Structural features of sensory two component systems: a synthetic biology perspective DOI
Marcos Nieves, Alejandro Buschiazzo, Felipe Trajtenberg

et al.

Biochemical Journal, Journal Year: 2023, Volume and Issue: 480(2), P. 127 - 140

Published: Jan. 23, 2023

All living organisms include a set of signaling devices that confer the ability to dynamically perceive and adapt fluctuating environment. Two-component systems are part this sensory machinery regulates execution different genetic and/or biochemical programs in response specific physical or chemical signals. In last two decades, there has been tremendous progress our molecular understanding on how signals detected, allosteric mechanisms control intramolecular information transmission specificity determinants guarantee correct wiring. is starting be exploited development new synthetic networks. Connecting multiple players, analogous programming lines code, can provide resources build sophisticated biocomputing systems. The Synthetic Biology field revolutionize several scientific fields, such as biomedicine agriculture, propelling solutions. Expanding spectrum available nanodevices toolbox key unleash its full potential. This review aims discuss, from structural perspective, take advantage vast array sensor effector protein modules involved two-component for construction circuits.

Language: Английский

Engineering Microorganisms for Cancer Immunotherapy DOI
Dingkang Liu,

Lichao Yu,

Haibo Rong

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(18)

Published: April 10, 2024

Cancer immunotherapy presents a promising approach to fight against cancer by utilizing the immune system. Recently, engineered microorganisms have emerged as potential strategy in immunotherapy. These microorganisms, including bacteria and viruses, can be designed modified using synthetic biology genetic engineering techniques target cells modulate This review delves into various microorganism-based therapies for immunotherapy, encompassing strategies enhancing efficacy while ensuring safety ethical considerations. The development of these holds immense offering innovative personalized treatments cancer.

Language: Английский

Citations

3
Taking Synthetic Biology to the Seas: From Blue Chassis Organisms to Marine Aquaforming DOI Creative Commons
Lennart Schada von Borzyskowski

ChemBioChem, Journal Year: 2023, Volume and Issue: 24(13)

Published: March 15, 2023

Abstract Oceans cover 71 % of Earth's surface and are home to hundreds thousands species, many which microbial. Knowledge about marine microbes has strongly increased in the past decades due global sampling expeditions, detailed studies on microbial ecology, physiology, biogeochemistry. However, translation this knowledge into biotechnological applications or synthetic biology approaches using been limited so far. This review highlights key examples bacteria metabolic engineering, outlines possible future work based emerging chassis organisms Vibrio natriegens Halomonas bluephagenesis . Furthermore, valorization algal polysaccharides by genetically enhanced is presented as an example opportunities challenges associated with blue biotechnology. Finally, new roles for tackling pressing challenges, including climate change pollution, discussed.

Language: Английский

Citations

8
Leveraging microalgae utilization for anthropogenic CO2 emission abatement in Malaysian urban centre: Revisiting the sustainable development goal (SDG-13) DOI
Olusegun Abayomi Olalere, Chee‐Yuen Gan, Pei-Gee Yap

et al.

Physics and Chemistry of the Earth Parts A/B/C, Journal Year: 2023, Volume and Issue: 129, P. 103356 - 103356

Published: Jan. 10, 2023

Language: Английский

Citations

7
Biodiversity as a firewall to engineered microbiomes for restoration and conservation DOI Creative Commons

Victor Maull,

Ricard V. Solé

Royal Society Open Science, Journal Year: 2024, Volume and Issue: 11(6)

Published: June 1, 2024

The possibility of abrupt transitions threatens to poise ecosystems into irreversibly degraded states. Synthetic biology has recently been proposed prevent them from crossing tipping points. However, there is little understanding the impact such intervention on resident communities. Can modification have ‘unintended consequences’, as loss species? Here, we address this problem by using a mathematical model that allows us simulate scenario explicitly. We show how indirect effect damping decay shared resources results in biodiversity increase, and last but not least, successful incorporation synthetic within ecological network very small-positive changes population size community. Furthermore, extensions implications for future restoration terraformation strategies are discussed.

Language: Английский

Citations

2
Propagation of Recombinant Genes through Complex Microbiomes with Synthetic Mini-RP4 Plasmid Vectors DOI Creative Commons
Tomás Aparicio,

Jillian Silbert,

Sherezade Cepeda

et al.

BioDesign Research, Journal Year: 2022, Volume and Issue: 2022

Published: Jan. 1, 2022

The promiscuous conjugation machinery of the Gram-negative plasmid RP4 has been reassembled in a minimized, highly transmissible vector for propagating genetically encoded traits through diverse types naturally occurring microbial communities. To this end, whole RP4-encoded transfer determinants (tra, mob genes, and origin oriT) was excised from their natural context, recreated form streamlined DNA segment borne by an autoselective replicon. resulting constructs (the pMATING series) could be self-transferred variety prokaryotic eukaryotic recipients employing such rationally designed conjugal delivery device. Insertion GFP reporter into exposed value genetic tool delivering heterologous genes to both specific mating partners complex consortia (e.g., plant/soil rhizosphere). results accredited effective functional recombinant plasmids diversity hosts. Yet inspection factors that limit interspecies scenarios uncovered type VI secretion systems as one factual barriers check otherwise high frequencies tested derivatives. We argue hereby presented programming hyperpromiscuous gene can become phenomenal asset propagation beneficial various scales environmental microbiome.

Language: Английский

Citations

9
The hydrocarbon pollution crisis: Harnessing the earth hydrocarbon‐degrading microbiome DOI Creative Commons
Robert Duran, Cristiana Cravo‐Laureau

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(7)

Published: July 1, 2024

Language: Английский

Citations

1
Construction of microbial platform chassis for CO2 utilisation DOI Creative Commons
Simona Della Valle, Weiming Tu, Wei E. Huang

et al.

Current Opinion in Systems Biology, Journal Year: 2023, Volume and Issue: 37, P. 100489 - 100489

Published: Nov. 25, 2023

To realise a circular bioeconomy, waste carbon streams, such as dioxide, can be utilised through microbial conversion to produce value-added compounds. Although some microorganisms are naturally able grow on these renewable feedstocks and generate desirable molecules, significant engineering is required develop platform chassis exhibiting attractive performance parameters for industrial-scale processes. Here, we provide brief overview of the core considerations in autotrophic bioproduction, including energy supply, addition emerging standards rewiring metabolic pathways enhance growth biosynthetic capabilities. We highlight examples successful strategies, placing emphasis recent advances capabilities both native autotrophs heterotrophs.

Language: Английский

Citations

3
Holistic view of biological nitrogen fixation and phosphorus mobilization in Azotobacter chroococcum NCIMB 8003 DOI Creative Commons

Karolina A. Biełło,

Carlos Lucena, Francisco J. López-Tenllado

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 8, 2023

Nitrogen (N) and phosphorus (P) deficiencies are two of the most agronomic problems that cause significant decrease in crop yield quality. N P chemical fertilizers widely used current agriculture, causing environmental increasing production costs. Therefore, development alternative strategies to reduce use while maintaining inputs being investigated. Although dinitrogen is an abundant gas atmosphere, it requires biological nitrogen fixation (BNF) be transformed into ammonium, a source assimilable by living organisms. This process bioenergetically expensive and, therefore, highly regulated. Factors like availability other essential elements, as phosphorus, strongly influence BNF. However, molecular mechanisms these interactions unclear. In this work, physiological characterization BNF mobilization (PM) from insoluble form (Ca 3 (PO 4 ) 2 Azotobacter chroococcum NCIMB 8003 was carried out. These processes were analyzed quantitative proteomics order detect their requirements interactions. led metabolic change beyond proteins strictly necessary carry out process, including metabolism related phosphorus. Also, changes cell mobility, heme group synthesis oxidative stress responses observed. study also revealed phosphatases seem have main role PM, exopolyphosphatase non-specific alkaline phosphatase PhoX. When both PM take place simultaneously, nitrogenous bases L -methionine affected. Thus, although interdependence still unknown, possible biotechnological applications should account indicated factors.

Language: Английский

Citations

2
Dissecting the HGT network of carbon metabolic genes in soil-borne microbiota DOI Creative Commons
Liangzhi Li, Yong-Jun Liu,

Qinzhi Xiao

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: July 7, 2023

The microbiota inhabiting soil plays a significant role in essential life-supporting element cycles. Here, we investigated the occurrence of horizontal gene transfer (HGT) and established HGT network carbon metabolic genes 764 soil-borne genomes. Our study sheds light on crucial components microbiological diversification that could have far-reaching implications understanding how these microbial communities adapt to changing environments, ultimately impacting agricultural practices. In overall microbiota, total 6,770 nodes 3,812 edges are present. Among nodes, phyla Proteobacteria, Actinobacteriota, Bacteroidota, Firmicutes predominant. Regarding specific classes, Actinobacteria, Gammaproteobacteria, Alphaproteobacteria, Bacteroidia, Actinomycetia, Betaproteobacteria, Clostridia dominant. Kyoto Encyclopedia Genes Genomes (KEGG) functional assignments glycosyltransferase (18.5%), glycolysis/gluconeogenesis (8.8%), carbohydrate-related transporter (7.9%), fatty acid biosynthesis (6.5%), benzoate degradation (3.1%) butanoate metabolism (3.0%) primarily identified. Glycosyltransferase involved cell wall biosynthesis, glycosylation, primary/secondary (with 363 entries), ranks first overwhelmingly list most frequently identified enzymes, followed by pimeloyl-ACP methyl ester carboxylesterase, alcohol dehydrogenase, 3-oxoacyl-ACP reductase. Such events mainly occur peripheral functions pathway instead core section. inter-microbe genetic traits sequences recognized, as well their involvement regulation processes organic, suggest pervasive substantial effect evolution microbes.

Language: Английский

Citations

2
A microbial evolutionary approach for a sustainable future DOI Creative Commons

Lawrence P. Wackett

Microbial Biotechnology, Journal Year: 2023, Volume and Issue: 16(10), P. 1895 - 1899

Published: Aug. 21, 2023

With the continued population increase, more sustainable use of water, land, air and chemicals is imperative. Microorganisms will need to be called upon aid in many sustainability efforts. Prokaryotes are fastest-evolving cellular life, most manipulatable via synthetic biology. Moreover, their natural diversity processing organic inorganic chemicals, survivability extreme niches, make them prime agents enlist for solving society's pressing problems.

Language: Английский

Citations

2