Performance characteristics allow for confinement of a CRISPR toxin-antidote gene drive designed for population suppression DOI Creative Commons
Shijie Zhang, Jackson Champer

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Dec. 15, 2022

Abstract Gene drives alleles that can bias their own inheritance are a promising way to engineer populations for control of disease vectors, invasive species, and agricultural pests. Recent advancements in the field have yielded successful examples powerful suppression type confined modification drives, but developing has proven more difficult. This is because necessary power strong often incompatible with characteristics needed drive confinement. However, one CRISPR toxin-antidote may be enough confined, TADE (Toxin-Antidote Dominant Embryo) drive. By disrupting haplolethal target gene haplosufficient female fertility gene, this quickly eliminates wild-type eventually induces population suppression. It been shown perform effectively panmictic populations. confinement spatial scenarios substantially different. Here, we use reaction-diffusion model assess performance continuous space. We measure wave advance speed while varying several parameters find moderate fitness costs or embryo cutting (from maternally deposited nuclease) eliminate drive’s ability form advance. release size required propagate, finally, investigate migration corridor scenarios. Depending on dispersal, it possible suppress then persist without invading second population. prevents re-invasion by wild-type, which particularly desirable outcome some Thus, even imperfect variants excellent candidates

Language: Английский

Adversarial interspecies relationships facilitate population suppression by gene drive in spatially explicit models DOI
Yiran Liu, WeiJian Teo, Haochen Yang

et al.

Ecology Letters, Journal Year: 2023, Volume and Issue: 26(7), P. 1174 - 1185

Published: May 10, 2023

Suppression gene drives bias their inheritance to spread through a population, potentially eliminating it when they reach high frequency. CRISPR homing suppression have already seen success in the laboratory, but several models predict that may be elusive population with realistic spatial structure due extinction-recolonization cycles. Here, we extend our continuous space framework include two competing species or predator-prey pairs. We find both general and mosquito-specific models, predators can facilitate drive-based suppression, albeit at cost of an increased rate drive loss outcomes. These results are robust mosquito seasonal fluctuations. Our study illustrates difficulty predicting outcomes complex ecosystems. However, promising for prospects less powerful successfully eliminate target other pest populations.

Language: Английский

Citations

16
Population suppression by release of insects carrying a dominant sterile homing gene drive targeting doublesex in Drosophila DOI Creative Commons
Weizhe Chen,

Jialiang Guo,

Yiran Liu

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 14, 2024

Language: Английский

Citations

7
The potential of gene drives in malaria vector species to control malaria in African environments DOI Creative Commons
Penelope A. Hancock, Ace North, Adrian W. Leach

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 17, 2024

Gene drives are a promising means of malaria control with the potential to cause sustained reductions in transmission. In real environments, however, their impacts will depend on local ecological and epidemiological factors. We develop data-driven model investigate gene that causes vector population suppression. simulate drive releases sixteen ~ 12,000 km

Language: Английский

Citations

6
Experimental demonstration of tethered gene drive systems for confined population modification or suppression DOI Creative Commons
Matthew Metzloff, Emily Yang, Sumit Dhole

et al.

BMC Biology, Journal Year: 2022, Volume and Issue: 20(1)

Published: May 24, 2022

Homing gene drives hold great promise for the genetic control of natural populations. However, current homing systems are capable spreading uncontrollably between populations connected by even marginal levels migration. This could represent a substantial sociopolitical barrier to testing or deployment such and may generally be undesirable when objective is only local population control, as suppression an invasive species outside its native range. Tethered drive systems, in which locally confined provides CRISPR nuclease needed drive, provide solution this problem, offering power confinement supporting drive.

Language: Английский

Citations

26
Slow and steady wins the race: Spatial and stochastic processes and the failure of suppression gene drives DOI
Jefferson Paril, Ben L. Phillips

Molecular Ecology, Journal Year: 2022, Volume and Issue: 31(17), P. 4451 - 4464

Published: July 5, 2022

Gene drives that skew sex ratios offer a new management tool to suppress or eradicate pest populations. Early models and empirical work suggest these suppression can completely well-mixed populations, but incorporate stochasticity space (i.e. drift recolonization events) often result in loss failure of the drive. We developed stochastic model examine processes simple one-dimensional space. This allows us map events outcomes emerged how properties drive's wave invasion affect outcomes. Our simulations, across biologically realistic section parameter space, drive might be common outcome spatially explicit, systems, appear mediate Surprisingly, would considered fittest an aspatial were strongly associated with spatial setting. The cause relatively fast moving, narrow waves have high chance being penetrated by wild-types (WTs) leading WT recolonization, failure. results also show rates dispersal reduce because get disproportionately wider than as increase. Overall, wide, slow-moving much less prone point complexity inherent using genetic system effect demographic speak clear need for ecological evolutionary modelling inform design process.

Language: Английский

Citations

25
Anopheles homing suppression drive candidates exhibit unexpected performance differences in simulations with spatial structure DOI Creative Commons
Samuel E. Champer, Isabel K. Kim, Andrew G. Clark

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Oct. 14, 2022

Recent experiments have produced several Anopheles gambiae homing gene drives that disrupt female fertility genes, thereby eventually inducing population collapse. Such may be highly effective tools to combat malaria. One such drive, based on the zpg promoter driving CRISPR/Cas9, was able eliminate a cage of mosquitoes. A second version, purportedly improved upon first by incorporating an X-shredder element (which biases inheritance towards male offspring), similarly successful. Here, we analyze experimental data from each these extract their characteristics and performance parameters compare previous interpretations performance. We assess suppression drive within individual-based simulation framework models mosquito dynamics in continuous space. find combined homing/X-shredder is actually less at context our model. In particular, often fails completely suppress population, instead resulting unstable equilibrium between wild-type alleles. By contrast, otherwise similar nos prove more promising candidates for future development than originally thought.

Language: Английский

Citations

25
Whitefly–Plant Interactions: An Integrated Molecular Perspective DOI Creative Commons
Shai Morin, Peter W. Atkinson, Linda L. Walling

et al.

Annual Review of Entomology, Journal Year: 2023, Volume and Issue: 69(1), P. 503 - 525

Published: Oct. 10, 2023

The rapid advances in available transcriptomic and genomic data our understanding of the physiology biochemistry whitefly-plant interactions have allowed us to gain new significant insights into biology whiteflies their successful adaptation host plants. In this review, we provide a comprehensive overview mechanisms that evolved overcome challenges feeding on phloem sap. We also highlight evolution functions gene families involved perception, evaluation, manipulation; primary metabolism; metabolite detoxification. discuss emerging themes plant immunity whiteflies, focusing whitefly effectors sites action defense-signaling pathways. conclude with discussion genetic manipulation potential they hold for exploring between plants, as well development novel strategies control whiteflies.

Language: Английский

Citations

13
Population suppression with dominant female-lethal alleles is boosted by homing gene drive DOI Creative Commons
Jinyu Zhu,

Jingheng Chen,

Yiran Liu

et al.

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 11, 2024

Language: Английский

Citations

4
Genome Editing in Hymenoptera DOI Creative Commons
Hamish A. Salvesen, Peter K. Dearden

Insect Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: 180, P. 104300 - 104300

Published: March 11, 2025

The application of genome editing tools in Hymenoptera has transformative potential for functional genetics and understanding their unique biology. comprise one the most diverse Orders animals, development methods efficiently creating precise modifications could have applications conservation, pest management agriculture. To date, sex determination, DNA methylation, taste smell sensory systems as well phenotypic markers been selected gene investigations. From these data, insights into eusociality, nature haplodiploidy complex communication that possess provided an evolutionary history led them to become so successful. Insights from analyses supported by ever-improving suite CRIPSR further expansion will allow more specific biological hypotheses be tested beyond lab. Looking ahead, Hymenopteran modifying biocontrol agents agricultural pests use managing invasive species through technologies such drives. This review provides accessibility information regarding status editing, intending support considered CRISPR novel innovation refinement which it already achieved.

Language: Английский

Citations

0
Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study DOI Creative Commons
Jiahe Li, Jackson Champer

PLoS Genetics, Journal Year: 2023, Volume and Issue: 19(1), P. e1010591 - e1010591

Published: Jan. 23, 2023

Wolbachia are maternally-inherited bacteria, which can spread rapidly in populations by manipulating reproduction. cifA and cifB genes found phage that responsible for cytoplasmic incompatibility, the most common type of reproductive interference. In this phenomenon, no viable offspring produced when a male with both (or just some systems) mates female lacking cifA. Utilizing feature, we propose new types toxin-antidote gene drives be constructed only these two an insect genome, instead whole bacteria. By using mathematical simulation models, drive containing together creates confined moderate to high introduction threshold. When introduced separately, they act as self-limiting drive. We observed performance is substantially influenced various ecological parameters characteristics. Extending our models continuous space, individual release distribution has critical impact on persistence. Our results suggest based transgenes safe flexible candidates genetic modification populations.

Language: Английский

Citations

9