Protective Effects of Recombined Mussel Adhesive Protein against AD Skin Inflammation in Mice DOI Creative Commons
Yu Wu, Feng Li,

Yan Gong

et al.

Cosmetics, Journal Year: 2024, Volume and Issue: 11(4), P. 134 - 134

Published: Aug. 9, 2024

(1) Background: Atopic dermatitis (AD) is characterized as a chronic inflammatory skin disease with significant incidence rate. The pathophysiological mechanisms underlying AD remain incompletely understood. However, extensive research demonstrates that complex interplay among genetic, immune, and environmental factors contributes to the disruption of barrier function. Inflammation identified one pathological in AD. Recombined mussel adhesive protein exhibits anti-inflammatory properties. recombinant has been used less frequently for AD, so we explored therapeutic effect potential mechanism. (2) Methods: We established mice model vivo an LPS-induced inflammation HaCaT cells vitro. Through assessment lesion scores, itch frequency, transepidermal water loss, microcirculation, HE staining, Elisa assays IL-6, IL-12, IL-13, IL-4, IL-5, IFN-γ, IgE, TNF-α, immunohistochemical staining filaggrin CK14, Masson Western blot analysis NF-κB p65, P-P65, Keap1, Nrf2, effects recombined on symptoms, pathology, inflammation, its are investigated. (3) Results: significantly improved compromised barrier, reduced scratching frequency mice, decreased lowered expression factors, thus ameliorating damage. Mechanistically, downregulated P-p65/p65 Keap1 while upregulating level Nrf2. (4) Conclusions: Overall, our results demonstrate effectiveness attenuating DNFB-induced by inhibiting activating Keap1/Nrf2 signaling pathway. Thus, promising candidate treatment

Language: Английский

Monocytes Expressing IL‐36G Play a Crucial Role in Atopic Dermatitis DOI Creative Commons

Yitao Yang,

Lei Wang,

Longmei Yu

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(7)

Published: March 30, 2025

ABSTRACT Atopic dermatitis ( ad ) is a chronic inflammatory skin disease, with recent studies indicating that immune cells, such as monocytes and cytokines, play crucial role. By retrieving datasets from public databases analysing cell infiltration in lesional using CIBERSORT, we found M2 macrophages were significantly upregulated atopic dermatitis. Differentially expressed gene (DEG) functional enrichment analysis revealed cytokine‐cytokine receptor interaction was the most enriched pathway. Further of cytokines their receptors, along correlation infiltrating identified IL36G‐expressing key target We compared cytokine‐related targets similar diseases, psoriasis urticaria, to evaluate similarities differences among these three conditions. The also highly but did not pivotal role urticaria. Finally, used molecular docking predict validate drugs targeting IL36G. Our study highlights common psoriasis, offering novel insights therapeutic strategies for related diseases.

Language: Английский

Citations

0
Screening for Systemic Diseases Associated with Dental Self-Care in Japanese Adolescents DOI Open Access
Masanobu Abe, Akihisa Mitani, Kazuto Hoshi

et al.

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(20), P. 6087 - 6087

Published: Oct. 12, 2024

Toothbrushing is important for maintaining oral health and preventing periodontal disease. However, the association between toothbrushing systemic diseases remains unclear in adolescence. In this study, dental self-care (frequency duration of toothbrushing) diseases/disorders adolescents was examined.

Language: Английский

Citations

1
Protective Effects of Recombined Mussel Adhesive Protein against AD Skin Inflammation in Mice DOI Creative Commons
Yu Wu, Feng Li,

Yan Gong

et al.

Cosmetics, Journal Year: 2024, Volume and Issue: 11(4), P. 134 - 134

Published: Aug. 9, 2024

(1) Background: Atopic dermatitis (AD) is characterized as a chronic inflammatory skin disease with significant incidence rate. The pathophysiological mechanisms underlying AD remain incompletely understood. However, extensive research demonstrates that complex interplay among genetic, immune, and environmental factors contributes to the disruption of barrier function. Inflammation identified one pathological in AD. Recombined mussel adhesive protein exhibits anti-inflammatory properties. recombinant has been used less frequently for AD, so we explored therapeutic effect potential mechanism. (2) Methods: We established mice model vivo an LPS-induced inflammation HaCaT cells vitro. Through assessment lesion scores, itch frequency, transepidermal water loss, microcirculation, HE staining, Elisa assays IL-6, IL-12, IL-13, IL-4, IL-5, IFN-γ, IgE, TNF-α, immunohistochemical staining filaggrin CK14, Masson Western blot analysis NF-κB p65, P-P65, Keap1, Nrf2, effects recombined on symptoms, pathology, inflammation, its are investigated. (3) Results: significantly improved compromised barrier, reduced scratching frequency mice, decreased lowered expression factors, thus ameliorating damage. Mechanistically, downregulated P-p65/p65 Keap1 while upregulating level Nrf2. (4) Conclusions: Overall, our results demonstrate effectiveness attenuating DNFB-induced by inhibiting activating Keap1/Nrf2 signaling pathway. Thus, promising candidate treatment

Language: Английский

Citations

0