A quantitative survey of the blueberry (Vaccinium spp.) culturable nectar microbiome: variation between cultivars, locations, and farm management approaches

FEMS Microbiology Ecology, Journal Year: 2024, Volume and Issue: 100(3)

Published: Feb. 14, 2024

Microbes in floral nectar can impact both their host plants and visitors, yet little is known about the microbiome of most pollinator-dependent crops. In this study, we examined abundance composition fungi bacteria inhabiting Vaccinium spp. nectar, as well volume sugar concentrations. We compared wild V. myrsinites with two field-grown corymbosum cultivars collected from organic conventional farms. Differences traits microbiomes were identified between but not species. The cultivated also varied greatly farms, whereas management regime had only subtle effects, higher fungal populations detected under management. Nectars hexose-dominant, high cell densities correlated reduced Bacteria more common than blueberry although frequently co-occurred often would be predicted by chance. "Cosmopolitan" microbes that isolated all plants, including Rosenbergiella sp. Symmetrospora symmetrica, identified. This study provides first systematic report microbiome, which may have important implications for pollinator crop health.

Language: Английский

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Pollination Unveiled: Exploring Floral Rewards

Published: Jan. 1, 2025

Language: Английский

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In the nectar, there are answers: exploring the intersection of colored nectars and reactive oxygen species in manipulating pollinator behavior

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Nectar, a vital mediator of plant-pollinator interactions, exhibits remarkable chemical diversity beyond sugars, including reactive oxygen species and specialized metabolites such as pigments. Colored nectars, present in over 70 species, function visual signals, inhibitors microbial growth, or nutritional rewards, underscoring their ecological importance. Reactive contribute to pigment formation nectar stability, highlighting dual roles chemistry defense. Advances analytical techniques interdisciplinary research have highlighted the complex interplay between composition, pollinator behavior, communities, emphasizing nectar's multifaceted plant fitness ecosystem dynamics.

Language: Английский

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Polyfloral nutritional resources promote bumble bee colony development after exposure to a pesticide mixture

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 296, P. 118170 - 118170

Published: April 11, 2025

Language: Английский

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A guide to sunflowers: floral resource nutrition for bee health and key pollination syndromes

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: May 1, 2025

Sunflower, Helianthus annuus L., is a prominent global oilseed crop with rising cultivation and appeal as bee-friendly plant by providing abundant floral resources for pollinators. Mass-flowering crops can increase the availability of resources, sunflower good opportunity to relieve pollen scarcity during late summer in agricultural landscapes. Yet this should be taken caution they also provide homogeneous source nutrition. This study aimed review summarize nutritional profile pollen, nectar, bee bread, honey, while assessing their effects on survival, development, health. Furthermore, we present here general state knowledge additional pollinator syndromes that extend beyond including those influencing visual olfactory attraction. We found pollen’s quality questioned due lower protein amino acid deficiencies, its nutrient content, like nectar sugars, had large variability. Sunflower consumption showed mixed Apis mellifera Bombus species, sometimes negatively impacting development survival. However, studies have conveyed positive impact health consistently reduced infection intensity gut parasite, Crithidia bombi , species. probes question defining emphasizing need when categorizing low resource. outlines importance characteristics (sugar content volume) morphology (flower pigmentation corolla length) foraging preferences. A gap persists regarding chemistry sunflowers’ extensive volatile better understand pollination drive interactions.

Language: Английский

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Environment or genetic isolation? An atypical intestinal microbiota in the Maltese honey bee Apis mellifera spp. ruttneri

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 23, 2023

Apis mellifera evolved mainly in African, Asian, and European continents over thousands of years, leading to the selection a considerable number honey bees subspecies that have adapted various environments such as hot semi-desert zones cold temperate zones. With evolution bee subspecies, it is possible environmental conditions, food sources, microbial communities typical colonized areas shaped gut microbiota.In this study microbiota two distinct lineages (mitochondrial haplotypes) ruttneri (lineage A) ligustica carnica (both lineage C) were compared. Honey guts collected dry period respective breeding (the island Malta regions Emilia-Romagna South Tyrol Italy). Microbial DNA from was extracted amplified for V3-V4 16S rRNA gene bacteria ITS2 fungi.The analyses carried out show Maltese A distinctive when compared Italian C bees, with most abundant genera being Bartonellaceae Lactobacillaceae, respectively. Lactobacillaceae Lineage consist Apilactobacillus instead Lactobacillus Bombilactobacillus C. also harbors higher proportions Arsenophonus, Bombella, Commensalibacter, Pseudomonas C.The environment seems be main driver acquisition these marked differences microbiota. However, influence other factors host genetics, seasonality or geography may still play significant role microbiome shaping, synergy aspects.

Language: Английский

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Honey bee colonies can buffer short-term stressor effects of pollen restriction and fungicide exposure on colony development and the microbiome

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 282, P. 116723 - 116723

Published: July 17, 2024

Honey bees (Apis mellifera) have to withstand various environmental stressors alone or in combination agriculture settings. Plant protection products are applied achieve high crop yield, but residues of their active substances frequently detected bee matrices and could affect honey colonies. In addition, intensified lead resource limitation for bees. This study aimed compare the response full-sized nucleus colonies combined fungicide exposure limitation. A large-scale field was conducted simultaneously at five different locations across Germany, starting spring 2022 continuing through 2023. The formulation Pictor® Active (active ingredients boscalid pyraclostrobin) according label instructions maximum recommended rate on oil seed rape crops. Resource ensured by pollen restriction using a trap stressor responses were evaluated assessing colony development, brood core gut microbiome alterations. Furthermore, effects plant nectar assessed since inhabiting yeast beneficial pollination. We showed, that able compensate within six weeks. Nucleus exposed showed short-term with less favorable ratio reduced development May. No further impacts observed either from July until following spring. no fungicide-dependent differences found microbiomes, these not distinguishable local effects. Therefore, provision sufficient resources is important increase resilience stressors.

Language: Английский

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Impact of Nectar Composition and Nectar Yeasts on Volatile Emissions and Parasitoid Behavior

Journal of Chemical Ecology, Journal Year: 2025, Volume and Issue: 51(2)

Published: March 6, 2025

Nectar yeasts can significantly influence the scent of floral nectar and therefore foraging behavior flower-visiting insects. While these effects likely depend on chemistry yeast species, their joint impact volatile profiles associated insect responses remain poorly understood. Here, we used four synthetic types varying in sugar amino acid concentration two specialist (Metschnikowia gruessii Metschnikowia reukaufii) to investigate how composition species affect olfactory generalist aphid parasitoid Aphidius ervi. Olfactometer assays showed that A. ervi females preferred fermented nectars with high acid-low content (HL) low acid-high (LH) content, regardless being by M. or reukaufii, over non-inoculated nectars. This effect was not observed for (LL) (HH) content. Moreover, LL became even repellent parasitoids. GC-MS analysis organic compounds (VOCs) revealed VOC depended type (i.e., chemical composition), interaction. Whereas propyl acetate, isobutyl styrene, α-guaiene pentyl-octanoate were LH nectars, ethyl acetate E-methyl isoeugenol mainly HL suggesting possible involvement attraction In contrast, isopropyl-hexadecanoate non-attractive Altogether, our results indicate has a strong when subsequently behavior.

Language: Английский

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The Floral Microbiome and Its Management in Agroecosystems: A Perspective

Journal of Agricultural and Food Chemistry, Journal Year: 2022, Volume and Issue: 70(32), P. 9819 - 9825

Published: Aug. 2, 2022

Disease management is critical to ensuring healthy crop yields and often targeted at flowers because of their susceptibility pathogens direct link reproduction. Many disease strategies are unsustainable however the potential for evolve resistance, or nontarget effects on beneficial insects. Manipulating floral microbiome holds some promise as a sustainable alternative chemical means control. In this perspective, we discuss current state research concerning assembly in agroecosystems well future directions aimed improving sustainability control insect-mediated ecosystem services.

Language: Английский

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Tiny but mighty? Overview of a decade of research on nectar bacteria

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

An emerging focus of research at the intersection botany, zoology, and microbiology is study floral nectar as a microbial habitat, referred to microbiome, which can alter plant-pollinator interactions. Studies on these communities have primarily focused yeasts, it was only about decade ago that bacteria began be studied widespread inhabitants nectar. This review aims give an overview current knowledge bacteria, with emphasis evolutionary origin, dispersal mode, effects chemistry plant-animal interactions, community assembly, agricultural applications, their use model systems in ecological research. We further outline gaps our understanding significance microorganisms, response environmental changes, potential cascading effects.

Language: Английский

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