Type III Secretion in Chlamydia

Microbiology and Molecular Biology Reviews, Journal Year: 2023, Volume and Issue: 87(3)

Published: June 26, 2023

Type III secretion systems (T3SSs) are utilized by Gram-negative pathogens to enhance their pathogenesis. This system is associated with the delivery of effectors through a needle-like structure from bacterial cytosol directly into target eukaryotic cell.

Language: Английский

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Till evolution do us part: The diversity of symbiotic associations across populations of Philaenus spittlebugs

Environmental Microbiology, Journal Year: 2023, Volume and Issue: 25(11), P. 2431 - 2446

Published: Aug. 1, 2023

Symbiotic bacteria have played crucial roles in the evolution of sap-feeding insects and can strongly affect host function. However, their diversity distribution within species are not well understood; we do know to what extent environmental factors or associations with other may microbial community profiles. We addressed this question Philaenus spittlebugs by surveying both insect bacterial marker gene amplicons across multiple populations. Host mitochondrial sequence data confirmed morphology-based identification six revealed two divergent clades spumarius. All them hosted primary symbiont Sulcia that was almost always accompanied Sodalis. Interestingly, populations individuals often differed presence Sodalis variants, suggestive intra-genome 16S rRNA variant polymorphism combined rapid genome and/or recent additional infections replacements co-primary symbiont. The prevalence facultative endosymbionts, including Wolbachia, Rickettsia, Spiroplasma, varied among Notably, cytochrome I oxidase (COI) amplicon also showed nearly a quarter P. spumarius were infected parasitoid flies (Verralia aucta). One Wolbachia operational taxonomic units (OTUs) exclusively present Verralia-parasitized specimens, parasitoids as source highlighting utility sequencing microbiome studies.

Language: Английский

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Prevalence of Heritable Symbionts in Parisian Bedbugs (Hemiptera: Cimicidae)

Environmental Microbiology Reports, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 29, 2025

ABSTRACT Like many insects, the biology of bedbugs is impacted by a range partner heritable microbes. Three maternally inherited symbionts are recognised: Wolbachia (an obligate partner), Symbiopectobacterium purcellii strain Sy Clec, and Candidatus Tisiphia sp. (facultative typically present in some but not all individuals). Past work had examined presence these microbes from established laboratory lines, broader field samples. We therefore deployed targeted endpoint PCR assays to determine symbiont infection status for 50 collected 10 districts Paris during 2023 outbreak. All three were found be broadly across Cimex lectularius samples, with ‐ Tisiphia‐ triple most commonly observed. A minority individuals lacked either one or both facultative symbionts. Five mtDNA haplotypes observed COI barcode region, infections haplotypes, indicating that recent invasion event. conclude Parisian bedbug outbreak was which host's secondary at high‐frequency coinfections, an important uncharacterised component populations.

Language: Английский

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From insect endosymbiont to phloem colonizer: comparative genomics unveils the lifestyle transition of phytopathogenic Arsenophonus strains

mSystems, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

ABSTRACT Bacteria infecting the plant phloem represent a growing threat worldwide. While these organisms often resist in vitro culture, they multiply both sieve elements and hemipteran vectors. Such cross-kingdom parasitic lifestyle has emerged diverse taxa via distinct ecological routes. In genus Arsenophonus , pathogens “ Candidatus phytopathogenicus” (Ap) Ca . Phlomobacter fragariae” (Pf) have evolved from insect endosymbionts, but genetic mechanisms underlying this transition not been explored. To fill gap, we obtained genomes of strains host metagenomes. The resulting assemblies are highly similar size functional repertoire, rich viral sequences, closely resemble several facultative endosymbiotic sap-sucking hemipterans. However, phylogenomic analysis demonstrated origins, as Ap belongs to Triatominarum ” clade, whereas Pf represents distant species. We identified set orthologs encoded only by genus, including hydrolytic enzymes likely targeting substrates. particular, bacteria encode putative cell wall-degrading cysteine peptidases related xylellain, papain-like peptidase Xylella fastidiosa for which close homologs found Pseudomonadota vasculature. silico predictions gene expression analyses further support role during colonization shared orthologs. conclude that double emergence phytopathogenicity may mediated few horizontal transfer events, involving genes acquired other phytopathogens. IMPORTANCE investigate bacterial lifestyle. focus on two belonging : fragariae.” Both cause economically significant pathologies, among endosymbionts. Our genomic show associated with hemipterans, suggesting recent shift. Importantly, although phytopathogenic belong clades, share small unique pangenome. provide evidence produce secreted target acquisition exchange thus played pivotal strains.

Language: Английский

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Description of a new genus of the Pectobacteriaceae family isolated from water in coastal brackish wetlands of the French Camargue region, Prodigiosinella gen. nov., including the new species Prodigiosinella aquatilis sp. nov

Systematic and Applied Microbiology, Journal Year: 2024, Volume and Issue: 47(2-3), P. 126497 - 126497

Published: Feb. 21, 2024

The Pectobacteriaceae family comprises plant pathogens able to provoke diverse diseases, including maceration due the production of pectinases disrupting cell wall. To better understand their diversity, a survey pectinolytic bacteria was performed in brackish lakes French region La Camargue near Mediterranean Sea. genome six atypical isolates sequenced; size is around 4.8 5.0 Mb, plasmid 59 61 kb; G+C values range from 49.1 49.3 mol%. Phylogenetic analyses indicated that novel strains form new clade branches at basis group encompassing genera Lonsdalea, Musicola, and Dickeya. Based on phenotypic, genomic phylogenetic characteristics, we propose creation genus with name Prodigiosinella gen. nov. Both phenotypic separated into two distinct subgroups, G1 G2. type strain LS101

Language: Английский

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16S rRNA Gene Sequencing of Six Psyllid Species of the Family Carsidaridae Identified Various Bacteria Including <i>Symbiopectobacterium</i>

Microbes and Environments, Journal Year: 2023, Volume and Issue: 38(3), P. n/a - n/a

Published: Jan. 1, 2023

Psyllids (Hemiptera: Sternorrhyncha: Psylloidea) are plant sap-sucking insects that closely associated with various microbes. To obtain a more detailed understanding of the ecological and evolutionary behaviors microbes in Psylloidea, bacterial populations six psyllid species, belonging to family Carsidaridae, were analyzed using high-throughput amplicon sequencing 16S rRNA gene. The majority secondary symbionts identified present study gammaproteobacteria, particularly those order Enterobacterales, including Arsenophonus Sodalis, which lineages found wide variety insect hosts. Additionally, Symbiopectobacterium, another Enterobacterales lineage, has recently been recognized increasingly shown be vertically transmitted mutualistic invertebrates, was for first time Psylloidea. This lineage is related Pectobacterium spp., pathogens, but forms distinct clade exhibiting no pathogenicity plants. Non-Enterobacterales gammaproteobacteria Acinetobacter, Pseudomonas (both Pseudomonadales), Delftia, Comamonas Burkholderiales), Xanthomonas (Xanthomonadales), putative pathogen. Regarding alphaproteobacteria, three Wolbachia (Rickettsiales) supergroup B, major group lineages, detected four species. In addition, O, minor one These results suggest pervasive transfer among animals plants, providing deeper insights into evolution interactions these organisms.

Language: Английский

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Bacterial Endosymbionts Identified From Leafhopper (Hemiptera: Cicadellidae) Vectors of Phytoplasmas

Environmental Entomology, Journal Year: 2023, Volume and Issue: 52(2), P. 243 - 253

Published: March 4, 2023

Abstract Insects often harbor bacterial endosymbionts that provide them with nutritional benefit or protection against natural enemies, plant defenses, insecticides, and abiotic stresses. Certain may also alter acquisition transmission of pathogens by insect vectors. We identified from four leafhopper vectors (Hemiptera: Cicadellidae) ‘Candidatus Phytoplasma’ species direct sequencing 16S rDNA confirmed endosymbiont presence identity species-specific conventional PCR. examined three Ca. Phytoplasma pruni, causal agent cherry X-disease [Colladonus geminatus (Van Duzee), Colladonus montanus reductus Euscelidius variegatus (Kirschbaum)] – a vector trifolii, the potato purple top disease [Circulifer tenellus (Baker)]. Direct two obligate leafhoppers, ‘Ca. Sulcia’ Nasuia’, which are known to produce essential amino acids lacking in leafhoppers’ phloem sap diet. About 57% C. harbored endosymbiotic Rickettsia. Yamatotoia cicadellidicola’ variegatus, providing just second host record for this endosymbiont. Circulifer facultative Wolbachia, although average infection rate was only 13% all males were Wolbachia-uninfected. A significantly greater percentage Wolbachia-infected Ci. adults than uninfected carried P. suggesting Wolbachia increase insect’s ability tolerate acquire pathogen. Results our study foundation continued work on interactions between endosymbionts, phytoplasma.

Language: Английский

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Development, feeding, and sex shape the relative quantity of the nutritional obligatory symbiont Wolbachia in bed bugs

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 7, 2024

The common bed bug,

Language: Английский

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Two species with a peculiar evolution within the genus Pectobacterium suggest adaptation to a new environmental niche

Environmental Microbiology, Journal Year: 2023, Volume and Issue: 25(11), P. 2465 - 2480

Published: Aug. 7, 2023

Abstract Historically, research on Soft Rot Pectobacteriacea (SRP) has focused economically important crops and ornamentals knowledge of these bacteria outside the plant context remains poorly investigated. Recently, two closely related species Pectobacterium aquaticum quasiaquaticum were isolated from water have not been any yet. To identify distinctive characteristics species, we performed a comparative genomic analysis 80 genomes representing 19 an evolutionary reconstruction. Both underwent reduction in genome size associated with high pseudogene content. A gene loss was predicted at emergence both species. Among 199 families missing P. but present least 80% other genomes, COG identified many genes involved nutrient transport systems. In addition, type II secreted proteins also Phenotypic revealed that had reduced pectinolytic activity, biofilm formation defect, highly motile virulence several plants. These phenotypic data suggest ecological niche may differ

Language: Английский

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Obligate Gut Symbiotic Association with Caballeronia in the Mulberry Seed Bug Paradieuches dissimilis (Lygaeoidea: Rhyparochromidae)

Microbial Ecology, Journal Year: 2022, Volume and Issue: 86(2), P. 1307 - 1318

Published: Sept. 30, 2022

Language: Английский

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