Structural features of sensory two component systems: a synthetic biology perspective DOI
Marcos Nieves, Alejandro Buschiazzo, Felipe Trajtenberg

et al.

Biochemical Journal, Journal Year: 2023, Volume and Issue: 480(2), P. 127 - 140

Published: Jan. 23, 2023

All living organisms include a set of signaling devices that confer the ability to dynamically perceive and adapt fluctuating environment. Two-component systems are part this sensory machinery regulates execution different genetic and/or biochemical programs in response specific physical or chemical signals. In last two decades, there has been tremendous progress our molecular understanding on how signals detected, allosteric mechanisms control intramolecular information transmission specificity determinants guarantee correct wiring. is starting be exploited development new synthetic networks. Connecting multiple players, analogous programming lines code, can provide resources build sophisticated biocomputing systems. The Synthetic Biology field revolutionize several scientific fields, such as biomedicine agriculture, propelling solutions. Expanding spectrum available nanodevices toolbox key unleash its full potential. This review aims discuss, from structural perspective, take advantage vast array sensor effector protein modules involved two-component for construction circuits.

Language: Английский

A comprehensive review of sustainable bioremediation techniques: Eco friendly solutions for waste and pollution management DOI Creative Commons

Narendra Kuppan,

Midhila Padman,

M Mahadeva

et al.

Waste Management Bulletin, Journal Year: 2024, Volume and Issue: 2(3), P. 154 - 171

Published: July 22, 2024

Bioremediation, an advanced and environmentally sustainable technology, utilizes biological microorganisms to mitigate pollution. This review combines insights from two perspectives: one focusing on the mechanisms, applications, types of bioremediation, other examining transformative potential integrating Internet Things (IoT), Artificial Intelligence (AI), biosensors in pollution management. The first perspective delves into effectiveness bioremediation decomposing detoxifying hazardous substances, emphasizing its cost-effectiveness eco-friendliness compared conventional methods. In-situ ex-situ methods are analyzed, along with intrinsic engineered techniques, phytoremediation strategies for heavy metal removal. underscores growing importance addressing industrial effluents, contaminated soils, groundwater, future advancements expected enhance efficiency applicability. From second perspective, recent IoT, AI, explored their revolutionize waste IoT facilitates real-time monitoring remote management, AI enhances data analysis predictive modelling, contribute precise pollutant detection environmental monitoring. highlights synergistic integration these technologies, presenting smart systems feedback loops adaptive capabilities. Together, technologies offer scalable solutions mitigation, marking a significant stride towards

Language: Английский

Citations

42
Pseudomonas putida KT2440: the long journey of a soil-dweller to become a synthetic biology chassis DOI
Vı́ctor de Lorenzo, Danilo Pérez‐Pantoja, Pablo I. Nikel

et al.

Journal of Bacteriology, Journal Year: 2024, Volume and Issue: 206(7)

Published: July 8, 2024

Although members of the genus

Language: Английский

Citations

18
Microbes Saving Lives and Reducing Suffering DOI Creative Commons
Kenneth N. Timmis, Zeynep Ceren Karahan, Juan L. Ramos

et al.

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 1, 2025

Language: Английский

Citations

3
Synthetic Biology for Waste Water to Energy Conversion DOI

N. Venkateswaran,

Shanmugam Senthil Kumar, G. Diwakar

et al.

Advances in bioinformatics and biomedical engineering book series, Journal Year: 2023, Volume and Issue: unknown, P. 360 - 384

Published: Oct. 2, 2023

Waste-to-energy (WtE) is a process that generates electricity and heat from waste treatment or processing into fuel sources. WtE can reduce greenhouse gas emissions, landfill waste, dependence on fossil fuels. However, it faces challenges such as high capital operational costs, environmental health impacts, public acceptance. Synthetic biology (SynBio) offer novel solutions for by enhancing efficiency, sustainability, diversity of biological processes. This chapter reviews the current state-of-the-art future prospects SynBio applications WtE, focusing integration IoT AI technologies. network physical devices, sensors, actuators collect, transmit, data over internet, while simulates human intelligence in machines. enable smart monitoring, control, optimization systems providing data-driven insights decision support stakeholders.

Language: Английский

Citations

36
Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform DOI Creative Commons
Esteban Martínez‐García, Vı́ctor de Lorenzo

Current Opinion in Biotechnology, Journal Year: 2023, Volume and Issue: 85, P. 103025 - 103025

Published: Dec. 7, 2023

The soil bacterium Pseudomonas putida, especially the KT2440 strain, is increasingly being utilized as a host for biotransformations of both industrial and environmental interest. foundations such performance include its robust redox metabolism, ability to tolerate wide range physicochemical stresses, rapid growth, versatile nonpathogenic nature, availability molecular tools advanced genetic programming. These attributes have been leveraged hosting engineered pathways production valuable chemicals or degradation/valorization pollutants. This has in turn pushed boundaries conventional enzymology toward previously unexplored reactions nature. Furthermore, modifications physical properties cells made enhance their catalytic performance. advancements establish P. putida bona fide chassis synthetic biology, on par with more traditional metabolic engineering platforms.

Language: Английский

Citations

33
Ecological complexity and the biosphere: the next 30 years DOI Creative Commons
Ricard V. Solé, Simon A. Levin

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2022, Volume and Issue: 377(1857)

Published: June 27, 2022

Global warming, habitat loss and overexploitation of limited resources are leading to alarming biodiversity declines. Ecosystems complex adaptive systems that display multiple alternative states can shift from one another in abrupt ways. Some these tipping points have been identified predicted by mathematical computational models. Moreover, scales involved potential mitigation or intervention scenarios tied particular levels complexity, cells human–environment coupled systems. In dealing with a biosphere where humans part complex, endangered ecological network, novel theoretical engineering approaches need be considered. At the centre most research efforts is biodiversity, which essential maintain community resilience ecosystem services. What done mitigate, counterbalance prevent points? Using 30-year window, we explore recent sense, preserve restore as well number proposed interventions (from afforestation bioengineering) directed mitigate reverse collapse. The year 2050 taken representative future horizon combines time scale deep changes will occur solutions might effective. This article theme issue ‘Ecological complexity biosphere: next 30 years’.

Language: Английский

Citations

36
Synthetic Biology─High Time to Deliver? DOI Creative Commons
Andrew D. Hanson, Vı́ctor de Lorenzo

ACS Synthetic Biology, Journal Year: 2023, Volume and Issue: 12(6), P. 1579 - 1582

Published: June 16, 2023

Synthetic biology (SynBio) has attracted like no other recent development the attention not only of Life Science researchers and engineers but also intellectuals, technology think-tanks, private public investors. This is largely due to its promise propel biotechnology beyond traditional realms in medicine, agriculture, environment toward new territories historically dominated by chemical manufacturing industries─but now claimed be amenable complete biologization. For this happen, it crucial for field remain true foundational engineering drive, which relies on mathematics quantitative tools construct practical solutions real-world problems. article highlights several SynBio themes that, our view, come with somewhat precarious promises that need tackled. First, must critically examine whether enough basic information available enable design or redesign life processes turn from a descriptive science into prescriptive one. Second, unlike circuit boards, cells are built soft matter possess inherent abilities mutate evolve, even without external cues. Third, cannot presented as one technical solution many grave world problems so avoid exaggerated claims hype. Finally, should pay heed sensitivities involve social growth, thus change narrative sheer domination living conversation win-win partnership.

Language: Английский

Citations

16
Running the numbers on plant synthetic biology solutions to global problems DOI Creative Commons
Kristen Van Gelder, Edmar R. Oliveira-Filho, Carlos D. Messina

et al.

Plant Science, Journal Year: 2023, Volume and Issue: 335, P. 111815 - 111815

Published: Aug. 3, 2023

Synthetic biology and metabolic engineering promise to deliver sustainable solutions global problems such as phasing out fossil fuels replacing industrial nitrogen fixation. While this is real, scale matters, so do knock-on effects of implementing solutions. Both can be estimated by 'Fermi calculations' (aka 'back-of-envelope calculations) that use uncontroversial input data plus simple arithmetic reach rough but reliable conclusions. Here, we illustrate how done informative it using two cases: oilcane (sugarcane engineered accumulate triglycerides instead sugar) a source bio-jet fuel, fixation bacteria in mucilage secreted maize aerial roots. We estimate could meet no more than about 1% today's U.S. jet fuel demand if grown on all current sugarcane land that, cane were expanded two-thirds demand, the fertilizer refinery requirements would create large carbon footprint. Conversely, aerial-root replace up 10% applied maize, 2% plant income used for growth suffice fixation, extra consumption likely reduce grain yield only slightly.

Language: Английский

Citations

11
A roadmap to understanding and anticipating microbial gene transfer in soil communities DOI
David L. Gillett, Malyn A. Selinidis, Travis R. Seamons

et al.

Microbiology and Molecular Biology Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

SUMMARY Engineered microbes are being programmed using synthetic DNA for applications in soil to overcome global challenges related climate change, energy, food security, and pollution. However, we cannot yet predict gene transfer processes assess the frequency of unintentional engineered environmental when applying biology technologies at scale. This challenge exists because complex heterogeneous characteristics soils, which contribute fitness transport cells exchange genetic material within communities. Here, describe knowledge gaps about across microbiomes. We propose strategies improve our understanding communities, highlight need benchmark performance biocontainment measures situ , discuss responsibly engaging community stakeholders. opportunities address gaps, such as creating a set standards studying diverse types measuring host range microbiomes emerging technologies. By comparing rates, range, persistence different posit that community-scale, environment-specific models can be built anticipate biotechnology risks. Such studies will enable design safer biotechnologies allow us realize benefits mitigate risks associated with release

Language: Английский

Citations

0
Engineering biology applications for environmental solutions: potential and challenges DOI Creative Commons
David J. Lea‐Smith, Francis Hassard, Frédéric Coulon

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 14, 2025

Abstract Engineering biology applies synthetic to address global environmental challenges like bioremediation, biosequestration, pollutant monitoring, and resource recovery. This perspective outlines innovations in engineering biology, its integration with other technologies (e.g., nanotechnology, IoT, AI), commercial ventures leveraging these advancements. We also discuss commercialisation scaling challenges, biosafety biosecurity considerations including biocontainment strategies, social political dimensions, governance issues that must be addressed for successful real-world implementation. Finally, we highlight future perspectives propose strategies overcome existing hurdles, aiming accelerate the adoption of solutions.

Language: Английский

Citations

0