Association between aging-dependent gut microbiome dysbiosis and dry eye severity in C57BL/6 male mouse model: a pilot study DOI Creative Commons
Chang Ho Yoon,

Jin Suk Ryu,

Jayoon Moon

et al.

BMC Microbiology, Journal Year: 2021, Volume and Issue: 21(1)

Published: April 9, 2021

Abstract Background While aging is a potent risk factor of dry eye disease, age-related gut dysbiosis associated with inflammation and chronic geriatric diseases. Emerging evidence have demonstrated that contributes to the pathophysiology or exacerbation ocular diseases including disease. However, relationship between aging-related changes in microbiota disease has not been elucidated. In this pilot study, we investigated association aging-dependent microbiome severity C57BL/6 male mice. Results Eight-week-old (8 W, n = 15), one-year-old (1Y, 10), two-year-old (2Y, 8) mice were used. Dry was assessed by corneal staining scores tear secretion. Bacterial genomic 16 s rRNA from feces analyzed. Main outcomes compositional differences among groups their correlation severity. aged (1Y 2Y), increased secretion decreased statistical significance. Gut α-diversity different groups. β-diversity significantly univariate analysis, phylum Firmicutes , Proteobacteria, Cyanobacteria / Bacteroidetes ratio, genus Alistipes Bacteroides Prevotella Paraprevotella Helicobacter related After adjustment age, multivariate analysis revealed Proteobacteria Lactobacillus be Conclusions Our study suggests composition are signs

Language: Английский

Pain, Dryness, and Itch Sensations in Eye Surface Disorders Are Defined By a Balance Between Inflammation and Sensory Nerve Injury DOI
Carlos Belmonte

Cornea, Journal Year: 2019, Volume and Issue: 38(1), P. S11 - S24

Published: Sept. 4, 2019

Abstract: Patients with corneal and conjunctival disorders report an array of ocular surface symptoms including stinging, foreign body sensation, itching. The intensity perceptual quality these sensations their duration, from brief intervals to long-term symptoms, also vary. We hypothesize that symptomatic differences across reflect in the balance between inflammation nerve injury, different conditions resulting predominant effects one these, or a combined effect. This article provides overview cells, such as nociceptors thermoreceptors, descriptions morphological molecular characteristics nerve-firing patterns evoked sensations, determined by earlier studies animals humans. Detailed changes neuronal responses (such abnormal responsiveness spontaneous firing) due local injury are provided, assorted discussed. Eye which is include allergic conjunctivitis photokeratitis, whereas primary factor underlying complaints dry eye after photorefractive keratectomy elderly patients. Both factors contribute substantially disease varicella-zoster infections. model serves explain reported various disorders, short-term versus chronic pain dysesthesias, may help improve diagnoses treatment methods.

Language: Английский

Citations

47
Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse Model DOI Open Access
Chang Ho Yoon,

Jin Suk Ryu,

Ho Sik Hwang

et al.

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(11), P. 4169 - 4169

Published: June 11, 2020

It is not known how biological changes in the lacrimal (LGs) and meibomian (MGs) glands contribute to dry eye disease (DED) a time-dependent manner. In this study, we investigated time-sequenced inflammation, oxidative stress, senescence of stem cells both an aging-related DED mouse model. Eight-week (8W)-, one-year (1Y)-, two-year (2Y)-old C57BL/6 male mice were used. MG areas upper lower eyelids analyzed by transillumination meibography imaging. The number CD45+, 8-OHdG+, Ki-67+, BrdU+ was compared glands. Increased corneal staining decreased tear secretion observed aged mice. dropout area increased with aging, age-adjusted lids negatively correlated National Eye Institute (NEI) score. CD4+ interferon (IFN)-γ+ LGs found An increase 8-OHdG+ evident 2Y-old Reduced Ki-67+ cells, but no change CD45+ MGs 1Y-old This suggests that age-dependent related inflammation LGs, development atrophy, stress

Language: Английский

Citations

41
Is dry eye disease the same in young and old patients? A narrative review of the literature DOI Creative Commons
Stefano Barabino

BMC Ophthalmology, Journal Year: 2022, Volume and Issue: 22(1)

Published: Feb. 22, 2022

Abstract Advanced age is one of the most evident risk factors for dry eye disease (DED), with male/female sex, chronic drug consumption, and prolonged device use. This article aims to review literature about changes ocular surface associated DED in elderly patients < 40 years. The pathophysiologic responsible dryness are linked inflammation neurosensory abnormalities may occur a different feature young compared elders. Peculiar treatment strategies be needed older subjects DED.

Language: Английский

Citations

26
Novel nanostructured lipid carriers loading Apigenin for anterior segment ocular pathologies DOI Creative Commons

Lorena Bonilla-Vidal,

Marta Espina, María L. García

et al.

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: 658, P. 124222 - 124222

Published: May 11, 2024

Dry eye disease (DED) is a chronic multifactorial disorder of the ocular surface caused by tear film dysfunction and constitutes one most common conditions worldwide. However, its treatment remains unsatisfactory. While artificial tears are commonly used to moisturize surface, they do not address underlying causes DED. Apigenin (APG) natural product with anti-inflammatory properties, but low solubility bioavailability limit efficacy. Therefore, novel formulation APG loaded into biodegradable biocompatible nanoparticles (APG-NLC) was developed overcome restricted stability, improve therapeutic efficacy, prolong retention time on extending release. APG-NLC optimization, characterization, biopharmaceutical properties efficacy were evaluated. The optimized exhibited an average particle size below 200 nm, positive charge, encapsulation efficiency over 99 %. sustained release APG, stability studies demonstrated that retained integrity for 25 months. In vitro in vivo tolerance indicated did cause any irritation, rendering them suitable topical administration. Furthermore, showed non-toxicity epithelial corneal cell line fast internalization. Therapeutic benefits using model DED, where effectively reversed DED reducing cellular damage increasing volume. Anti-inflammatory assays also showcased potential treat prevent inflammation, particularly relevant patients. Hence, represent promising system prevention associated inflammation.

Language: Английский

Citations

5
Lights and Shadows of Cytokines in Age‐Related Eye Diseases: A Narrative Literature Review DOI Open Access

Yuyu Xue,

Lu Ye,

Chan Huang

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(1)

Published: Jan. 1, 2025

ABSTRACT The eye is considered to be an immune‐privileged region. However, several parts of the have distinct mechanisms for delivering immune cells injury sites or even in response aging. Although these responses are intended protective, visual acuity can compromised by release pro‐inflammatory cytokines cells, which induce chronic inflammation and fibrosis. Age‐related diseases (AREDs) primary cause vision impairment (VI) elderly, with a poor comprehension their pathophysiology. affect both anterior posterior segments, resulting diminished quality life risk irreversible blindness. Immune system dysregulation upregulation been linked AREDs, underscoring need comprehend inflammation's impact on ocular disorders enhance patient symptom management. In this framework, PubMed database was searched using medical subject headings (MeSH) terms “Age‐related diseases,” “dry syndrome,” “glaucoma,” “cataract,” “diabetic retinopathy,” “inflammation,” “interleukin,” “cytokine” aim overview role AREDs discuss potential therapeutic approaches.

Language: Английский

Citations

0
Association of ocular adverse events with varenicline solution use: a population-based study DOI Creative Commons
Moiz Lakhani, Angela T.H. Kwan, Anne Xuan-Lan Nguyen

et al.

Expert Opinion on Drug Safety, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 9

Published: Feb. 8, 2025

Background Approved by the FDA in 2021, varenicline solution is first nasal spray specifically designed to enhance basal tear film production for treating dry eye disease (DED). However, there a lack of data comprehensively comparing its safety profile conventional DED therapies. Herein, we assess whether ocular adverse events (AEs) are disproportionately reported with real-world use solution.

Language: Английский

Citations

0
Comparison of keratometry assessment between IOLMaster and Pentacam in dry eyes DOI Creative Commons
Fei Yang, Jiayin Qin, Xijuan Wang

et al.

Photodiagnosis and Photodynamic Therapy, Journal Year: 2025, Volume and Issue: unknown, P. 104527 - 104527

Published: Feb. 1, 2025

To compare the keratometry measurements between IOLMaster and Pentacam in dry eyes. A total of 122 eyes 95 age-related cataract patients with eye disease were enrolled this study. Keratometry including flat steep (K), mean keratometry, axis K, astigmatism anterior corneal surface (A-ACS) obtained using Pentacam. second series examinations performed 1 minute after instillation artificial tears. Values measured by two devices compared both before Changes above parameters each device also analyzed. The K was significantly lower than A-ACS larger Pentacam, a discrepancy 0.46 D an upper limit 95% LoA 1.48 D. for exceeded clinically relevant margin. modified None showed significant difference except A-ACS, decreased to 0.17 -0.20 0.54 Intra comparison that changes greater those Dry has impact on For cases over 0.5 D, tears are recommended performing biometry.

Language: Английский

Citations

0
Cytokines in age-related eye diseases: pathogenesis and potential targets for innovative therapies DOI
Tingting Zhang,

Ming Liu

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Lymphocyte Subpopulations in the Healthy Human Lacrimal Gland and Their Variations With Age and Sex, Systematic Review 1960–2023 DOI Creative Commons
Claudia M. Trujillo‐Vargas,

Luisa María Rendón‐Macías,

Roberto Peña Guerrero

et al.

Immunity Inflammation and Disease, Journal Year: 2025, Volume and Issue: 13(3)

Published: March 1, 2025

ABSTRACT Background Immunosenescence has been associated with an imbalance in the lacrimal functional unit and histopathological changes exocrine glands, especially women. Objective To define main lymphocyte subpopulations human gland their variations age sex, according to scientific articles published between 1960 2023. Methods A systematic review was performed on PubMed, ScienceDirect, Google Scholar. The PRISMA 2020 guidelines were applied for search selection of studies. methodological quality evaluated STROBE guidelines. meta‐analysis three selected dichotomizing lymphocytic infiltrates group also performed. Results We 20 observational studies, including 774 healthy individuals (722 cadavers). infiltration hematoxylin eosin staining, immunohistochemistry immunofluorescence. There high variability criteria apparently quantify infiltration. underrepresentation younger than 40 years (12.6%), female sex (38.9%). Three reported association gland, while two did not. Plasma cells most abundant subpopulation IgA‐containing plasma cells. B be very scarce LG articles. In articles, no statistical difference found older 60. Conclusion is need further better defining study design, similar representation across ages ascertain what are composition related sex. Further studies needed assess dynamics populations a more detailed manner using cutting‐edge methodologies such as single‐cell sequencing or transcriptomics. Trial Registration PROSPERO 2023 CRD42023435653 Available from: https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023435653 .

Language: Английский

Citations

0
Patients’ knowledge, attitudes, and practices regarding lifestyle related dry eye DOI Creative Commons
Ke Song,

Shanshan Ye,

Shujiao Li

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 8, 2025

Rising incidences of dry eye, often attributed to modern lifestyle and environmental factors, highlight the need for knowledge, attitudes, practices (KAP) studies inform tailored interventions. This study evaluated patients' KAP concerning lifestyle-related eye at Ophthalmology Hospital China Academy Chinese Medical Sciences from July 1 26, 2024. A self-designed questionnaire was used gather demographic data assess scores, yielding 556 valid responses (98.93%). Among participants, 342 (61.51%) were female, with a mean age 39.26 ± 13.56 years. Mean scores 7.44 4.65 (knowledge), 35.20 4.10 (attitude), 35.77 6.15 (practice). Mediation analysis indicated direct influences familial history on knowledge impacting attitude, various factors influencing practice. While patients exhibited limited they generally held positive attitudes engaged in proactive practices. Enhancing patient education related is essential boost foster effective prevention management strategies.

Language: Английский

Citations

0