Unveiling triclosan biodegradation: Novel metabolic pathways, genomic insights, and global environmental adaptability of Pseudomonas sp. strain W03
Lan Qiu,
No information about this author
Xiaoyuan Guo,
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Hojae Shim
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et al.
Journal of Hazardous Materials,
Journal Year:
2025,
Volume and Issue:
488, P. 137313 - 137313
Published: Jan. 21, 2025
Language: Английский
Triclosan Dioxygenase: A Novel Two-component Rieske Nonheme Iron Ring-hydroxylating Dioxygenase Initiates Triclosan Degradation
Yiran Yin,
No information about this author
Hao Ren,
No information about this author
Hao Wu
No information about this author
et al.
Environmental Science & Technology,
Journal Year:
2024,
Volume and Issue:
58(31), P. 13833 - 13844
Published: July 16, 2024
The
emerging
contaminant
triclosan
(TCS)
is
widely
distributed
both
in
surface
water
and
wastewater
poses
a
threat
to
aquatic
organisms
human
health
due
its
resistance
degradation.
dioxygenase
enzyme
TcsAB
has
been
speculated
perform
the
initial
degradation
of
TCS,
but
precise
catalytic
mechanism
remains
unclear.
In
this
study,
function
was
elucidated
using
multiple
biochemical
molecular
biology
methods.
Escherichia
coli
BL21(DE3)
heterologously
expressing
tcsAB
from
Sphingomonas
sp.
RD1
converted
TCS
2,4-dichlorophenol.
belongs
group
IA
family
two-component
Rieske
nonheme
iron
ring-hydroxylating
dioxygenases.
highest
amino
acid
identity
TcsA
large
subunits
other
dioxygenases
same
only
35.50%,
indicating
that
novel
dioxygenase.
Mutagenesis
residues
near
substrate
binding
pocket
decreased
TCS-degrading
activity
narrowed
spectrum,
except
for
TcsAF343A
mutant.
A
meta-analysis
1492
samples
treatment
systems
worldwide
revealed
tcsA
genes
are
distributed.
This
study
first
report
TCS-specific
responsible
TCS.
Studying
microbial
crucial
removing
pollutant
environment.
Language: Английский
Comamonas halotolerans sp. nov., isolated from the faecal sample of a zoo animal, Naemorhedus caudatus
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,
Journal Year:
2025,
Volume and Issue:
75(1)
Published: Jan. 29, 2025
Strain
NoAH
T
(=KACC
23135
=JCM
35999
),
a
novel
Gram-negative,
motile
bacterium
with
rod-shaped
morphology,
was
isolated
from
the
zoo
animal
faecal
samples,
specifically
long-tailed
goral
species
Naemorhedus
caudatus
.
The
bacterial
strain
grew
optimally
in
nutrient
broth
medium
under
following
conditions:
1–2%
(w/v)
NaCl,
pH
7–8
and
30
°C.
exhibited
high
tolerance
to
ability
tolerate
up
7%
NaCl.
Based
on
phylogenetic
analyses
using
16S
rRNA
gene
sequencing,
found
have
closest
relatedness
Comamonas
jiangduensis
YW1
(98.5%),
aquatica
ATCC
11330
(97.9%),
resistens
KCTC
82561
fluminis
CJ34
(97.7%)
suwonensis
EJ-4
(97.6%).
genome
size
genomic
DNA
G+C
content
of
were
4.05
Mbp
55.9
mol%,
respectively.
A
whole-genome-level
comparison
C.
YW
,
kerstersii
LMG
3475
NBRC
14918
terrigena
12685
revealed
orthologous
average
nucleotide
identity
values:
80.1,
79.0,
78.6,
76.3
75.2%,
major
polar
lipids
phosphatidylethanolamine,
phosphatidylglycerol
diphosphatidylglycerol.
Considering
our
findings
chemotaxonomic,
genotypic
phenotypic
characteristics,
is
identified
as
within
genus
for
which
name
halotolerans
sp.
nov.
proposed.
Language: Английский
Computer-directed rational engineering of dioxygenase TcsAB for triclosan biodegradation under cold conditions
Yiran Yin,
No information about this author
Xinfeng Yu,
No information about this author
Zongxin Tao
No information about this author
et al.
Applied and Environmental Microbiology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 5, 2025
The
dioxygenase
TcsAB
is
a
specific
involved
in
the
initial
biodegradation
of
broad-spectrum
antibacterial
agent
triclosan
(TCS).
However,
it
exhibits
significantly
reduced
activity
under
cold
conditions.
In
this
study,
computer-directed
approach
combining
loop
engineering
and
N-terminal
truncation
was
utilized
to
decrease
thermostability
TcsAB,
thereby
enhancing
its
catalytic
environments.
iterative
mutant
(TcsAY277P/F279P/S311W/A313WTcsBN-terminal
truncation)
exhibited
2.54-fold
greater
efficiency
than
wild
type
at
15°C.
Molecular
dynamics
simulations
showed
that
mutations
introduced
substrate-binding
pocket
increased
flexibility,
leading
enhanced
through
binding
more
advantageous
conformation.
This
modified
employed
as
biological
component,
Pseudomonas
knackmussii
B13
used
chassis
cell
construct
an
engineered
strain
for
efficient
degradation
TCS
low
temperatures.
objective
enhance
capacity
bioremediation
natural
Insights
gained
from
study
may
inform
rational
redesign
enzymes
related
robustness
emerging
contaminants.IMPORTANCEThe
presence
surface
water
wastewater
poses
significant
risk
aquatic
organisms
human
health
due
high
resistance
degradation.
pollution
environment
metabolic
processes
microorganisms
represents
effective
remediation
strategy.
only
enzyme
has
been
identified
responsible
TCS.
Nevertheless,
markedly
diminished
actual
ambient
temperature
frequently
lower
optimum
reaction,
maintaining
30°C
reaction
condition
results
costs
energy
consumption
removal.
Accordingly,
low-temperature
will
facilitate
realistic
removal
aqueous
environment.
Language: Английский