Published: Dec. 10, 2024
Language: Английский
Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16
Published: Feb. 20, 2025
The RNA-based spray-induced gene silencing (SIGS) technology represents an ecologically sustainable approach to crop protection and pathogen management. Following the recent approval of Ledprona as first sprayable double-stranded RNA (dsRNA) biopesticide by EPA at end 2023, SIGS has emerged a focal point in both academic industrial sectors. This review analyzes advances emerging trends SIGS. application for protection, including control insects, fungal pathogens, viruses, is briefly summarized. Distinguishing this from others, we delve into practical aspects technology, such selection screening target genes, large-scale production methods, delivery systems, highlighting major advancements these areas also addressing remaining questions issues, particularly concerning safety concerns controlling harmful weeds. Finally, emphasizes its integration with nanotechnology other methodologies. Collectively, rapid progress studies poised accelerate maturation technology.
Language: Английский
Citations
2
Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 6, 2025
RNA interference (RNAi)-based control technologies are gaining popularity as potential alternatives to synthetic fungicides in the ongoing effort manage plant pathogenic fungi. Among these methods, spray-induced gene silencing (SIGS) emerges particularly promising due its convenience and feasibility for development. This approach is a new technology disease management, which double-stranded RNAs (dsRNAs) targeting essential or virulence genes applied plants products subsequently absorbed by pathogens, triggering effect inhibition of infection process. Spray-induced has demonstrated efficacy laboratory settings against various fungal pathogens. However, research progressed from greenhouse field environments, novel challenges arose, such ensuring stability dsRNAs their effective delivery targets. Here, we provide practical guide SIGS outlines steps considerations needed designing assessing dsRNA molecules. It also addresses key inherent SIGS, including molecules, how nanoencapsulation can aid overcoming obstacles. Additionally, underscores existing knowledge gaps that warrant further aims assistance researchers, especially those field, encouraging advancement broad range
Language: Английский
Citations
1
Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown
Published: March 13, 2025
ABSTRACT RNA interference (RNAi) is increasingly used for plant protection against pathogens and pests. However, the traditional delivery method causes tissue damage, affected by environmental factors, faces difficulties in penetrating barriers of cell walls limitations species, ultimately leading to low efficiency. With advances nanotechnology, nanomaterials (NMs) have been identified as effective carriers nucleic acid because their ability operate independently external mechanical forces, prevent degradation bioenzymes, exhibit good biocompatibility, offer high loading capacity. This review summarizes application NM‐mediated RNAi pests, focusing on how different NMs break through plants, pathogens, pests according size, morphology, charge characteristics. Furthermore, we discuss advantages improvement strategies carriers, alongside assessing potential management
Language: Английский
Citations
0
Journal of Plant Sciences, Journal Year: 2025, Volume and Issue: 13(2), P. 38 - 58
Published: April 10, 2025
The global agricultural sector faces unprecedented challenges in meeting the projected food demand of 9.7 billion people by 2050, exacerbated adverse impacts climate change, such as increased droughts and temperature extremes. Nanobiotechnology, synergistic integration nanotechnology biotechnology, offers transformative solutions plant genetic engineering to enhance sustainability ensure security. Nanobiotechnology exploits unique physicochemical properties nanomaterials, enabling precise delivery materials, advanced gene editing, real-time monitoring cellular processes. Innovative nanoparticle-mediated methods facilitate transfer nucleic acids, proteins, other biomolecules into cells, overcoming limitations conventional transformation Agrobacterium-mediated gun technologies. For example, magnetic nanoparticles carbon nanotubes have shown promise genotype-independent material efficient transgene expression. This review highlights groundbreaking applications nanobiotechnology, including enhanced CRISPR/Cas9 components for accurate nanoscale sensors intracellular process monitoring, use mesoporous silica stable silencing. Despite these advancements, barriers nanoparticle biocompatibility, potential toxicity, scalability systems must be addressed. Regulatory frameworks ensuring safe adoption nanomaterials practices are equally critical. holds revolutionize trait manipulation, crop resilience, reduced environmental impact. Leveraging advancements can foster sustainable mitigate posed demands change.
Language: Английский
Citations
0
Pathogens, Journal Year: 2025, Volume and Issue: 14(4), P. 398 - 398
Published: April 21, 2025
Sclerotinia sclerotiorum is a globally widespread and vast destructive plant pathogenic fungus that causes significant yield losses in crops. Due to the lack of effective resistant germplasm resources, control diseases caused by S. largely relies on chemical fungicides. However, excessive use these chemicals not only environmental concerns but also leads increased development resistance sclerotiorum. In contrast, trans-kingdom sRNA silencing-based technologies, such as host-induced gene silencing (HIGS) spray-induced (SIGS), offer novel, effective, environmentally friendly methods for management infection. This review summarizes recent advances identification genes, target selection, categories, application RNA interference (RNAi) technologies targeting this pathogen. Although some challenges, including off-target effects efficiency external uptake, exist, findings have proposed solutions further improvement. Combined with latest developments CRISPR/Cas editing other RNAi has potential become crucial tool sclerotinia stem rot (SSR), mitigating impact crop production.
Language: Английский
Citations
0
Critical Reviews in Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 28
Published: Dec. 8, 2024
Fungal diseases threaten the forest ecosystem, impacting tree health, productivity, and biodiversity. Conventional approaches to combating diseases, such as biological control or fungicides, often reach limits regarding efficacy, resistance, non-target organisms, environmental impact, enforcing alternative approaches. From an ecological standpoint, RNA interference (RNAi) mediated double-stranded (dsRNA)-based strategy can effectively manage fungal pathogens. The RNAi approach explicitly targets suppresses gene expression through a conserved regulatory mechanism. Recently, it has evolved be effective tool in promoting sustainable management bio-fungicides provide efficient eco-friendly disease alternatives using species-specific targeting, minimizing off-target effects. With accessible data on outbreaks, genomic resources, delivery systems, RNAi-based biofungicides promising for managing pathogens forests. However, concerns fate of molecules their potential impact organisms require extensive investigation case-to-case basis. current review critically evaluates feasibility against by delving into methods, persistence, aspects, cost-effectiveness, community acceptance, plausible future protection products.
Language: Английский
Citations
2
Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2392 - 2392
Published: Nov. 22, 2024
Recent advancements in the field of plant–pathogen interactions have spotlighted role extracellular vesicles (EVs) as pivotal mediators cross-kingdom communication, offering new vistas for enhancing crop protection strategies. EVs are instrumental transport small regulatory RNAs (sRNAs) and other bioactive molecules across species boundaries, thus playing a critical molecular warfare between plants pathogens. This review elucidates sophisticated mechanisms by which utilize to dispatch sRNAs that silence pathogenic genes, fortifying defenses against microbial threats. Highlighting both eukaryotic prokaryotic systems, this delves into biogenesis, isolation, functional roles EVs, illustrating their importance not only fundamental biological processes but also potential therapeutic applications. studies illuminated significant facilitating communication pathogens, highlighting host-defense mechanisms. However, despite these advancements, challenges remain efficient isolation characterization plant-derived EVs. Overcoming is fully harnessing developing next-generation proposes innovative strategies utilizing RNA-based interventions delivered via bolster plant resilience diseases. By integrating latest scientific findings with practical applications agriculture, aims enhance connection biology development management technologies.
Language: Английский
Citations
0
Published: Dec. 10, 2024
Language: Английский
Citations
0