Computer-directed rational engineering of dioxygenase TcsAB for triclosan biodegradation under cold conditions DOI Creative Commons
Yiran Yin, Xinfeng Yu,

Zongxin Tao

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

The dioxygenase TcsAB is a specific involved in the initial biodegradation of broad-spectrum antibacterial agent triclosan (TCS). However, it exhibits significantly reduced activity under cold conditions. In this study, computer-directed approach combining loop engineering and N-terminal truncation was utilized to decrease thermostability TcsAB, thereby enhancing its catalytic environments. iterative mutant (TcsAY277P/F279P/S311W/A313WTcsBN-terminal truncation) exhibited 2.54-fold greater efficiency than wild type at 15°C. Molecular dynamics simulations showed that mutations introduced substrate-binding pocket increased flexibility, leading enhanced through binding more advantageous conformation. This modified employed as biological component, Pseudomonas knackmussii B13 used chassis cell construct an engineered strain for efficient degradation TCS low temperatures. objective enhance capacity bioremediation natural Insights gained from study may inform rational redesign enzymes related robustness emerging contaminants.IMPORTANCEThe presence surface water wastewater poses significant risk aquatic organisms human health due high resistance degradation. pollution environment metabolic processes microorganisms represents effective remediation strategy. only enzyme has been identified responsible TCS. Nevertheless, markedly diminished actual ambient temperature frequently lower optimum reaction, maintaining 30°C reaction condition results costs energy consumption removal. Accordingly, low-temperature will facilitate realistic removal aqueous environment.

Language: Английский

Unveiling triclosan biodegradation: Novel metabolic pathways, genomic insights, and global environmental adaptability of Pseudomonas sp. strain W03 DOI
Lan Qiu,

Xiaoyuan Guo,

Hojae Shim

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 488, P. 137313 - 137313

Published: Jan. 21, 2025

Language: Английский

Citations

1
Triclosan Dioxygenase: A Novel Two-component Rieske Nonheme Iron Ring-hydroxylating Dioxygenase Initiates Triclosan Degradation DOI
Yiran Yin,

Hao Ren,

Hao Wu

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(31), P. 13833 - 13844

Published: July 16, 2024

The emerging contaminant triclosan (TCS) is widely distributed both in surface water and wastewater poses a threat to aquatic organisms human health due its resistance degradation. dioxygenase enzyme TcsAB has been speculated perform the initial degradation of TCS, but precise catalytic mechanism remains unclear. In this study, function was elucidated using multiple biochemical molecular biology methods. Escherichia coli BL21(DE3) heterologously expressing tcsAB from Sphingomonas sp. RD1 converted TCS 2,4-dichlorophenol. belongs group IA family two-component Rieske nonheme iron ring-hydroxylating dioxygenases. highest amino acid identity TcsA large subunits other dioxygenases same only 35.50%, indicating that novel dioxygenase. Mutagenesis residues near substrate binding pocket decreased TCS-degrading activity narrowed spectrum, except for TcsAF343A mutant. A meta-analysis 1492 samples treatment systems worldwide revealed tcsA genes are distributed. This study first report TCS-specific responsible TCS. Studying microbial crucial removing pollutant environment.

Language: Английский

Citations

8
Comamonas halotolerans sp. nov., isolated from the faecal sample of a zoo animal, Naemorhedus caudatus DOI
Yerim Park, Bitnara Kim, Jihyeon Min

et al.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2025, Volume and Issue: 75(1)

Published: Jan. 29, 2025

Strain NoAH T (=KACC 23135 =JCM 35999 ), a novel Gram-negative, motile bacterium with rod-shaped morphology, was isolated from the zoo animal faecal samples, specifically long-tailed goral species Naemorhedus caudatus . The bacterial strain grew optimally in nutrient broth medium under following conditions: 1–2% (w/v) NaCl, pH 7–8 and 30 °C. exhibited high tolerance to ability tolerate up 7% NaCl. Based on phylogenetic analyses using 16S rRNA gene sequencing, found have closest relatedness Comamonas jiangduensis YW1 (98.5%), aquatica ATCC 11330 (97.9%), resistens KCTC 82561 fluminis CJ34 (97.7%) suwonensis EJ-4 (97.6%). genome size genomic DNA G+C content of were 4.05 Mbp 55.9 mol%, respectively. A whole-genome-level comparison C. YW , kerstersii LMG 3475 NBRC 14918 terrigena 12685 revealed orthologous average nucleotide identity values: 80.1, 79.0, 78.6, 76.3 75.2%, major polar lipids phosphatidylethanolamine, phosphatidylglycerol diphosphatidylglycerol. Considering our findings chemotaxonomic, genotypic phenotypic characteristics, is identified as within genus for which name halotolerans sp. nov. proposed.

Language: Английский

Citations

0
Computer-directed rational engineering of dioxygenase TcsAB for triclosan biodegradation under cold conditions DOI Creative Commons
Yiran Yin, Xinfeng Yu,

Zongxin Tao

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

The dioxygenase TcsAB is a specific involved in the initial biodegradation of broad-spectrum antibacterial agent triclosan (TCS). However, it exhibits significantly reduced activity under cold conditions. In this study, computer-directed approach combining loop engineering and N-terminal truncation was utilized to decrease thermostability TcsAB, thereby enhancing its catalytic environments. iterative mutant (TcsAY277P/F279P/S311W/A313WTcsBN-terminal truncation) exhibited 2.54-fold greater efficiency than wild type at 15°C. Molecular dynamics simulations showed that mutations introduced substrate-binding pocket increased flexibility, leading enhanced through binding more advantageous conformation. This modified employed as biological component, Pseudomonas knackmussii B13 used chassis cell construct an engineered strain for efficient degradation TCS low temperatures. objective enhance capacity bioremediation natural Insights gained from study may inform rational redesign enzymes related robustness emerging contaminants.IMPORTANCEThe presence surface water wastewater poses significant risk aquatic organisms human health due high resistance degradation. pollution environment metabolic processes microorganisms represents effective remediation strategy. only enzyme has been identified responsible TCS. Nevertheless, markedly diminished actual ambient temperature frequently lower optimum reaction, maintaining 30°C reaction condition results costs energy consumption removal. Accordingly, low-temperature will facilitate realistic removal aqueous environment.

Language: Английский

Citations

0