The involvements of intracellular basal calcium and membrane potential in para-phenylenediamine-impaired sperm function DOI Open Access
Yannan Chen, Tingting Yang, Xiaoling Gu

et al.

Translational Andrology and Urology, Journal Year: 2024, Volume and Issue: 13(12), P. 2661 - 2671

Published: Dec. 1, 2024

Para-phenylenediamine (PPD) is a crystalline solid that belongs to the aromatic amine group, widely used in manufacturing of various dyes. PPD exhibits toxic effects on female hormone stability, ovarian function, and embryo development. Although studies have shown exposure can damage oocyte quality mice, research its male reproductive capability, particularly human sperm limited. The purpose this study was investigate effect semen explore mechanism. Computer-assisted analysis system eosin-aniline black method were conducted detect motility viability; function analyzed by tyrosine phosphorylation immunofluorescence staining, mucus penetration capacity assay, acrosome reaction incidence; reactive oxygen species (ROS) DNA specific kits; transient calcium, intracellular basal membrane potential detected multi-functional microplate reader after Fluo-4, Fura-10 AM DiSC3(5) staining. dose-dependent impact both viability sperm. Furthermore, ability capacitate, penetrate viscous substances, undergo exhibited significant impairments aspects function. comparable other bodily systems. Spermatozoon toxicity caused found be associated with increased levels ROS damage, which indicated oxidative stress plays role process. calcium response progesterone not disturbed, depolarized PPD. In summary, our findings suggest hyperpolarization damaged sperm, might underlying reasons for decline dysfunction following exposure.

Language: Английский

Human asthenozoospermia: Update on genetic causes, patient management, and clinical strategies DOI Creative Commons
Emma Cavarocchi, Maëva Drouault, João C. Ribeiro

et al.

Andrology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Abstract Background In mammals, sperm fertilization potential relies on efficient progression within the female genital tract to reach and fertilize oocyte. This fundamental property is supported by flagellum, an evolutionarily conserved organelle, which contains dynein motor proteins that provide mechanical force for propulsion motility. Primary motility of cells acquired during their transit through epididymis hyperactivated throughout journey in a process called capacitation. These activation processes rely micro‐environment tracts. particular, capacitation, panoply ion transporters located at surface mediate complex exchanges, induce increase plasma membrane fluidity, alkalinization cytoplasm protein phosphorylation cascades are compulsory hyperactivation potential. As consequence, both structural functional defects flagellum can affect motility, resulting asthenozoospermia, constitutes most predominant pathological condition associated with human male infertility. Objectives Herein, we have performed literature review comprehensive description recent advances genetics asthenozoospermia. Results Discussion We describe currently knowledge gene mutations morphology namely, asthenoteratozoospermia; also specify exclusively function activation, discuss benefit this patient couple management, terms genetic counselling diagnosis infertility as sole phenotype or association ciliary defects. Last, current strategies been initiated development therapeutical contraceptive targeting genes essential activation.

Language: Английский

Citations

0
Identification of a Novel Structural Class of HV1 Inhibitors by Structure-Based Virtual Screening DOI Creative Commons
Martina Piga, Zoltán Varga, Ádám Fehér

et al.

Journal of Chemical Information and Modeling, Journal Year: 2024, Volume and Issue: 64(12), P. 4850 - 4862

Published: June 8, 2024

The human voltage-gated proton channel, hHV1, is highly expressed in various cell types including macrophages, B lymphocytes, microglia, sperm cells and also cancer cells. Overexpression of HV1 has been shown to promote tumor formation by metastatic cells, associated with neuroinflammatory diseases, immune response disorders infertility, suggesting a potential use hHV1 inhibitors numerous therapeutic areas. To identify compounds targeting this we performed structure-based virtual screening on an open structure the channel. Twenty selected hits were tested Chinese hamster ovary (CHO) transiently expressing compound 13 showing strong block current IC50 value 8.5 μM. Biological evaluation twenty-three additional analogs led discovery six other that blocked more than 50% at 50 μM concentration. This allowed for investigation structure–activity relationships. antiproliferative activity promising was investigated lines MDA-MB-231 THP-1, where inhibited growth 9.0 8.1 μM, respectively. identification new structural class contributes our understanding requirements inhibition ion channel opens up possibility investigating role pathological conditions therapy.

Language: Английский

Citations

1
Integrated single cell transcriptome sequencing analysis reveals species‐specific genes and molecular pathways for pig spermiogenesis DOI Open Access

Y. X. Zhao,

Cai‐Xia Yang, Zhi‐Qiang Du

et al.

Reproduction in Domestic Animals, Journal Year: 2023, Volume and Issue: 58(12), P. 1745 - 1755

Published: Oct. 24, 2023

Abstract Mammalian spermatogenesis is a highly complicated and intricately organized process involving spermatogonia propagation (mitosis) meiotic differentiation into mature sperm cells (spermiogenesis). In pigs, development the role of somatic in were previously investigated detail. However, characterization key molecules fundamental to pig spermiogenesis remains less explored. Here we compared between humans focusing on spermiogenesis, by integrative testicular single‐cell RNA sequencing (scRNA‐seq) analysis. Human clustered 26 different groups, with cell‐type‐specific markers signalling pathways. For pseudo‐time analysis classified lineage routes for round, elongated spermatids spermatozoa. Moreover, molecular pathways specific each type examined respectively. Furthermore, high‐dimensional weighted gene co‐expression network (hdWGCNA) identified modules human spermatids. Hub genes (pig: SNRPD2.1 related alternative splicing; human: CATSPERZ, Ca[2+] ion channel) potentially involved also revealed. Taken together, our found that spermiogeneses involve provided resources insights further functional investigation spermatid maturation male reproductive ability.

Language: Английский

Citations

3
受精障碍导致人类不孕不育的遗传机制 DOI

Jing Dai,

YiLian HUANG,

Ge Lin

et al.

Scientia Sinica Vitae, Journal Year: 2024, Volume and Issue: 54(11), P. 2112 - 2124

Published: July 31, 2024

Citations

0
Transcriptome and proteomic analysis reveal the protective mechanism of acupuncture on reproductive function in mice with asthenospermia DOI Creative Commons

Jianheng Hao,

Jia Ren,

Boya Chang

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(17), P. e36664 - e36664

Published: Aug. 23, 2024

Acupuncture is an integral component of complementary and alternative medicine that has been reported to enhance sperm motility, improve semen quality, consequently augment male fertility. However, the precise mechanisms action underlying molecular pathways remain unclear. In present study, we aimed elucidate potential through which acupuncture improves reproductive function in a mouse model cyclophosphamide-induced asthenozoospermia. We collected from epididymis for analysis, serum determine gonadotropin oxidative stress marker levels, conducted histological examination testicular tissue using hematoxylin eosin (HE) terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, observed mitochondrial morphology transmission electron microscopy (TEM). also assessed levels total iron content validated proteomic transcriptomic analysis results real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR), protein imprinting immunohistochemistry (IHC). Our indicate enhances quality asthenozoospermic mice; increases testosterone (T), follicle-stimulating hormone (FSH), luteinizing (LH) levels; attenuates damage, accumulation, injury tissues. Through analyses, identified 21 key genes, cytochrome

Language: Английский

Citations

0
Altered Expression of Thyroid- and Calcium Ion Channels-Related Genes in Rat Testes by Short-Term Exposure to Commercial Herbicides Paraquat or 2,4-D DOI Creative Commons
Enoch Luis,

Vanessa Conde-Maldonado,

Edelmira Garcı́a-Nieto

et al.

Journal of Xenobiotics, Journal Year: 2024, Volume and Issue: 14(4), P. 1450 - 1464

Published: Oct. 9, 2024

Exposure to pesticides such as paraquat and 2,4-dichlorophenoxyacetic acid (2,4-D) has been linked harmful health effects, including alterations in male reproduction. Both herbicides are widely used developing countries have associated with reproductive alterations, disruption of spermatogenesis steroidogenesis. The thyroid axis Ca

Language: Английский

Citations

0
The involvements of intracellular basal calcium and membrane potential in para-phenylenediamine-impaired sperm function DOI Open Access
Yannan Chen, Tingting Yang, Xiaoling Gu

et al.

Translational Andrology and Urology, Journal Year: 2024, Volume and Issue: 13(12), P. 2661 - 2671

Published: Dec. 1, 2024

Para-phenylenediamine (PPD) is a crystalline solid that belongs to the aromatic amine group, widely used in manufacturing of various dyes. PPD exhibits toxic effects on female hormone stability, ovarian function, and embryo development. Although studies have shown exposure can damage oocyte quality mice, research its male reproductive capability, particularly human sperm limited. The purpose this study was investigate effect semen explore mechanism. Computer-assisted analysis system eosin-aniline black method were conducted detect motility viability; function analyzed by tyrosine phosphorylation immunofluorescence staining, mucus penetration capacity assay, acrosome reaction incidence; reactive oxygen species (ROS) DNA specific kits; transient calcium, intracellular basal membrane potential detected multi-functional microplate reader after Fluo-4, Fura-10 AM DiSC3(5) staining. dose-dependent impact both viability sperm. Furthermore, ability capacitate, penetrate viscous substances, undergo exhibited significant impairments aspects function. comparable other bodily systems. Spermatozoon toxicity caused found be associated with increased levels ROS damage, which indicated oxidative stress plays role process. calcium response progesterone not disturbed, depolarized PPD. In summary, our findings suggest hyperpolarization damaged sperm, might underlying reasons for decline dysfunction following exposure.

Language: Английский

Citations

0