Construction and Validation of a Predictive Model for Mortality Risk in Patients with Acinetobacter baumannii Bloodstream Infection DOI Creative Commons
X H Li,

Donghao Cai,

Chuangchuang Mei

et al.

Infection and Drug Resistance, Journal Year: 2024, Volume and Issue: Volume 17, P. 5247 - 5260

Published: Nov. 1, 2024

To develop and validate a predictive model for the risk of death in patients with

Language: Английский

Pathogenicity and virulence of Acinetobacter baumannii : Factors contributing to the fitness in healthcare settings and the infected host DOI Creative Commons
Massimiliano Lucidi, Daniela Visaggio, Antonella Migliaccio

et al.

Virulence, Journal Year: 2023, Volume and Issue: 15(1)

Published: Dec. 6, 2023

Acinetobacter baumannii is a common cause of healthcare-associated infections and hospital outbreaks, particularly in intensive care units. Much the success A. relies on its genomic plasticity, which allows rapid adaptation to adversity stress. The capacity acquire novel antibiotic resistance determinants tolerance stresses encountered environment promote spread among patients long-term contamination healthcare setting. This review explores virulence factors physiological traits contributing infection environment. Several cell-associated secreted involved biofilm formation, cell adhesion, invasion, persistence host, as well xeric stress imposed by settings, are illustrated give reasons for pathogen.

Language: Английский

Citations

37
The Structural Basis of Substrate Selectivity of the Acinetobactin Biosynthetic Adenylation Domain, BasE DOI Creative Commons
Syed Fardin Ahmed, Andrew M. Gulick

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108413 - 108413

Published: March 1, 2025

Language: Английский

Citations

1
Genomics of Acinetobacter baumannii iron uptake DOI Creative Commons
Irene Artuso,

Harsh Poddar,

Benjamin A. Evans

et al.

Microbial Genomics, Journal Year: 2023, Volume and Issue: 9(8)

Published: Aug. 7, 2023

Iron is essential for growth in most bacteria due to its redox activity and role metabolic reactions; it a cofactor many bacterial enzymes. The bacterium Acinetobacter baumannii multidrug-resistant nosocomial pathogen. A. responds low iron availability imposed by the host through exploitation of multiple iron-acquisition strategies, which are likely deliver cell under variety environmental conditions, including human animal infection. To date, six different gene clusters active uptake have been described , encoding protein systems involved (i) ferrous ( feo ); (ii) haem hemT hemO (iii) synthesis transport baumannoferrin(s) bfn ), acinetobactin bas / bau ) fimsbactin(s) fbs siderophores. Here we describe structure, distribution phylogeny iron-uptake among >1000 genotypically diverse isolates, showing that very prevalent across dataset, whereas additional haem-uptake system only present portion dataset cluster rare. Since expression can be linked virulence, presence may contributed success some clones.

Language: Английский

Citations

16
Expanding the Substrate Selectivity of the Fimsbactin Biosynthetic Adenylation Domain, FbsH DOI
Syed Fardin Ahmed, Adam Balutowski, Jinping Yang

et al.

ACS Chemical Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Nonribosomal peptide synthetases (NRPSs) produce diverse natural products including siderophores, chelating agents that many pathogenic bacteria to survive in low iron conditions. Engineering NRPSs siderophore analogs could lead the generation of novel antibiotics and imaging take advantage this unique uptake system bacteria. The highly antibiotic-resistant

Language: Английский

Citations

5
Bioactivity of Rhizospheric Acinetobacter baumannii Siderophore Combined with Antibiotics Against Lower Respiratory Tract Pathogenic Bacteria DOI Creative Commons

Rafal Moayad Abdul-Latif,

Amel Hussaein Mussa

Nature Environment and Pollution Technology, Journal Year: 2025, Volume and Issue: 24(S1), P. 285 - 291

Published: Jan. 16, 2025

The study focused on extracting and purifying siderophore produced by Acinetobacter baumannii isolated from rhizospheric soil in Baghdad city evaluating its bioactivity both independently combination with selected antibiotics. Bacterial identification was performed using CHROM agar, biochemical, physiological tests, confirmation via PCR amplification of the 16S rDNA housekeeping gene. extracted ethyl acetate after culturing bacteria succinate broth purified through HPLC, detected at a wavelength 403 nm. A total 38 bacterial isolates were obtained lower respiratory tract infections, including Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, baumannii, Staphylococcus aureus, Serratia marcescens. Antibiotic susceptibility testing 13 antibiotics showed highest resistance rates to ampicillin (65.7%) ceftriaxone (63.1%), while lowest observed amikacin (15.7%). synergistic activity combined sub-MIC concentrations ceftriaxone, ceftazidime, gentamycin tested against multidrug-resistant (MDR) isolates. most significant antibacterial S. whereas minimal effect noted A. baumannii. In conclusion, successfully identified infections. exhibited notable aureus but ineffective

Language: Английский

Citations

0
Exploring the clinical outcomes and molecular characteristics of Acinetobacter baumannii bloodstream infections: a study of sequence types, capsular types, and drug resistance in China DOI Creative Commons

Jiao Chen,

Yanting Shao, Zhibin Cheng

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 17, 2025

Bloodstream infections (BSIs) caused by Acinetobacter baumannii have been associated with high mortality. To improve the outcomes of patients, this study explored clinical characteristics and patients BSIs, as well phenotypic genomic these isolates. A retrospective cohort was conducted involving A. BSIs cases from 2020 to 2023 in a tertiary hospital. The all isolates were evaluated. Virulence phenotypes evaluated using growth curve, biofilm-forming assay, antiserum complement killing, G.mellonella killing assay. Furthermore, whole-genome sequencing (WGS) utilized analyze characteristics. 30-day mortality rate 67 55.22%. Patients death group had significantly lower platelet counts higher CRP levels than those survival group. Additionally, rates antibiotic use (≥2 classes) greater carbapenem exposure observed. Among isolates, CRAb accounted for 80.6%, ST2 76.12%, KL2/3/7/77/160 65.67%. predominant KL type KL3, found 19.4% All CRAb. 90.7% coharbored blaOXA-23 blaOXA-66 , while one blaNDM-1 . Compared non-ST2 non infections, (66.0% vs. 23.5%, P=0.002; 65.90% 34.78%, P=0.015). underwent more invasive procedures, received two or antibiotics therapy before isolation, serum albumin levels. These exhibited resistance antimicrobial agents. No significant differences virulence observed between groups, except biofilm formation groups (P=0.002). However, harbored genes related iron uptake formation. is high. It great significance clinicians pay attention risk factors identify ST types strains causing infection at an early stage.

Language: Английский

Citations

0
Positron Emission Tomography Imaging of Acinetobacter baumannii Infection: Comparison of Gallium-68 Labeled Siderophores DOI Creative Commons
Kateřina Bendová, Kristýna Krasulová, Barbora Neužilová

et al.

ACS Infectious Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Acinetobacter baumannii (AB) is an opportunistic pathogen with growing clinical relevance due to its increasing level of antimicrobial resistance in the last few decades. In event AB hospital outbreak, fast detection and localization crucial, prevent further spread. However, contemporary diagnostic tools do not always meet requirements for rapid accurate diagnosis. For this reason, we report here possibility using gallium-68 labeled siderophores, bacterial iron chelators, positron emission tomography imaging infections. our study, radiolabeled several siderophores tested their vitro uptake cultures. Based on results properties studied selected two them vivo testing infectious models. Both ferrioxamine E ferrirubin, showed promising characteristics. vivo, observed pharmacokinetics no excessive accumulation organs other than excretory normal mice. We demonstrated that accumulate AB-infected tissue three animal models: a murine model myositis, dorsal wound infection rat pneumonia. These suggest both Ga-68 could be used PET infection.

Language: Английский

Citations

0
Emergence and global spread of a dominant multidrug-resistant clade within Acinetobacter baumannii DOI Creative Commons

Shengkai Li,

Guilai Jiang, Shengke Wang

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 21, 2025

The proliferation of multi-drug resistant (MDR) bacteria is driven by the global spread epidemic lineages that accumulate antimicrobial resistance genes (ARGs). Acinetobacter baumannii, a leading cause nosocomial infections, displays to most frontline antimicrobials and represents significant challenge public health. In this study, we conduct comprehensive genomic analysis over 15,000 A. baumannii genomes identify predominant super-lineage (ESL) accounting for approximately 70% isolates. Through hierarchical classification ESL into distinct lineages, clusters, clades, identified stepwise evolutionary trajectory responsible worldwide expansion transmission last eight decades. We observed rise previously unrecognized Clade 2.5.6, which emerged in East Asia 2006. clade linked ongoing acquisition ARGs virulence factors facilitated genetic recombination. Our results highlight necessity One Health-oriented research interventions address MDR pathogen. authors isolates, revealing dominant multidrug-resistant (accounting isolates), with 2.5.6 emerging

Language: Английский

Citations

0
Gallium nitrate inhibits multidrug-resistant Acinetobacter baumannii isolated from bloodstream infection by disrupting multiple iron-dependent metabolic processes DOI Creative Commons
Zhuocheng Yao, Kaihang Yu, Changrui Qian

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 14, 2025

Language: Английский

Citations

0
Transcriptomic Insights into the Virulence of Acinetobacter baumannii During Infection: Role of Iron Uptake and Siderophore Production Genes DOI
Kah Ern Ten, Sadequr Rahman, Hock Siew Tan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 15, 2024

Abstract Acinetobacter baumannii is a top-priority pathogen as classified by the World Health Organisation. It causes life-threatening infections in immunocompromised patients, resulting prolonged hospitalisation and high mortality. Increasing cases of community-acquired A. with rapid progression severe have been reported. This study used previously described Galleria mellonella infection model to investigate virulence mechanisms community strain C98 (Ab-C98) via transcriptomic analysis using direct RNA sequencing. showed greater killing more colonisation larvae than clinical reference (ATCC BAA1605). Differential gene expression revealed significant upregulation three major iron clusters: acinetobactin baumannoferrin clusters for siderophore production Feo system ferrous uptake. Targeted knockout genes ( basC , bfnD isochorismatase family protein) significantly attenuated mutants minimal impact on bacterial growth vivo . Overall, this highlights protein pathogenicity As these targets are highly conserved closely related pittii lactucae they could serve potential therapeutic developing new antivirulence agents combat pathogens.

Language: Английский

Citations

1