Masterclass in neuroendocrinology, Journal Year: 2025, Volume and Issue: unknown, P. 45 - 70
Published: Jan. 1, 2025
Language: Английский
Frontiers in Endocrinology, Journal Year: 2018, Volume and Issue: 9
Published: Oct. 25, 2018
The nervous system of the free-living planktonic larvae sea urchins is relatively "simple", but sufficiently complex to enable sensing environment and control swimming feeding behaviours. At pluteus stage development, comprises a central ganglion serotonergic neurons located in apical organ sensory motor associated with ciliary band gut. Neuropeptides are key mediators neuronal signaling systems currently little known about neuropeptidergic urchin larvae. Analysis genome sequence Strongylocentrotus purpuratus has enabled identification thirty-eight genes encoding neuropeptide precursors (NP) this species. Here we characterise for first time expression nine these NP S. larvae, providing basis functional understanding neurochemical organization larval system. In order accomplish used single double situ hybridization, coupled immunohistochemistry, investigate gene comparison markers (e.g. neurotransmitter serotonin). Several sub-populations cells that express one or more were identified, which plate, at base arms, around mouth, mid- fore-gut. Furthermore, high levels cell proliferation observed neurogenic territories, consistent an increase number late stages. This study revealed far level than was previously known. Our map provides future work, aimed role diverse neuropeptides various aspects embryonic behaviour.
Language: Английский
Citations
35
Open Biology, Journal Year: 2020, Volume and Issue: 10(9)
Published: Sept. 1, 2020
Somatostatin (SS) and allatostatin-C (ASTC) are structurally evolutionarily related neuropeptides that act as inhibitory regulators of physiological processes in mammals insects, respectively. Here, we report the first molecular functional characterization SS/ASTC-type signalling a deuterostome invertebrate—the starfish Asterias rubens (phylum Echinodermata). Two precursors were identified A. (ArSSP1 ArSSP2) structures derived from these proteins (ArSS1 ArSS2) analysed using mass spectrometry. Pharmacological three cloned receptors (ArSSR1–3) revealed ArSS2, but not ArSS1, acts ligand for all receptors. Analysis ArSS2 expression mRNA situ hybridization immunohistochemistry stained cells/fibres central nervous system, digestive system (e.g. cardiac stomach) body wall its appendages tube feet). Furthermore, vitro pharmacological tests causes dose-dependent relaxation foot stomach preparations, while injection vivo partial eversion stomach. Our findings provide new insights into evolution animal kingdom reveal an ancient role SS-type muscle contractility.
Language: Английский
Citations
32
FEBS Journal, Journal Year: 2021, Volume and Issue: 288(8), P. 2727 - 2745
Published: Jan. 13, 2021
The increasing human population, combined with low inefficiency and adverse effects of available pesticides, has magnified the urgent need developing next‐generation pesticides. Among approaches, strategies targeting invertebrate G protein‐coupled receptors (GPCRs) are very promising as these targets endogenous neuropeptides/neuromodulators that upon binding to their induce profound changes in insect physiology. Therefore, exploring GPCR regulators great potential development targeted Despite such alternative so far there been only one approved compound, Amitraz, which conveys its anti‐pest activity via Octopamine receptor. Here, we review current status pesticide development, hazards associated conventional compounds, involve structural features GPCRs, opportunities challenges members this superfamily invertebrates develop agents. In conclusion, emphasize GPCRs cannot be utilized full without more genomic transcriptomic data improve our understanding complex network peptidergic signaling pathways. We argue how vital it is obtain three‐dimensional (3D) on physiologically important target encourage readers use state art silico methods virtual screening for discovery new compounds.
Language: Английский
Citations
24
General and Comparative Endocrinology, Journal Year: 2025, Volume and Issue: unknown, P. 114716 - 114716
Published: March 1, 2025
Language: Английский
Citations
0
Masterclass in neuroendocrinology, Journal Year: 2025, Volume and Issue: unknown, P. 45 - 70
Published: Jan. 1, 2025
Language: Английский
Citations
0