To activate NAD(P)H oxidase with a brief pulse of photodynamic action DOI Creative Commons

Xiao Bing Xie,

Shu Yu,

Zong Jie Cui

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(23)

Published: Dec. 10, 2024

Reduced nicotinamide adenine dinucleotide phosphate [NAD(P)H] oxidases (NOX) are a major cellular source of reactive oxygen species, regulating vital physiological functions, whose dys-regulation leads to plethora diseases. Much effort has been made develop varied types NOX inhibitors, but biotechnologies for spatially and temporally controlled activation, however, not readily available. We previously found that ultraviolet A (UVA) irradiation activates NOX2 in rodent mast cells, elicit persistent calcium spikes. is composed multiple subunits, making studies its activation rather complicated. Here we show the single-subunit nonrodent-expressing NOX5, when expressed ectopically CHO-K1 activated by UVA (380 nm, 0.1-12 mW/cm

Language: Английский

Activation of the G protein‐coupled sulfakinin receptor inhibits blood meal intake in the mosquito Aedes aegypti DOI Creative Commons

Linlong Jiang,

Xiao Bing Xie,

Lei Zhang

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(15)

Published: Aug. 7, 2024

Abstract Little is known about the blood‐feeding physiology of arbovirus vector Aedes aegypti although this type mosquito to transmit infectious diseases dengue, Zika, yellow fever, and chikungunya. Blood feeding in female A. essential for egg maturation transmission disease agents between human subjects. Here, we identify sulfakinin receptor gene SKR from genome show that expressed at different developmental stages varied anatomical localizations adult (at three days after eclosion), with particularly high expression CNS. Knockingdown results increased blood meal intake, but microinjection thorax peptide 1 2 both inhibits dose dependently intake (and delays time course intake), which reversible antagonist. Sulfakinin ectopically mammalian cells CHO‐K1 responds stimulation persistent calcium spikes, blockable These data together suggest activation Gq protein‐coupled (i.e., calcium‐mobilizing) mosquitoes could serve as a strategic node future control reproduction/population transmission.

Language: Английский

Citations

2
To activate NAD(P)H oxidase with a brief pulse of photodynamic action DOI Creative Commons

Xiao Bing Xie,

Shu Yu,

Zong Jie Cui

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(23)

Published: Dec. 10, 2024

Reduced nicotinamide adenine dinucleotide phosphate [NAD(P)H] oxidases (NOX) are a major cellular source of reactive oxygen species, regulating vital physiological functions, whose dys-regulation leads to plethora diseases. Much effort has been made develop varied types NOX inhibitors, but biotechnologies for spatially and temporally controlled activation, however, not readily available. We previously found that ultraviolet A (UVA) irradiation activates NOX2 in rodent mast cells, elicit persistent calcium spikes. is composed multiple subunits, making studies its activation rather complicated. Here we show the single-subunit nonrodent-expressing NOX5, when expressed ectopically CHO-K1 activated by UVA (380 nm, 0.1-12 mW/cm

Language: Английский

Citations

0