Functional Characterization of Transcriptional Regulator Rem in Candidatus Liberibacter asiaticus DOI
Wenting Wang, Jin Xu, Nian Wang

et al.

Phytopathology, Journal Year: 2025, Volume and Issue: 115(5), P. 454 - 468

Published: Feb. 1, 2025

Citrus Huanglongbing, caused by 'Candidatus Liberibacter asiaticus' (CLas), is the most devastating citrus disease worldwide. The CLas genome much smaller than those of its relatives, such as Sinorhizobium meliloti, due to reductive evolution. Because has not been cultured in artificial media, despite some progress co-cultivating, and because genetic manipulation remains impossible, understanding biology very limited. Usually, 10% total genes bacteria are regulatory genes, but only 2% encode transcriptional factors. Here, 20 regulators were predicted, including nine (lsrB, ldtR, rem, visR, visN, ctrA, mucR, pelD, atoC) directly or indirectly involved regulating motility, five (rpoH, prbP, phrR, rirA, lsrB) oxidative stress response. We demonstrated that lsrB, visNR can complement corresponding mutants S. meliloti their reduced motility. further investigated traits controlled Rem using RNA sequencing analyses rem mutant versus complementation strains with remSmc remLas. Transcriptomic analysis showed RemLas significantly regulates expression which was used infer regulation identification homologous genes. found chemotaxis, transporters, phosphorylation flagellar transporter CLas. Among 39 putative RemLas-regulated CLas, 16 contain Rem-binding motif, 10 assembly. Taken together, this study offers valuable insights regarding many them motility unravels critical information infection hosts.

Language: Английский

Key Challenges in Plant Pathology in the Next Decade DOI
Nian Wang, George W. Sundin, Leonardo De La Fuente

et al.

Phytopathology, Journal Year: 2024, Volume and Issue: 114(5), P. 837 - 842

Published: May 1, 2024

Plant diseases significantly impact food security and safety. It was estimated that production needs to increase by 50% feed the projected 9.3 billion people 2050. Yet, plant pathogens pests are documented cause up 40% yield losses in major crops, including maize, rice, wheat, resulting annual worldwide economic of approximately US$220 billion. Yield due be 21.5% (10.1 28.1%) 30.3% (24.6 40.9%) 22.6% (19.5 41.4%) maize. In March 2023, The American Phytopathological Society (APS) conducted a survey identify rank key challenges pathology next decade. Phytopathology subsequently invited papers address those pathology, these were published as special issue. identified include climate change effect on disease triangle outbreaks, resistance mechanisms its applications, specific caused Candidatus Liberibacter spp. Xylella fastidiosa. Additionally, detection, natural man-made disasters, control strategies explored issue articles. Finally, aspects open access how publish articles maximize Findability, Accessibility, Interoperability, Reuse digital assets described. Only identifying tracking progress developing solutions for them will we able resolve issues ultimately ensure health, security,

Language: Английский

Citations

8
Functional Characterization of Transcriptional Regulator Rem in Candidatus Liberibacter asiaticus DOI
Wenting Wang, Jin Xu, Nian Wang

et al.

Phytopathology, Journal Year: 2025, Volume and Issue: 115(5), P. 454 - 468

Published: Feb. 1, 2025

Citrus Huanglongbing, caused by 'Candidatus Liberibacter asiaticus' (CLas), is the most devastating citrus disease worldwide. The CLas genome much smaller than those of its relatives, such as Sinorhizobium meliloti, due to reductive evolution. Because has not been cultured in artificial media, despite some progress co-cultivating, and because genetic manipulation remains impossible, understanding biology very limited. Usually, 10% total genes bacteria are regulatory genes, but only 2% encode transcriptional factors. Here, 20 regulators were predicted, including nine (lsrB, ldtR, rem, visR, visN, ctrA, mucR, pelD, atoC) directly or indirectly involved regulating motility, five (rpoH, prbP, phrR, rirA, lsrB) oxidative stress response. We demonstrated that lsrB, visNR can complement corresponding mutants S. meliloti their reduced motility. further investigated traits controlled Rem using RNA sequencing analyses rem mutant versus complementation strains with remSmc remLas. Transcriptomic analysis showed RemLas significantly regulates expression which was used infer regulation identification homologous genes. found chemotaxis, transporters, phosphorylation flagellar transporter CLas. Among 39 putative RemLas-regulated CLas, 16 contain Rem-binding motif, 10 assembly. Taken together, this study offers valuable insights regarding many them motility unravels critical information infection hosts.

Language: Английский

Citations

0