Nature, Journal Year: 2022, Volume and Issue: unknown
Published: Oct. 11, 2022
Language: Английский
eLife, Journal Year: 2024, Volume and Issue: 12
Published: June 4, 2024
Animals are adapted to their natural habitats and lifestyles. Their brains perceive the external world via sensory systems, compute information together with that of internal states autonomous activity, generate appropriate behavioral outputs. However, how do these processes evolve across evolution? Here, focusing on sense olfaction, we have studied evolution in olfactory sensitivity, preferences, responses six different food-related amino acid odors two eco-morphs fish Astyanax mexicanus . To this end, developed a high-throughput setup pipeline quantitative qualitative behavior analysis, tested 489 six-week-old larvae. The blind, dark-adapted morphs species showed markedly distinct basal swimming patterns odors, higher strong preference for alanine, as compared river-dwelling eyed conspecifics. In addition, discovered an individual ‘swimming personality’, personality influences capability respond efficiently find source. Importantly, traits favored significant were surface cavefish. Moreover, displayed by second-generation cave × F2 hybrids suggested olfactory-driven sensitivity is genetic trait. Our findings show processing has rapidly evolved cavefish at several levels: detection threshold, odor preference, foraging strategy. Cavefish therefore outstanding model understand genetic, molecular, neurophysiological basis specialization response environmental change.
Language: Английский
Citations
4
Proceedings of the Royal Society B Biological Sciences, Journal Year: 2022, Volume and Issue: 289(1984)
Published: Oct. 12, 2022
The genus Sinocyclocheilus, comprising a large radiation of freshwater cavefishes, are well known for their presence regressive features (e.g. variable eye reduction). Fewer constructive known, such as the expansion lateral line system (LLS), which is involved in detecting water movements. precise relationship between LLS and cave adaptation not understood. Here, we examine morphology LLS-mediated behaviour Sinocyclocheilus species characterized by broad variation size, habitat geographical distribution. Using live-staining techniques automated behavioural analyses, examined 26 quantified neuromast organ number, density asymmetry within phylogenetic context. We then how these morphological may relate to wall-following, an established cave-associated mediated line. show that most demonstrated laterality (i.e. asymmetry) organs on head, often biased right. also found wall-following was distinctive, particularly among eyeless species. Patterns appear correlate with degree loss, This work reveals evolution convergent across distant cavefish taxa mediate asymmetric enable survival stark subterranean microenvironments.
Language: Английский
Citations
11
Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(6)
Published: June 1, 2024
Abstract With 75 known species, the freshwater fish genus Sinocyclocheilus is largest cavefish radiation in world and shows multiple adaptations for cave‐dwelling (stygomorphic adaptations), which include a range of traits such as eye degeneration (normal‐eyed, micro‐eyed eyeless), depigmentation skin, some presence “horns”. Their behavioural to subterranean environments, however, are poorly understood. Wall‐following (WF) behaviour, where an organism remains close contact with boundary demarcating its habitat when dark, peculiar behaviour observed wide animals enhanced cave dwellers. Hence, we hypothesise that wall‐following also present , possibly eyeless species compared bearing (normal‐/micro‐eyed species). Using 13 representative morphs, designed series assays, based on pre‐existing methods Astyanax mexicanus experiments, examine under three conditions. Our results indicate exhibit significantly intensities WF normal‐eyed forms demonstrating intermediate distance. mtDNA dated phylogeny (chronogram four clades A–D), traced degree these outline common patterns. We show intensity higher dominated by free‐living species. found highly sensitive vibrations, whereas least sensitive. Since presented all given fishes evolved late Miocene, identify this being ancestral enhancement related occupation. Results from diversification‐scale study suggest may be convergent trait across stygomorphic lineages.
Language: Английский
Citations
1
Environmental Science and Pollution Research, Journal Year: 2023, Volume and Issue: 30(39), P. 90489 - 90499
Published: July 21, 2023
Language: Английский
Citations
3
Frontiers in Ecology and Evolution, Journal Year: 2023, Volume and Issue: 11
Published: Oct. 25, 2023
Introduction With 78 species, Sinocyclocheilus cavefish constitute the largest radiation in world. They exhibit remarkable morphological diversity across three habitat types: surface (surface morphs, normal-eyed, variably colored), exclusively-cave-dwelling (stygobitic eyeless, depigmented), and intermediate between cave (stygophilic micro-eyed, partially depigmented). Distinctive traits of include variations eye skin conditions associated with their habitat, despite role sensing environmental changes, its correlates are less understood, compared to well-studied conditions. Methods Here, we analyzed correlation morphology utilizing transcriptomics-based methods. We generated RNA-sequencing data for nine species integrated those existing from five additional species. These 14 represent primary clades major habitats these cavefish. Result identified 7,374 orthologous genes transcriptome data. Using a comparative transcriptomics approach, 1,348 differentially expressed (DEGs) were morphotypes. GO KEGG enrichment analyses suggested that mainly involved energy metabolism, immunity oxidative stress-related functions. Genes related immune, apoptotic, necrotic functions through positive selection analysis genes. The maximum likelihood phylogenetic tree, based on 1,369, single-copy was largely concordant currently established RAD-seq mt-DNA phylogenies. Species higher dependence present lighter coloration, fewer dark blotches, diminished scale coverage. also found differences gene expression effects may have contributed degradation color scales. Discussion Our study highlights significance shaping pigmentation variation, while offering insights into molecular mechanisms driving habitat-specific adaptations . findings underscore transcriptional variation adapting diverse environments contribute future studies evolution ecology
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Feb. 15, 2024
Abstract Studying neural mechanisms in complementary model organisms from different ecological niches the same animal class can leverage comparative brain analysis at cellular level. To advance such a direction, we developed unified atlas platform and specialized tools that allowed us to quantitatively compare structures two teleost larvae, medaka ( Oryzias latipes ) zebrafish Danio rerio ). Leveraging this quantitative approach found most regions are similar but some subpopulations unique each species. Specifically, confirmed existence of clear dorsal pallial region telencephalon lacking zebrafish. Further, our allows for extraction differentially expressed genes both species, comparison activity resolution. The web-based interactive nature will facilitate community’s research its easy extensibility encourage contributions continuous expansion.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: July 17, 2023
ABSTRACT With 78 species, Sinocyclocheilus cavefish constitute the largest radiation in world. They exhibit remarkable morphological diversity across three habitat types: surface (Surface morphs, Normal-eyed, variably colored), exclusively-cave-dwelling (Stygobitic Eyeless, depigmented), and intermediate between cave (Stygophilic Micro-eyed, partially depigmented). Distinctive traits of include variations eye skin conditions associated with their habitat, despite role sensing environmental changes, its correlates are less understood, compared to well-studied conditions. Here, we analyzed correlation morphology utilizing transcriptomics-based methods. We generated RNA-sequencing data for nine species integrated those existing from five additional species. These 14 represent primary clades major habitats these cavefish. Data on color scale were 7374 orthologous genes identified. Using a comparative transcriptomics approach, identified 1,348 differentially expressed (DEGs) morphotypes. GO KEGG enrichment analyses suggest that have evolved different strategies energy metabolism, immunity, oxidative stress habitats. also found 329 positive selection (PSGs) mainly involved apoptosis, necrosis, indicating potential adaptations The maximum likelihood phylogenetic tree, based 1369 single-copy was largely concordant currently established RAD-seq mt-DNA phylogenies, but few exceptions. Species higher dependence present lighter coloration, fewer dark blotches, diminished coverage. PCA cluster analysis suggested cave-dwelling characterized by absence black similar expression patterns, convergence adaptation. Variations tyrosine metabolism may explain pigmentation differences among diverse Our study highlights significance shaping variation, while offering insights into molecular mechanisms driving habitat-specific . findings underscore transcriptional variation adapting environments contribute future studies evolution ecology
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: Sept. 1, 2023
Abstract With 75 known species, the freshwater-fish genus Sinocyclocheilus is largest cavefish radiation in world, emerging as a model system for evolutionary studies. They show multiple adaptations cave dwelling (stygomorphic adaptations), which include range of traits such eye degeneration (Normal-eyed, Micro-eyed and Eyeless), depigmentation skin, some presence “horns”. Their behavioural to subterranean environments, however, are poorly understood. Wall-following (WF) behaviour, where an organism remains close contact with boundary demarcating its habitat when dark, peculiar behaviour observed wide animals enhanced dwellers. Hence, we hypothesize wall-following be present also , possibly Eyeless species compared visual cues (Normal / species). Using 13 representative eye-morphs, designed series assays, based on pre-existing methods Astyanax mexicanus experiments, examine under three stimulation conditions. Our results indicate that eyeless exhibit significantly levels WF Normal-eyed forms demonstrating intermediate levels. mtDNA dated phylogeny (chronogram four clades A – D), traced degree these outline common patterns. We intensity high (B & C) free-living (A D). Experiments revealed highly sensitive vibrations, whereas normal-eyed least sensitive. Since all given fishes evolved late Miocene, identify this being ancestral enhancement related occupation. from diversification-scale study suggest may convergent trait across stygomorphic lineages. Significance statement freshwater cavefish, life, including degeneration, skin depigmentation, horn-like structures, well-known, but their remain understudied. Here focus phenomenon, called “wall-following,” fish stay walls absence light. hypothesized would more pronounced species. selected diversity types, different Results were intriguing; exhibited heightened sighted counterparts, small-eyed falling between. Researchers mapped phylogenetic tree, discovering pattern: cave-dwelling showed stronger than ones. prevalent and, history genus, considered intensified adaptation. These findings contribute our understanding evolution, suggesting shared among diverse
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: Oct. 2, 2023
Abstract Animals are adapted to their natural habitats and lifestyles. Their brains perceive the external world via sensory systems, compute information together with that of internal states autonomous activity, generate appropriate behavioral outputs. However, how do these processes evolve across evolution? Here, focusing on sense olfaction, we have studied evolution in olfactory sensitivity, preferences responses six different food-related amino acid odors two eco-morphs fish Astyanax mexicanus . To this end, developed a high-throughput setup pipeline quantitative qualitative behavior analysis, tested 489 six-week-old larvae. The blind, dark-adapted morphs species showed markedly distinct basal swimming patterns odors, higher sensitivity strong preference for alanine, as compared river-dwelling eyed conspecifics. In addition, discovered an individual “swimming personality”, personality influences capability respond efficiently find source. Importantly, traits favored significant were surface cavefish. Moreover, displayed by second-generation cave x F2 hybrids suggested olfactory-driven is genetic trait. Our findings show processing has rapidly evolved cavefish at several levels: detection threshold, odor preference, foraging strategy. Cavefish therefore outstanding model understand genetic, molecular neurophysiological basis specialization response environmental change.
Language: Английский
Citations
0
eLife, Journal Year: 2023, Volume and Issue: 12
Published: Dec. 19, 2023
Animals are adapted to their natural habitats and lifestyles. Their brains perceive the external world via sensory systems, compute information together with that of internal states autonomous activity, generate appropriate behavioral outputs. However, how do these processes evolve across evolution? Here, focusing on sense olfaction, we have studied evolution in olfactory sensitivity, preferences, responses six different food-related amino acid odors two eco-morphs fish Astyanax mexicanus . To this end, developed a high-throughput setup pipeline quantitative qualitative behavior analysis, tested 489 six-week-old larvae. The blind, dark-adapted morphs species showed markedly distinct basal swimming patterns odors, higher strong preference for alanine, as compared river-dwelling eyed conspecifics. In addition, discovered an individual ‘swimming personality’, personality influences capability respond efficiently find source. Importantly, traits favored significant were surface cavefish. Moreover, displayed by second-generation cave × F2 hybrids suggested olfactory-driven sensitivity is genetic trait. Our findings show processing has rapidly evolved cavefish at several levels: detection threshold, odor preference, foraging strategy. Cavefish therefore outstanding model understand genetic, molecular, neurophysiological basis specialization response environmental change.
Language: Английский
Citations
0