The seminal odorant binding protein Obp56g is required for mating plug formation and male fertility inDrosophila melanogaster DOI Creative Commons
Nora C. Brown, Benjamin Gordon, Caitlin E. McDonough-Goldstein

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 7, 2023

Abstract In Drosophila melanogaster and other insects, the seminal fluid proteins (SFPs) male sex pheromones that enter female with sperm during mating are essential for fertility induce profound post-mating effects on physiology behavior. The SFPs in D. taxa include several members of large gene family known as odorant binding (Obps). Previous work has shown some Obp genes highly expressed antennae can mediate behavioral responses to odorants, potentially by carrying these molecules receptors. These observations have led hypothesis Obps might act molecular carriers or compounds important ejaculate, though functional evidence any species is lacking. Here, we used RNAi CRISPR/Cas9 generated mutants test role seven response (PMR). We found Obp56g required induction PMR, whereas six had no effect when mutated individually. male’s ejaculatory bulb, an tissue reproductive tract synthesizes components plug. males lacking fail form a plug mated female’s tract, leading ejaculate loss reduced storage. also examined evolutionary history genes, studies documented rapid evolution turnover SFP across taxa. extensive lability copy number positive selection acting two Obp22a Obp51a . Comparative RNAseq data from multiple revealed shows high expression only obscura groups, conserved head all tested. Together, suggest may been co-opted function over time.

Language: Английский

The seminal odorant binding protein Obp56g is required for mating plug formation and male fertility in Drosophila melanogaster DOI Creative Commons
Nora C. Brown, Benjamin Gordon, Caitlin E. McDonough-Goldstein

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Dec. 21, 2023

In Drosophila melanogaster and other insects, the seminal fluid proteins (SFPs) male sex pheromones that enter female with sperm during mating are essential for fertility induce profound post-mating effects on physiology. The SFPs in D. taxa include several members of large gene family known as odorant binding (Obps). Work has shown some Obp genes highly expressed antennae can mediate behavioral responses to odorants, potentially by carrying these molecules receptors. These observations have led hypothesis Obps might act molecular carriers or compounds important fertility, though functional evidence any species is lacking. Here, we used genetics test role seven response (PMR). We found Obp56g required induction PMR, whereas six dispensable. males lacking fail form a plug mated female's reproductive tract, leading ejaculate loss reduced storage, likely due its expression ejaculatory bulb. also examined evolutionary history genes, studies documented rapid evolution turnover SFP across taxa. extensive lability copy number positive selection acting two Obp22a Obp51a. Comparative RNAseq data from tract multiple revealed shows high subset taxa, conserved head phylogeny. Together, suggest may been co-opted function over time.

Language: Английский

Citations

11
Drosophila female reproductive glands contribute to mating plug composition and the timing of sperm ejection DOI Creative Commons
Caitlin E. McDonough-Goldstein, Scott Pitnick, Steve Dorus

et al.

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2022, Volume and Issue: 289(1968)

Published: Feb. 2, 2022

Reproductive traits that influence female remating and competitive fertilization rapidly evolve in response to sexual selection conflict. One such trait, observed across diverse animal taxa, is the formation of a structural plug inside reproductive tract (FRT), either during or shortly after mating. In

Language: Английский

Citations

16
Females translate male mRNA transferred during mating DOI Creative Commons
Luciano M. Matzkin, Jeremy M. Bono,

Helen K. Pigage

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(8), P. 110442 - 110442

Published: July 3, 2024

Although RNA is found in the seminal fluid of diverse organisms, it unknown whether functional within females. We developed a proteomic method (VESPA, Variant Enabled SILAC Proteomic Analysis) to test hypothesis that Drosophila male translated by 67 male-derived, female-translated proteins (mdFTPs) female lower reproductive tracts, many with predicted functions relevant reproduction. Knockout experiments indicate mdFTPs play roles postmating interactions, affecting fertilization success, and formation/persistence insemination reaction mass, trait hypothesized be involved sexual conflict. These findings advance our understanding reproduction revealing mechanism molecular interactions between sexes strengthens extends influences on previously unrecognized ways. Given species carry fluid, this discovery has broad significance for mechanisms cooperation conflict during

Language: Английский

Citations

2
Fine structure of the female genital system of diving beetle Stictonectes optatus (Seidlitz, 1887) (Dytiscidae-Hydroporinae) and evidence of mating plug formation DOI Creative Commons
Romano Dallai, David Mercati, Pietro Paolo Fanciulli

et al.

Arthropod Structure & Development, Journal Year: 2023, Volume and Issue: 73, P. 101250 - 101250

Published: March 1, 2023

The general organization of the female genital system diving beetle Stictonectes optatus was studied, clarifying complex structure spermatheca and spermathecal gland. two structures adhere closely to each other, sharing a small area their cuticular epithelium. A long duct connects bursa copulatrix spermatheca, where sperm are stored. reach common oviduct, egg fertilization occurs, via duct. gland cells have extracellular cisterns secretions Thin ducts composed duct-forming transport these apical region into lumen. Soon after mating, is almost completely occupied by plug secreted male accessory glands. epithelium seem contribute formation. Later this becomes large spherical, obstructing copulatrix.

Language: Английский

Citations

4
Proteomic diversification of spermatostyles among six species of whirligig beetles DOI Creative Commons
R. Antonio Gómez, Romano Dallai, Dylan J. Sims‐West

et al.

Molecular Reproduction and Development, Journal Year: 2024, Volume and Issue: 91(5)

Published: May 1, 2024

Abstract Seminal fluid protein composition is complex and commonly assumed to be rapidly divergent due functional interactions with both sperm the female reproductive tract (FRT), of which evolve rapidly. In addition sperm, seminal may contain structures, such as mating plugs spermatophores. Here, we investigate evolutionary diversification a lesser‐known ejaculate structure: spermatostyle, has independently arisen in several families beetles true bugs. We characterized spermatostyle proteome, FRT morphology, six species whirligig (family Gyrinidae). Spermatostyles were enriched for proteolytic enzymes, assays confirmed they possess activity. Sperm‐leucylaminopeptidases (S‐LAPs) particularly abundant, their localization spermatostyles was by immunohistochemistry. Although there evidence conservation proteomes across species, phylogenetic regressions suggest covariation between morphology FRTs. postulate that S‐LAPs (and other proteases) have evolved novel structural role discuss adaptations delivering male‐derived materials females.

Language: Английский

Citations

1
Male Reproductive Glands and Their Secretions in Insects DOI
Ben R. Hopkins, Sarah E. Allen, Frank W. Avila

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

1
Transcriptional programs are activated and microRNAs are repressed within minutes after mating in the Drosophila melanogaster female reproductive tract DOI Creative Commons
Sofie Y. N. Delbare,

Asha M. Jain,

Andrew G. Clark

et al.

BMC Genomics, Journal Year: 2023, Volume and Issue: 24(1)

Published: June 27, 2023

The female reproductive tract is exposed directly to the male's ejaculate, making it a hotspot for mating-induced responses. In Drosophila melanogaster, changes in are essential optimize fertility. Many occur within minutes after mating, but such early timepoints absent from published RNA-seq studies. We measured transcript abundances using and microRNA-seq of tracts unmated mated females collected at 10-15 min post-mating. further investigated whether transcriptome influenced by inhibiting BMPs secondary cells, condition that depletes exosomes ejaculate.

Language: Английский

Citations

3
Genotype-by-environment interactions influence the composition of the Drosophila seminal proteome DOI Creative Commons
Valérian Zeender, Sibylle Pfammatter, Bernd Roschitzki

et al.

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2023, Volume and Issue: 290(2006)

Published: Sept. 13, 2023

Ejaculate proteins are key mediators of post-mating sexual selection and conflict, as they can influence both male fertilization success female reproductive physiology. However, the extent sources genetic variation condition dependence ejaculate proteome largely unknown. Such knowledge could reveal targets mechanisms inform about relative costs allocation different components, each with its own potential fitness consequences. Here, we used liquid chromatography coupled tandem mass spectrometry to characterize whole-ejaculate protein composition across 12 isogenic lines Drosophila melanogaster that were reared on a high- or low-quality diet. We discovered new in transferred inferred their origin system. further found was mainly determined by genotype identity genotype-specific responses larval diet, no clear overall diet effect. Nutrient restriction increased proteolytic activity shifted balance between function RNA metabolism. Our results open avenues for exploring intricate role genotypes environment shaping composition, studying functional dynamics evolutionary multivariate complexity.

Language: Английский

Citations

3
Females translate male mRNA transferred during mating DOI Creative Commons
Luciano M. Matzkin, Jeremy M. Bono,

Helen K. Pigage

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 23, 2023

Although RNA is found in the seminal fluid of diverse organisms, it unknown whether this functional within females. Here, we develop an experimental proteomic method called VESPA (Variant Enabled SILAC Proteomic Analysis) to test hypothesis that

Language: Английский

Citations

2
On how to identify a seminal fluid protein: A commentary on Hurtado et al. DOI
Stuart Wigby, Nora C. Brown, Irem Sepil

et al.

Insect Molecular Biology, Journal Year: 2022, Volume and Issue: 31(5), P. 533 - 536

Published: Aug. 17, 2022

Seminal fluid proteins (Sfps) have striking effects on the behaviour and physiology of females in many insects. Some Drosophila melanogaster Sfps are not highly or exclusively expressed accessory glands, but derive from, additionally other male reproductive tissues. The full suite includes transferred from all tissues, regardless expression level presence a signal peptide.

Language: Английский

Citations

3