A Brief History of Cell Culture: From Harrison to Organs-on-a-Chip DOI Creative Commons
Lincoln Gozzi Moro, Lucas Pires Guarnier, Maurício Fogaça Azevedo

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2068 - 2068

Published: Dec. 15, 2024

This comprehensive overview of the historical milestones in cell culture underscores key breakthroughs that have shaped field over time. It begins with Wilhelm Roux’s seminal experiments 1880s, followed by pioneering efforts Ross Granville Harrison, who initiated groundbreaking fundamentally landscape early 20th century. Carrel’s influential contributions, notably immortalization chicken heart cells, marked a significant advancement techniques. Subsequently, Johannes Holtfreter, Aron Moscona, and Joseph Leighton introduced methodological innovations three-dimensional (3D) culture, Alexis Carrel, laying groundwork for future consolidation expansion use 3D different areas biomedical sciences. The advent induced pluripotent stem cells Takahashi Yamanaka 2006 was revolutionary, enabling reprogramming differentiated into state. Since then, recent included spheroids, organoids, organ-on-a-chip technologies, aiming to mimic structure function tissues organs vitro, pushing boundaries biological modeling disease understanding. In this review, we history shedding light on main discoveries, pitfalls hurdles were overcome during transition from 2D Finally, discussed directions research may accelerate development more effective personalized treatments.

Language: Английский

Stable, Conductive, Adhesive Polymer Patterning Inside a Microfluidic Chamber for Endothelial Cell Alignment DOI Creative Commons
Elena Mancinelli, Silvia Taccola, Ellen Slay

et al.

Advanced Materials Technologies, Journal Year: 2024, Volume and Issue: unknown

Published: June 11, 2024

Abstract Endothelial cells (ECs) line the inner walls of blood vessels, respond to shear stress by elongating in direction flow. Engineering aligned ECs vitro is essential for modeling human vascular diseases and drug testing. Current microfluidic approaches mainly rely on unidirectional laminar flow, uniform coating surfaces improve cellular adhesion or alteration surface topography. Challenges persist due stress‐induced changes behavior, especially complex multicellular environments time needed align polarize inside conduits. Generally, protein processes physical treatments are also not compatible with steps required assembly devices. This approach employs aerosol jet printing (AJP) precisely pattern poly(3,4‐ethylenedioxythiophene) polystyrene sulphonate (PEDOT:PSS) within chambers a single step. It shown that PEDOT:PSS biocompatible facilitates EC adhesion, patterning, elongation, alignment. Under capillary retain their pattern‐induced morphology over 7 d, confirming efficacy promoting organization, eliminating need external pumps. Furthermore, it demonstrated retains structural integrity electrical stability following oxygen plasma treatment, assembling fully enclosed

Language: Английский

Citations

1
A Brief History of Cell Culture: From Harrison to Organs-on-a-Chip DOI Creative Commons
Lincoln Gozzi Moro, Lucas Pires Guarnier, Maurício Fogaça Azevedo

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2068 - 2068

Published: Dec. 15, 2024

This comprehensive overview of the historical milestones in cell culture underscores key breakthroughs that have shaped field over time. It begins with Wilhelm Roux’s seminal experiments 1880s, followed by pioneering efforts Ross Granville Harrison, who initiated groundbreaking fundamentally landscape early 20th century. Carrel’s influential contributions, notably immortalization chicken heart cells, marked a significant advancement techniques. Subsequently, Johannes Holtfreter, Aron Moscona, and Joseph Leighton introduced methodological innovations three-dimensional (3D) culture, Alexis Carrel, laying groundwork for future consolidation expansion use 3D different areas biomedical sciences. The advent induced pluripotent stem cells Takahashi Yamanaka 2006 was revolutionary, enabling reprogramming differentiated into state. Since then, recent included spheroids, organoids, organ-on-a-chip technologies, aiming to mimic structure function tissues organs vitro, pushing boundaries biological modeling disease understanding. In this review, we history shedding light on main discoveries, pitfalls hurdles were overcome during transition from 2D Finally, discussed directions research may accelerate development more effective personalized treatments.

Language: Английский

Citations

0