Eurasian spruce bark beetle detects lanierone using a highly expressed specialist odorant receptor, present in several functional sensillum types DOI Creative Commons
Jothi Kumar Yuvaraj, Dineshkumar Kandasamy,

Rebecca E. Roberts

et al.

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 20, 2024

Abstract Background Insects detect odours using odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs) the antennae. Ecologically important are often detected by selective and abundant OSNs; hence, ORs with high antennal expression. However, little is known about function of highly beetles, since few have been functionally characterized. Here, we characterized most OR (ItypOR36) bark beetle Ips typographus L. (Coleoptera, Curculionidae, Scolytinae), a major pest spruce. We hypothesized that this would compound to fitness, such as pheromone component. next investigated distribution single sensillum recordings (SSR) situ hybridization, followed field- laboratory experiments evaluate behavioural effects discovered ligand. Results ItypOR36 HEK293 cells challenged it 64 ecologically relevant odours. The responded exclusively monoterpene-derived ketone lanierone sensitivity. Lanierone used chemical communication North American species, but has never shown be produced I. , nor studied relation species’ physiology. Single revealed novel lanierone-responsive OSN class same specific response ItypOR36. Strikingly, these OSNs were co-localized sensilla together seven different previously described classes. Field low release rates inhibited attraction traps baited aggregation pheromone, strongest on males. Female beetles attracted walking bioassays. Conclusions Our study highlights importance so-called ‘reverse ecology’ approach identify semiochemicals for insect species. discovery co-localization pattern involving suggests organizational differences peripheral sense between orders. show elicits responses two sexes, which also depend whether or flying field. Unravelling source natural environment required understand context-dependent behaviours.

Language: Английский

Females smell differently: characteristics and significance of the most common olfactory sensilla of female silkmoths DOI Creative Commons

Elisa Schuh,

Sina Cassau,

Ahmed Ismaieel

et al.

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 291(2015)

Published: Jan. 17, 2024

In the silkmoth Bombyx mori , role of male sensilla trichodea in pheromone detection is well established. Here we study corresponding female sensilla, which contain two olfactory sensory neurons (OSNs) and come lengths, each representing a single physiological type. Only OSNs medium trichoids respond to scent mulberry, silkworm's exclusive host plant, are more sensitive mated females, suggesting oviposition. long trichoids, one OSN tuned (+)-linalool other benzaldehyde isovaleric acid, both odours emitted by silkworm faeces. While significance remains unclear, acid repels females may therefore play avoiding crowded oviposition sites. When examined underlying molecular components found non-canonical co-expression Ir8a co-receptor for responses, ORco odorant receptors, unexpected expression specific receptor trichoid sensillum types. addition elucidating function our results suggest that some accepted organizational principles insect system not apply predominant on antenna B. .

Language: Английский

Citations

6
Olfactory receptor coexpression and co-option in the dengue mosquito DOI Creative Commons
Elisha David Adavi, Vitor L dos Anjos,

Summer Kotb

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 22, 2024

The olfactory sensory neurons of vinegar flies and mice tend to express a single ligand-specific receptor. While this ′one neuron-one receptor′ motif has long been expected apply broadly across insects, recent evidence suggests it may not extend mosquitoes. We sequenced analyzed the transcriptomes 46,000 from antennae dengue mosquito Aedes aegypti resolve all olfactory, thermosensory, hygrosensory neuron subtypes identify receptors expressed therein. find that half coexpress multiple receptors. However, coexpression occurs almost exclusively among genes same family—among odorant (ORs) or ionotropic (IRs). Coexpression ORs with IRs is exceedingly rare. Many coexpressed are duplicates. In other cases, recruitment co-option by placed these together in cells distant paralogs. Close examination data Drosophila reveal rare cases both phenomena, indicating systems two species fundamentally different, but instead fall at different locations along continuum likely encompass diverse insects.

Language: Английский

Citations

6
Impact of plant monoterpenes on insect pest management and insect-associated microbes DOI Creative Commons
Muhammad Qasim, Waqar Islam, Muhammad Rizwan

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(20), P. e39120 - e39120

Published: Oct. 1, 2024

The fight against insect pests primarily relies on the utilization of synthetic insecticides. However, improper application these chemicals can lead to detrimental effects both environment and human health, as well foster development resistance. Consequently, novel strategies must be implemented address challenges stemming from prolonged use insecticides in agricultural public health environments. Certain involve combination crop protectants, which not only enhance insecticidal effectiveness but also reduce rates. Plant-based natural products emerge promising alternatives for management. Monoterpenes, are abundant plant compounds produced through activation various enzymes, have attracted significant attention their control. Notably, they prolific fragrance-producing plants. This review explores defense, insecticidal, antimicrobial characteristics monoterpenes pests, shedding light potential modes action possibilities commercialization. Emphasizing role targeted environmentally safer, highlights practical viability within integrated pest management programs.

Language: Английский

Citations

5
The conserved IR75 subfamily mediates carboxylic acid detection in insects of public health and agricultural importance DOI Creative Commons

Matthew Cooke,

Michael Solomon Chembars,

R. Jason Pitts

et al.

Journal of Insect Science, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 1, 2025

Insects perceive and respond to carboxylic acids (CAs), amines, aldehydes primarily via conserved ionotropic receptors (IRs). These form the basis for a second olfactory system distinct from well-characterized odorant receptors. Neurons expressing IRs are housed in dedicated sensilla innervate glomeruli, separate those innervated by receptor neurons. The IR8a co-receptor is highly across insect orders and, together with tuning receptors, detects CAs. conservation of genes anatomical separation neural pathways underscore importance these compound classes CAs, specifically chemical ecology. We provide summary acid detection insects, focusing on dipteran lepidopteran species significance public health agriculture. An overview behavior toward CAs provided, as well comprehensive update function insects. Phylogenetic analysis publicly available genome databases reveals several that encode express homologs previously deorphanized highlighting avenues future research.

Language: Английский

Citations

0
Full body sensillar diversity of pumpkin fruit fly, Bactrocera tau (Walker) (Diptera: Tephritidae) DOI

Alka Rana,

Dixit Sharma, Himanshu Thakur

et al.

Zoomorphology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 11, 2024

Language: Английский

Citations

0
Eurasian spruce bark beetle detects lanierone using a highly expressed specialist odorant receptor, present in several functional sensillum types DOI Creative Commons
Jothi Kumar Yuvaraj, Dineshkumar Kandasamy,

Rebecca E. Roberts

et al.

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 20, 2024

Abstract Background Insects detect odours using odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs) the antennae. Ecologically important are often detected by selective and abundant OSNs; hence, ORs with high antennal expression. However, little is known about function of highly beetles, since few have been functionally characterized. Here, we characterized most OR (ItypOR36) bark beetle Ips typographus L. (Coleoptera, Curculionidae, Scolytinae), a major pest spruce. We hypothesized that this would compound to fitness, such as pheromone component. next investigated distribution single sensillum recordings (SSR) situ hybridization, followed field- laboratory experiments evaluate behavioural effects discovered ligand. Results ItypOR36 HEK293 cells challenged it 64 ecologically relevant odours. The responded exclusively monoterpene-derived ketone lanierone sensitivity. Lanierone used chemical communication North American species, but has never shown be produced I. , nor studied relation species’ physiology. Single revealed novel lanierone-responsive OSN class same specific response ItypOR36. Strikingly, these OSNs were co-localized sensilla together seven different previously described classes. Field low release rates inhibited attraction traps baited aggregation pheromone, strongest on males. Female beetles attracted walking bioassays. Conclusions Our study highlights importance so-called ‘reverse ecology’ approach identify semiochemicals for insect species. discovery co-localization pattern involving suggests organizational differences peripheral sense between orders. show elicits responses two sexes, which also depend whether or flying field. Unravelling source natural environment required understand context-dependent behaviours.

Language: Английский

Citations

0