A Klebsiella pneumoniae ST307 outbreak clone from Germany demonstrates features of extensive drug resistance, hypermucoviscosity, and enhanced iron acquisition

Genome Medicine, Journal Year: 2020, Volume and Issue: 12(1)

Published: Dec. 1, 2020

Abstract Background Antibiotic-resistant Klebsiella pneumoniae are a major cause of hospital- and community-acquired infections, including sepsis, liver abscess, pneumonia, driven mainly by the emergence successful high-risk clonal lineages. The K. sequence type (ST) 307 lineage has appeared in several different parts world after first being described Europe 2008. From June to October 2019, we recorded an outbreak extensively drug-resistant ST307 four medical facilities north-eastern Germany. Methods Here, investigated these isolates those from subsequent cases same facilities. We performed whole-genome sequencing study phylogenetics, microevolution, plasmid transmission, as well phenotypic experiments growth curves, hypermucoviscosity, siderophore secretion, biofilm formation, desiccation resilience, serum survival, heavy metal resistance for in-depth characterization this clone. Results Phylogenetics suggest homogenous phylogram with sub-clades containing either only one patient or originating patients, suggesting inter-patient transmission. identified three large plasmids, carrying NDM-1, CTX-M-15, OXA-48, which likely donated other STs even bacterial species (e.g., Enterobacter cloacae ) within clinical settings. Several chromosomally plasmid-encoded, hypervirulence-associated virulence factors yersiniabactin, metabolite transporter, aerobactin, genes) were addition. While growth, comparable control strains, results secretion hypermucoviscosity revealed superiority clone, similar archetypical, hypervirulent strain (hvKP1). Conclusions combination extensive drug virulence, partly conferred through “mosaic” both antibiotic features, demonstrates serious public health implications.

Language: Английский

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The intersection of capsule gene expression, hypermucoviscosity and hypervirulence in Klebsiella pneumoniae

Current Opinion in Microbiology, Journal Year: 2020, Volume and Issue: 54, P. 95 - 102

Published: Feb. 12, 2020

Language: Английский

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Antibiotic Resistance and Virulence Profiles of Klebsiella pneumoniae Strains Isolated From Different Clinical Sources

Frontiers in Cellular and Infection Microbiology, Journal Year: 2021, Volume and Issue: 11

Published: Sept. 1, 2021

Klebsiella pneumoniae is a Gram-negative bacterium capable of colonizing, invading, and causing infections in different anatomical sites the human body. Its ability to evade immune system, its increasing antimicrobial resistance emergence hypervirulent pathotypes have become major challenge medical field. In this study, 127 strains from clinical sources (urine, respiratory tract or blood) were characterized for resistance, presence virulence factor genes, serum hypermucoviscosity form biofilms. Specific characteristics uropathogenic examined compared with other groups. Differences found between urine groups strains. Urine showed highest antibiotic (64.91%) blood (63.64%) (51.35%) as well extended-spectrum beta-lactamases (ESBL) production. These also statistically significant high fosfomycin (24.56%) (p = 0.008). Regarding virulence, 84.21% presented uge gene, showing difference 0.03) sources, indicating possible role gene development urinary infection. addition, 46% biofilm-forming belonged sample group 0.043). conclusion, K. isolated samples higher ESBL production, those samples. The rapid spread these concern, new therapeutic alternatives are essential mitigate their harmful effects.

Language: Английский

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The Small Protein RmpD Drives Hypermucoviscosity in Klebsiella pneumoniae

mBio, Journal Year: 2020, Volume and Issue: 11(5)

Published: Sept. 21, 2020

has a remarkable ability to cause wide range of human diseases. It is divided into two broad classes: classical strains that are notable problem in health care settings due multidrug resistance, and hypervirulent (hv) historically drug sensitive but able establish disease immunocompetent hosts. Alarmingly, there been an increased frequency clinical isolates have both resistance hv-associated genes. One such gene,

Language: Английский

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Evolution of Outbreak-Causing Carbapenem-Resistant Klebsiella pneumoniae ST258 at a Tertiary Care Hospital over 8 Years

mBio, Journal Year: 2019, Volume and Issue: 10(5)

Published: Sept. 4, 2019

The carbapenem class of antibiotics is invaluable for the treatment selected multidrug-resistant Gram-negative pathogens. continued transmission carbapenem-resistant bacteria such as ST258 K. pneumoniae serious global public health concern, options these infections are limited. This genomic epidemiologic investigation traced natural history in a single care setting over nearly decade. We found that distinct subpopulations have caused both device-associated and ward-associated outbreaks, some populations remain endemic within our hospital to present day. finding virulence determinants among emergent clones supports idea convergent evolution drug-resistant virulent CRKP strains highlights need surveillance, prevention, control efforts address evolving setting.

Language: Английский

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