonas euvesicatoria pv. perforans ( Xep ) is known to outcompete X. Xee ). The result, over time in production systems where both pathogens are present, anticipated displacement by . this study, we characterized population recovered strains from grower fields response a outbreak east and central Oklahoma 2018 2019. Tomato pepper sampled earlier years were included provide additional context. Phenotypic genome-based analyses showed marked differences race species composition strains. All pre-2018 (2001-2014) T3, except one T4 strain. consisted T1 while 2019 same locations exclusively form cluster with 2014 strain on phylogenetic tree, suggesting population. Only an expanded host range pepper. also variable copper sensitivity effector content. We additionally non-bacterial xanthomonads, which belongs new species, oklahomensis sp. nov. These results contributes novel insights understanding genomic heterogeneity seasonal flux between competing genotypes during outbreaks can be considered when developing management strategies.

Language: Английский

Citations

0
The environmental and anthropogenic impacts of nanoparticles on forest trees DOI

Mehrdad Alizadeh,

Elshan Musazade, Sirvan Qaderi

et al.

Journal of Nanoparticle Research, Journal Year: 2025, Volume and Issue: 27(6)

Published: May 19, 2025

Language: Английский

Citations

0
Synthesis, Anti‐oomycete and Anti‐fungal Activities of Novel Eugenol/Dihydroeugenol Derivatives Bearing Carbamate Moiety DOI

Huilu Sun,

Ruxue Wei,

Gen‐Qiang Chen

et al.

Chemistry & Biodiversity, Journal Year: 2025, Volume and Issue: unknown

Published: May 30, 2025

ABSTRACT Eugenol is the main component of clove basil oil extracted from plants such as Syzygium aromaticum and Ocimum gratissimum . To discover biorational natural product‐based pesticides, a series eugenol/dihydroeugenol derivatives containing carbamate moiety were prepared by introducing an active aminooyloxy group at its C1 hydroxy position, their structures well characterized proton nuclear magnetic resonance ( 1 H NMR), carbon‐13 NMR 13 C high‐resolution mass spectrometry, melting point. Bioactivities these target compounds anti‐oomycete anti‐fungal agents against two serious agricultural pests, Phytophthora capsici Fusarium graminearum evaluated. Among assessed compounds: (1) Title 1b 2b showed significant F. , with half maximal effective concentration values 29.13 35.04 mg/L, respectively. (2) The title have obvious selectivity, activity significantly better than activity. (3) activities aminoalkyloyloxy hydroxyl position eugenol or dihydroeugenol are that aminoaryloyloxy group. (4) presence double bond beneficial for increasing activity, reduction

Language: Английский

Citations

0
Developing epidemiological preparedness for a plant disease invasion: modelling citrus huanglongbingin the European Union DOI Creative Commons
John Ellis, Elena Lázaro, Beatriz Duarte

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 6, 2024

Abstract Huánglóngbíng (HLB; citrus greening) is the most damaging disease of worldwide. While production in USA and Brazil have been affected for decades, HLB has not detected European Union (EU). However, psyllid vectors already invaded spread Portugal Spain, 2023 species known to vector Americas was first reported within EU. We develop a landscape-scale, epidemiological model, accounting heterogeneous cultivation dispersal, as well climate management. use our model predict dynamics following introduction into high-density areas assessing detection control strategies. Even with significant visual surveillance, we any epidemic will be widespread on detection, eradication unlikely. Introducing increased inspection roguing particularly if coupled intensive insecticide use, could potentially sustain some time. this may require chemical application rates that are permissible Disease management strategies targeting asymptomatic infection likely lead more successful outcomes. Our work highlights modelling key component developing preparedness pathogen invasion is, at least somewhat, predictable advance.

Language: Английский

Citations

1
Development of an Effective Sterilization Protocol for Plant Tissue Culture Studies in Superfruit Aronia [Aronia melanocarpa (Michaux) Elliot] DOI
Çağlar Kaya, Şehnaz Özatay

Black Sea Journal of Agriculture, Journal Year: 2024, Volume and Issue: 7(6), P. 679 - 685

Published: Oct. 15, 2024

Effective sterilization protocols are crucial for a successful tissue culture study in Aronia. These directly influence contamination rates, shoot health, and root development. In this context, the aims is to develop an effective protocol plant studies Aronia [Aronia melanocarpa (Michaux) Elliot], commonly known as "superfruit." study, Nero variety tips were used material. The sterilized transferred respective media randomized parcels trial pattern with three replicates, each containing explants per replicate. Various concentrations combinations of sterilizing agents, such sodium hypochlorite (NaOCl), hydrogen peroxide (H2O2), mercuric chloride (HgCl2), ethanol (C2H5OH), evaluated determine their effectiveness maintaining health reducing contamination. Twelve developed, incorporating different these chemicals. data subjected statistical analysis using SAS software package (LSD0.05-Proc GLM). Analysis variance (ANOVA) was performed evaluate differences among treatments parameter. results showed that combination 5% NaOCl 3% H2O2 (10 min each) provided lowest average rate 0.0%, highest number uncontaminated 9.0 pieces, length 3.0 cm 2.5 demonstrating efficiency combination. On other hand, HgCl2, especially at higher concentrations, resulted impaired High also contributed sterilization, 7% min) 80% (5 yielding low (22.0%) preserving health. This emphasizes balancing between control viability. findings expected benefit improvement development techniques similar species, providing basis further research on practices, currently limited culture.

Language: Английский

Citations

0