A feather star is born: embryonic development and nervous system organization in the crinoid Antedon mediterranea DOI Creative Commons
Silvia Mercurio, Giacomo Gattoni, Giorgio Scarı̀

et al.

Open Biology, Journal Year: 2024, Volume and Issue: 14(8)

Published: Aug. 1, 2024

Crinoids belong to the Echinodermata, marine invertebrates with a highly derived adult pentaradial body plan. As sister group all other extant echinoderms, crinoids occupy key phylogenetic position explore evolutionary history of whole phylum. However, their development remains understudied compared that echinoderms. Therefore, aim here was establish Mediterranean feather star ( Antedon mediterranea ) as an experimental system for developmental biology. We first set up method culturing embryos in vitro and defined standardized staging this species. then optimized protocols characterize morphological molecular main structures Focusing on nervous system, we showed larval apical organ includes serotonergic, GABAergic glutamatergic neurons, which develop within conserved anterior signature. described composition early post-metamorphic revealed it has These results further our knowledge crinoid provide new techniques investigate embryogenesis. This will pave way inclusion comparative studies addressing origin echinoderm plan diversification deuterostomes.

Language: Английский

An ancient apical patterning system sets the position of the forebrain in chordates DOI Creative Commons
Giacomo Gattoni, Daniel Keitley, Ashley Sawle

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(4)

Published: Jan. 24, 2025

The evolutionary origin of the vertebrate brain remains a major subject debate, as its development from dorsal tubular neuroepithelium is unique to chordates. To shed light on emergence brain, we compared anterior neuroectoderm across deuterostome species, using available single-cell datasets sea urchin, amphioxus, and zebrafish embryos. We identified conserved gene co-expression module, comparable regulatory network (aGRN) controlling apical organ in ambulacrarians, spatially mapped it by multiplexed situ hybridization developing retina hypothalamus Using functional approaches, show Wnt signaling regulating this module like aGRN echinoderms, that overactivation suppresses forebrain identity. This suggests previously undescribed role for amphioxus determining position forebrain. propose Wnt-regulated possible mechanism which set antero-dorsally early chordate evolution.

Language: Английский

Citations

0
A feather star is born: embryonic development and nervous system organization in the crinoid Antedon mediterranea DOI Creative Commons
Silvia Mercurio, Giacomo Gattoni, Giorgio Scarı̀

et al.

Open Biology, Journal Year: 2024, Volume and Issue: 14(8)

Published: Aug. 1, 2024

Crinoids belong to the Echinodermata, marine invertebrates with a highly derived adult pentaradial body plan. As sister group all other extant echinoderms, crinoids occupy key phylogenetic position explore evolutionary history of whole phylum. However, their development remains understudied compared that echinoderms. Therefore, aim here was establish Mediterranean feather star ( Antedon mediterranea ) as an experimental system for developmental biology. We first set up method culturing embryos in vitro and defined standardized staging this species. then optimized protocols characterize morphological molecular main structures Focusing on nervous system, we showed larval apical organ includes serotonergic, GABAergic glutamatergic neurons, which develop within conserved anterior signature. described composition early post-metamorphic revealed it has These results further our knowledge crinoid provide new techniques investigate embryogenesis. This will pave way inclusion comparative studies addressing origin echinoderm plan diversification deuterostomes.

Language: Английский

Citations

2