The Prospect of Hydrolytic Enzymes from Bacillus Species in the Biological Control of Pests and Diseases in Forest and Fruit Tree Production

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(23), P. 16889 - 16889

Published: Nov. 29, 2023

Plant diseases and insect pest damage cause tremendous losses in forestry fruit tree production. Even though chemical pesticides have been effective the control of plant pests for several decades, they are increasingly becoming undesirable due to their toxic residues that affect human life, animals, environment, as well growing challenge pesticide resistance. In this study, we review potential hydrolytic enzymes from Bacillus species such chitinases, β-1,3-glucanases, proteases, lipases, amylases, cellulases biological phytopathogens pests, which could be a more sustainable alternative pesticides. This study highlights application different sp. biocontrol alternatives against phytopathogens/insect through degradation cell wall/insect cuticles, mainly composed structural polysaccharides like chitins, β-glucans, glycoproteins, lipids. demonstrates prospects applying biopesticides forest production, mode biocidal activity dual antimicrobial/insecticidal potential, indicates great prospect simultaneous pests/diseases. Further research should focus on optimizing production enzymes, synergism facilitate

Language: Английский

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Endophytic Bacillus subtilis H17‐16 effectively inhibits Phytophthora infestans, the pathogen of potato late blight, and its potential application

Pest Management Science, Journal Year: 2023, Volume and Issue: 79(12), P. 5073 - 5086

Published: Aug. 12, 2023

As a highly prevalent epidemic disease of potato, late blight caused by Phytophthora infestans poses serious threat to potato yield and quality. At present, chemical fungicides are mainly used control blight, but long-term overuse may lead environmental pollution human health threats. Endophytes, natural resources for plant diseases control, can promote growth, enhance resistance, secrete antifungal substances. Therefore, there is an urgent need find some beneficial endophytes blight.We isolated strain Bacillus subtilis H17-16 from healthy roots. It significantly inhibit mycelial sporangia germination the pathogenicity infestans, induce resistance growth. In addition, has ability produce protease, volatile compounds (VOCs) form biofilms. After treatment, most genes involved in metabolism, virulence drug were down-regulated significantly, related ribosome biogenesis up-regulated. Moreover, field postharvest application effectively reduce occurrence combination with chitosan or had better effect than single H17-16.Our results reveal that great potential as fungicide controlling laying theoretical basis its development biological agent. © 2023 Society Chemical Industry.

Language: Английский

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Antagonistic Strain Bacillus halotolerans Jk-25 Mediates the Biocontrol of Wheat Common Root Rot Caused by Bipolaris sorokiniana

Plants, Journal Year: 2023, Volume and Issue: 12(4), P. 828 - 828

Published: Feb. 13, 2023

Common root rot caused by Bipolaris sorokiniana infestation in wheat is one of the main reasons for yield reduction crops worldwide. The bacterium strain JK-25 used current investigation was isolated from rhizosphere soil and later identified as Bacillus halotolerans based on its morphological, physiological, biochemical, molecular properties. showed significant antagonism to B. sorokiniana, Fusarium oxysporum, graminearum, Rhizoctonia zeae. Inhibition mycelial dry weight spore germination rate fermentation supernatant reached 60% 88%, respectively. crude extract found, Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), contain surfactin that exerted an inhibitory effect sorokiniana. disruption cell membranes observed under scanning confocal microscope (LSCM) after treatment mycelium with extract. antioxidant enzyme activity significantly reduced oxidation product malondialdehyde (MDA) content increased incidence pot experiments addition culture supernatant, which had a biological control 72.06%. Its ability produce siderophores may help promote growth production proteases pectinases also be part strain's role suppressing pathogens. These results demonstrate excellent antagonistic against suggest this has great potential resource strains.

Language: Английский

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Towards unlocking the biocontrol potential of Pichia kudriavzevii for plant fungal diseases: in vitro and in vivo assessments with candidate secreted protein prediction

BMC Microbiology, Journal Year: 2023, Volume and Issue: 23(1)

Published: Nov. 18, 2023

Plant fungal pathogens cause substantial economic losses through crop yield reduction and post-harvest storage losses. The utilization of biocontrol agents presents a sustainable strategy to manage plant diseases, reducing the reliance on hazardous chemical. Recently, Pichia kudriavzevii has emerged as promising agent because its capacity inhibit growth, offering potential solution for disease management.Two novel strains, Pk_EgyACGEB_O1 Pk_EgyACGEB_O2, were isolated from olive brine samples. microscopic characterization strains revealed similar structures. However, there noticeable differences in their visual morphology. Based internal transcribed spacer (ITS) DNA sequences, Pk_EgyACGEB_O2 assigned by GenBank IDs MZ507552.1 MZ507554.1 shared high sequence similarity (~ 99.8% 99.5%) with P. kudriavzevii, respectively. Both evaluated vitro against pathogenic fungi. ability consistently showing higher effectiveness. In addition, both effectively controlled grey mold caused B. cinerea golden delicious apples, suggesting eco-friendly diseases. comprehensive bioinformatics pipeline, candidate-secreted proteins responsible potent antifungal activity identified. A total 59 identified common among CBS573, SD108, SD129 strains. Approximately 23% secreted predicted secretome are hydrolases various activities, including proteases, lipases, glycosidases, phosphatases, esterases, carboxypeptidases, or peptidases. set cell-wall-related was identified, which might enhance preserving structure integrity cell wall. papain inhibitor also could potentially offer supplementary defense pathogens.Our results capabilities research focused screening growth pathogens, vivo. This study shed light how interacts pathogens. findings can help develop effective strategies managing

Language: Английский

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Isolation and Screening of Antagonistic Endophytes against Phytophthora infestans and Preliminary Exploration on Anti-oomycete Mechanism of Bacillus velezensis 6-5

Plants, Journal Year: 2023, Volume and Issue: 12(4), P. 909 - 909

Published: Feb. 17, 2023

Phytophthora infestans, the notorious pathogen of potato late blight, leads to a severe decline in yields and even harvest failure. We isolated 201 endophytic isolates from healthy root tissues potatoes, among which 41 showed strong antagonistic activity against P. infestans. Further, tolerance stress potential application blight these were tested. Most them extremely tolerant stresses such as acid-alkali, temperature, UV, salt, heavy metal stress. However, some with excellent might be pathogenic potatoes. Combining screening results, total 14 endophytes had comprehensive performance all tests. In this paper, endophyte 6-5 was selected for preliminary exploration anti-oomycete mechanism. Analysis 16S rDNA sequence revealed that high homology corresponding Bacillus velezensis (99.72%) NCBI database. Endophyte significantly inhibited mycelial growth an inhibition rate over 90% vitro assays, deformed hyphal phenotype addition, could secrete protease cellulase, produce substances thermal stability, helpful its Furthermore, it demonstrated ability improve resistance tubers blight. short, our study described process isolating infestans roots, further explored biocontrol candidate strain control.

Language: Английский

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Enhancing biological control of apple rot: Unveiling the antifungal potential and mechanism of Bacillus amyloliquefaciens HZ-12′s lipopeptide

Scientia Horticulturae, Journal Year: 2023, Volume and Issue: 325, P. 112704 - 112704

Published: Nov. 24, 2023

Language: Английский

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Machine Learning-based Host–Pathogen Protein–Protein Interaction Prediction

Current Clinical Microbiology Reports, Journal Year: 2025, Volume and Issue: 12(1)

Published: Feb. 5, 2025

Language: Английский

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Biocontrol Potential of Bacillus velezensis RS65 Against Phytophthora infestans: A Sustainable Strategy for Managing Tomato Late Blight

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 656 - 656

Published: March 14, 2025

This study aimed to investigate the biocontrol activity of rhizosphere isolates against late blight disease tomatoes caused by fungus Phytophthora infestans. A total 30 rhizospheric bacterial were evaluated for their antagonistic P. infestans in vitro and vivo. The results demonstrated that among tested, six (RS65, RP6, RS47, RS46, RP2, RS61) exhibited a highly significant inhibitory effect (p < 0.001) on mycelial growth vitro, with inhibition rate exceeding 67%. Among isolates, RS65 highest at 78.48%. For mechanisms, enzymatic activity, including proteolytic, lipolytic, chitinolytic as well production HCN, cellulase, pectinase. Isolate RS65, which showed rate, was further under greenhouse conditions. investigation revealed differences severity between control treatment. level 31.26%, whereas treatment achieved lowest 16.54%. Molecular identification indicated isolate (accession numbers PV208381) is Bacillus genus 99% proximity velezensis. finding suggests could provide effective protection infection tomato plants. These findings highlight potential agent integrated management blight.

Language: Английский

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An Eco-Friendly Bioformulation Plant Jiaosu Containing Strains with Anti-Oomycete Activity Against Phytophthora infestans

Agronomy, Journal Year: 2025, Volume and Issue: 15(4), P. 946 - 946

Published: April 13, 2025

Late blight, caused by Phytophthora infestans, is one of the most destructive diseases in potato cultivation. Chemical fungicides are currently primary means controlling it, but they pose significant issues, including environmental pollution and development resistant strains. Plant jiaosu (PJ), derived from direct fermentation various plants, plays a vital part fields such as protection, agriculture, daily chemicals, livestock. This study illustrates that PJ, fermented lettuce leaves, significantly inhibits growth P. infestans. An isolated anti-oomycete bacterium, designated X-1, has 100% homology to Bacillus subtilis NCBI database, shown through 16S rDNA sequence analysis. B. Subtilis X-1 effectively hyphal sporangia germination induces morphological changes hyphae, can promote potato. PJ exerts synergistic effect with chemical fungicide Infinito (the main active ingredients fluopicolide propamocarb hydrochloride). In summary, this provides novel approach for reutilization fruit vegetable waste offers an environmentally friendly effective alternative late blight.

Language: Английский

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Nonanol, an Induced Biomolecule Produced by Bacillus atrophaeus NMB01 During Interaction With Phytophthora infestans Can be Explored as a Novel Formulation for the Management of Late Blight of Potatoes

Journal of Basic Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

ABSTRACT Phytophthora infestans , the pathogen responsible for late blight, continues to pose a significant risk worldwide potato cultivation, including its historical impact during Irish Potato Famine. Traditional management relies heavily on synthetic fungicides, but their prolonged use has led fungicide resistance and environmental concerns. This study examines potential of bacterial endophyte Bacillus atrophaeus NMB01 as biocontrol agent against P. . Six biomolecules produced by B. were docked 15 protein targets, with 1‐nonanol, glafenine hydrochloride, mucic acid showing high binding affinity. Wet lab assays confirmed that 1‐nonanol inhibited mycelial growth 78% at 2 ppm. Molecular dynamics simulations validated stability these interactions. A tri‐trophic interaction identified additional volatile non‐volatile organic compounds (VOCs/NVOCs), minocycline doxazosin exhibiting strong across all targets. Transcriptome analysis exposed revealed differential gene expression, upregulated genes linked stress responses downregulated genes, such TAR1, cysteine synthase, glutathione transferase, presenting novel antifungal highlights promising eco‐friendly alternative conventional offering sustainable solution managing blight advancing cultivation resilience.

Language: Английский

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