Loss of LRRK2 activity induces cytoskeleton defects and oxidative stress during porcine oocyte maturation DOI Creative Commons
Yuxia Wei,

Ya-Han Wang,

Xiaoting Yu

et al.

Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 2, 2025

Leucine-rich repeat kinase 2 (LRRK2) is a ROCO family member which its mutation closely related with Parkinson's disease, and LRRK2 widely involved into the regulation of autophagy, vesicle transport neuronal proliferation. However, roles during mammalian oocyte maturation are still largely unclear. In present study, we disturbed activity showed essential in porcine oocytes. We that stably expressed maturation, loss cumulus expansion polar body extrusion, indicating involvement maturation. Further analysis indicated was cytoskeleton dynamics since inhibition caused spindle organization defect chromosome misalignment, both cytoplasmic cortex actin decreased. Moreover, co-localized mitochondria for distribution. Loss altered TMRE level, ultimately induced ROS-related oxidative stress. Taken together, our data suggested important on through effects functions.

Language: Английский

Loss of LRRK2 activity induces cytoskeleton defects and oxidative stress during porcine oocyte maturation DOI Creative Commons
Yuxia Wei,

Ya-Han Wang,

Xiaoting Yu

et al.

Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 2, 2025

Leucine-rich repeat kinase 2 (LRRK2) is a ROCO family member which its mutation closely related with Parkinson's disease, and LRRK2 widely involved into the regulation of autophagy, vesicle transport neuronal proliferation. However, roles during mammalian oocyte maturation are still largely unclear. In present study, we disturbed activity showed essential in porcine oocytes. We that stably expressed maturation, loss cumulus expansion polar body extrusion, indicating involvement maturation. Further analysis indicated was cytoskeleton dynamics since inhibition caused spindle organization defect chromosome misalignment, both cytoplasmic cortex actin decreased. Moreover, co-localized mitochondria for distribution. Loss altered TMRE level, ultimately induced ROS-related oxidative stress. Taken together, our data suggested important on through effects functions.

Language: Английский

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