Protective Effects of Schizochytrium Microalgal Fatty Acids on Alcoholic Liver Disease: A Network Pharmacology and In Vivo Study DOI

Cailin Luo,

Li Tian,

Yangmin Wen

et al.

Assay and Drug Development Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

This study aimed to elucidate the hepatoprotective mechanisms of microalgal fatty acids (MFA) from Schizochytrium against alcoholic liver disease (ALD) through network pharmacology and in vivo analysis. Network molecular docking methodologies were employed predict potential MFA ALD. To substantiate these predictions, an acute injury mouse model was utilized assess impact on serum levels alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate (AST), total protein (TP), albumin (ALB). Additionally, histopathology expression phosphatidylinositol 3 kinase (PI3K) B (AKT) evaluated. Seven active ingredients 53 targets (including 7 core targets) for ALD treatment identified MFA. Kyoto Encyclopedia Genes Genomes pathway analyses indicated that seven are implicated various biological pathways, notably those associated with cancer, viral infections, PI3K/AKT signaling pathway. Furthermore, studies demonstrated docosahexaenoic acid docosapentaenoic exhibited strong binding affinity crucial targets. Animal experiments administration significantly decreased AST, ALT, ALP, while increasing ALB TP mice injury. Moreover, ameliorated tissue pathology markedly down-regulated PI3K AKT proteins liver. These results suggest may possess therapeutic by targeting multiple its likely involving inhibition

Language: Английский

Protective Effects of Schizochytrium Microalgal Fatty Acids on Alcoholic Liver Disease: A Network Pharmacology and In Vivo Study DOI

Cailin Luo,

Li Tian,

Yangmin Wen

et al.

Assay and Drug Development Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

This study aimed to elucidate the hepatoprotective mechanisms of microalgal fatty acids (MFA) from Schizochytrium against alcoholic liver disease (ALD) through network pharmacology and in vivo analysis. Network molecular docking methodologies were employed predict potential MFA ALD. To substantiate these predictions, an acute injury mouse model was utilized assess impact on serum levels alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate (AST), total protein (TP), albumin (ALB). Additionally, histopathology expression phosphatidylinositol 3 kinase (PI3K) B (AKT) evaluated. Seven active ingredients 53 targets (including 7 core targets) for ALD treatment identified MFA. Kyoto Encyclopedia Genes Genomes pathway analyses indicated that seven are implicated various biological pathways, notably those associated with cancer, viral infections, PI3K/AKT signaling pathway. Furthermore, studies demonstrated docosahexaenoic acid docosapentaenoic exhibited strong binding affinity crucial targets. Animal experiments administration significantly decreased AST, ALT, ALP, while increasing ALB TP mice injury. Moreover, ameliorated tissue pathology markedly down-regulated PI3K AKT proteins liver. These results suggest may possess therapeutic by targeting multiple its likely involving inhibition

Language: Английский

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