Planar cell polarity: intracellular asymmetry and supracellular gradients of Frizzled DOI Creative Commons
José Casal,

Freya Storer,

Peter A. Lawrence

et al.

Open Biology, Journal Year: 2023, Volume and Issue: 13(6)

Published: June 1, 2023

Planar cell polarity (PCP), the coordinated orientation of structures such as cilia, mammalian hairs or insect bristles, depends on at least two molecular systems. We have argued that these systems use similar mechanisms; each depending a supracellular gradient concentration spans field cells. In linked paper, we studied Dachsous/Fat system. found graded distribution Dachsous in vivo segment pupal epidermis abdomen Drosophila. Here report study key molecule for Starry Night/Frizzled 'core' measure receptor Frizzled membranes all cells one living find falls about 17% from front to rear segment. present some evidence then resets most anterior next back. an intracellular asymmetry cells, posterior membrane carrying 22% more than membrane. These direct measurements add earlier PCP act independently.

Language: Английский

The wing imaginal disc DOI Creative Commons
Bipin Kumar Tripathi, Kenneth D. Irvine

Genetics, Journal Year: 2022, Volume and Issue: 220(4)

Published: March 4, 2022

Abstract The Drosophila wing imaginal disc is a tissue of undifferentiated cells that are precursors the and most notum adult fly. first forms during embryogenesis from cluster ∼30 located in second thoracic segment, which invaginate to form sac-like structure. They undergo extensive proliferation larval stages mature ∼35,000 cells. During this time, distinct cell fates assigned different regions, develops complex morphology. Finally, pupal undergoes morphogenetic processes then differentiates notum. While bulk comprises epithelial cells, it also includes neurons glia, associated with tracheal muscle precursor relative simplicity accessibility disc, combined wealth genetic tools available Drosophila, have make premier system for identifying genes deciphering systems play crucial roles animal development. Studies discs made key contributions many areas biology, including patterning, signal transduction, growth control, regeneration, planar polarity, morphogenesis, mechanics.

Language: Английский

Citations

77
Planar cell polarity coordination in a cnidarian embryo provides clues to animal body axis evolution DOI Open Access

Julie Uveira,

Antoine Donati,

Marvin Léria

et al.

Published: Jan. 28, 2025

Body axis specification is a crucial event in animal embryogenesis and was an essential evolutionary innovation for founding the kingdom. It involves two distinct components that coordinate to establish spatial organisation of embryo: initiation cascades regionalised gene expression orientation morphogenetic processes such as body elongation. Intense interest first component has revealed Wnt/β-catenin signalling ancestrally responsible initiating regional expression, but origin oriented morphogenesis received little attention. Here, by addressing cell morphological basis development embryos cnidarian Clytia hemisphaerica, we have uncovered simple likely ancestral coordination mechanism between directed morphogenesis. We show ligand Wnt3, known initiate oral via localised pathway activation, also key β-catenin-independent role globally orienting planar polarity (PCP) direct along oral-aboral axis. This PCP occurs steps: local Wnt3 global propagation conserved core protein interactions From these findings propose novel scenarios PCP-driven symmetry-breaking underlying emergence plan.

Language: Английский

Citations

1
Wnt-Frizzled planar cell polarity signaling in the regulation of cell motility DOI
Yildiz Koca, Giovanna M. Collu, Marek Mlodzik

et al.

Current topics in developmental biology/Current Topics in Developmental Biology, Journal Year: 2022, Volume and Issue: unknown, P. 255 - 297

Published: Jan. 1, 2022

Language: Английский

Citations

33
Planar cell polarity regulators in asymmetric organogenesis during development and disease DOI Creative Commons

De‐Li Shi

Journal of genetics and genomics/Journal of Genetics and Genomics, Journal Year: 2022, Volume and Issue: 50(2), P. 63 - 76

Published: July 6, 2022

Language: Английский

Citations

19
Crustacean leg regeneration restores complex microanatomy and cell diversity DOI Creative Commons
Alba Almazán, Çağrı Çevrim, Jacob M. Musser

et al.

Science Advances, Journal Year: 2022, Volume and Issue: 8(34)

Published: Aug. 24, 2022

Animals can regenerate complex organs, yet this process frequently results in imprecise replicas of the original structure. In crustacean Parhyale , embryonic and regenerating legs differ gene expression dynamics but produce apparently similar mature structures. We examine fidelity leg regeneration using complementary approaches to investigate microanatomy, sensory function, cellular composition, cell molecular profiles. find that precisely replicates microanatomy spatial distribution external organs restores their function. Single-nuclei sequencing shows regenerated uninjured are indistinguishable terms cell-type composition transcriptional This remarkable highlights ability organisms achieve identical outcomes via distinct processes.

Language: Английский

Citations

17
Molecular symmetry breaking in the Frizzled-dependent planar polarity pathway DOI Creative Commons
Helen Strutt, Samantha J. Warrington,

Amritha Chemmenchery Kokkam Madathil

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(24), P. 5340 - 5354.e6

Published: Nov. 22, 2023

The core planar polarity pathway consists of six proteins that form asymmetric intercellular complexes segregate to opposite cell ends in developing tissues and specify polarized structures or behaviors. Within these complexes, the atypical cadherin Flamingo localizes on both sides junctions, where it interacts homophilically trans via its repeats, whereas transmembrane Frizzled Strabismus localize apposing junctions. However, molecular mechanisms underlying formation such are poorly understood. Using a novel tissue culture system, we determine minimum requirements for complex assembly absence confounding feedback mechanisms. We show intrinsically an interaction one with neighboring is key symmetry-breaking step. In contrast, unable promote homophilic binding only recruited into once has entered side. This requires intact intracellular loops seven-pass domain Flamingo. Once recruited, stabilizes together three cytoplasmic proteins. propose model whereby exists closed conformation results conformational change allows repeats interact molecule cell. adjacent then undergoes further region promotes recruitment Strabismus.

Language: Английский

Citations

10
Planar cell polarity coordination in a cnidarian embryo provides clues to animal body axis evolution DOI Open Access

Julie Uveira,

Antoine Donati,

Marvin Léria

et al.

Published: Jan. 28, 2025

Body axis specification is a crucial event in animal embryogenesis and was an essential evolutionary innovation for founding the kingdom. It involves two distinct components that coordinate to establish spatial organisation of embryo: initiation cascades regionalised gene expression orientation morphogenetic processes such as body elongation. Intense interest first component has revealed Wnt/β-catenin signalling ancestrally responsible initiating regional expression, but origin oriented morphogenesis received little attention. Here, by addressing cell morphological basis development embryos cnidarian Clytia hemisphaerica, we have uncovered simple likely ancestral coordination mechanism between directed morphogenesis. We show ligand Wnt3, known initiate oral via localised pathway activation, also key β-catenin-independent role globally orienting planar polarity (PCP) direct along oral-aboral axis. This PCP occurs steps: local Wnt3 global propagation conserved core protein interactions From these findings propose novel scenarios PCP-driven symmetry-breaking underlying emergence plan.

Language: Английский

Citations

0
Interacting Fat1 and Dchs Planar Cell Polarity Proteins Supported by Fjx1 Serve as Heterodimeric Intercellular Bridges Crucial to Support Spermatogenesis DOI

Tiao Bu,

Lingling Wang, Xiaolong Wu

et al.

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown, P. 355 - 374

Published: Jan. 1, 2025

Language: Английский

Citations

0
Fat body-derived cytokine Upd2 controls disciplined migration of tracheal stem cells in Drosophila DOI Open Access

Pengzhen Dong,

Yue Li, Yuying Wang

et al.

Published: April 30, 2025

Coordinated activation and directional migration of adult stem cells are essential for maintaining tissue homeostasis. Drosophila tracheal progenitors that migrate posteriorly along the dorsal trunk to replenish degenerating branches disperse FGF mitogen. However, it is currently unknown how overall anterior-to-posterior directionality such controlled. Here we show individual progenitor together in a concerted, disciplined manner, behavior dependent on neighboring fat body. We identify body-derived cytokine, Upd2, targeting inducing JAK/STAT signaling maintain their migration. Perturbation either Upd2 production body or trachea causes aberrant bidirectional progenitors. promotes expression genes involved planar cell polarity leading asymmetric localization Fat cells. provide evidence transport requires Rab5- Rab7-mediated endocytic sorting Lbm-dependent vesicle trafficking. Our study thus uncovers an inter-organ communication control cells, process vesicular trafficking cytokine signaling-mediated PCP genes.

Language: Английский

Citations

0
Emerging Mechanisms of Growth and Patterning Regulation by Dachsous and Fat Protocadherins DOI Creative Commons

Artem Gridnev,

Jyoti R. Misra

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: March 16, 2022

Dachsous (Ds) and Fat are evolutionarily conserved cell adhesion molecules that play a critical role in development of multiple organ systems, where they coordinate tissue growth morphogenesis. Much our understanding Ds-Fat signaling pathway comes from studies Drosophila , initiate regulate by influencing Hippo morphogenesis regulating Planar Cell Polarity (PCP). In this review, we discuss recent advances the mechanisms which regulates these developmental processes. Further, progress about how function mammals.

Language: Английский

Citations

15