Conserved patterns of sequence diversification provide insight into the evolution of two-component systems in Enterobacteriaceae

Microbial Genomics, Journal Year: 2024, Volume and Issue: 10(3)

Published: March 19, 2024

Two-component regulatory systems (TCSs) are a major mechanism used by bacteria to sense and respond their environments. Many of the same TCSs biologically diverse organisms with different needs, suggesting that functions TCS must evolve. To explore this topic, we analysed amino acid sequence divergence patterns large set broadly conserved across branches Enterobacteriaceae , family Gram-negative includes biomedically important genera such as Salmonella Escherichia Klebsiella others. Our analysis revealed trends in how sequences change proteins or functional domains TCS, lineages. Based on these trends, identified individual exhibit atypical evolutionary patterns. We observed relative extent which given varies lineages is generally well conserved, unveiling hierarchy conservation EnvZ/OmpR most TCS. provide evidence that, for divergent analysed, PmrA/PmrB, alleles were horizontally acquired family, PmrA/PmrB variants have highly signal-sensing domains. Collectively, study sheds light evolve, serves compendium diverged over course evolution.

Language: Английский

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Interplay between porin deficiency, fitness, and virulence in carbapenem-non-susceptible Pseudomonas aeruginosa and Enterobacteriaceae

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(2), P. e1012902 - e1012902

Published: Feb. 7, 2025

The increasing resistance of Gram-negative bacteria to last resort antibiotics, such as carbapenems, is particularly concern it a significant cause global health threat. In this context, there an urgent need for better understanding underlying mechanisms leading antimicrobial in order limit its diffusion and develop new therapeutic strategies. review, we focus on the specific role porins carbapenem-resistance Enterobacteriaceae Pseudomonas aeruginosa , which are major human pathogens. Porins outer membrane proteins, play key bacterial permeability allow nutrients enter toxic waste leave. However, these channels also “Achilles’ heel” antibiotics can pass through them reach their target kill bacteria. After describing normal structures pathways regulating expression porins, discuss strategies implemented by access carbapenems cytoplasmic target. We further examine real impact changes susceptibility. Finally, decipher what effect fitness virulence. Our goal integrate all findings give overview how modify face antibiotic selective pressure trying not induce cost.

Language: Английский

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Multifaceted Evolution of the PhoPQ Two‐Component System in Salmonella enterica Enhanced the Expression of Horizontally Acquired Virulence Genes

Molecular Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

For a bacterium to adapt new environmental niche, its regulatory networks must evolve effectively sense and respond cues within that niche. bacterial pathogens, which encounter harsh dynamic host niches require efficient coordination between detecting regulating virulence genes, this process is key aspect of how properties evolve. Here, we investigate widely conserved two-component system (TCS), PhoP/PhoQ (PhoPQ), evolved in Salmonella enterica adopt role as master regulator gene expression species-specific intracellular niche: the Salmonella-containing vacuole (SCV). By comparing PhoPQ with closely related Escherichia coli ortholog, demonstrate optimizing required multifaceted evolution several functional domains establish distinct genetic differences mechanisms enhance for different inducing cues. Interestingly, find increased activity has much more profound impact on H-NS-repressed, horizontally acquired genes than ancestral members PhoP regulon. We observe systems other bacteria exhibit levels similar E. system, suggesting are result Salmonella-specific adaptations produced active when encountering SCV conditions. Collectively, study offers window into evolutionary TCS enable it assume an expanded unique environment.

Language: Английский

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The two-component sensor factor envZ influences antibiotic resistance and virulence in the evolutionary dynamics of multidrug-resistant Salmonella enteritidis causing multisite invasive infections

Journal of Antimicrobial Chemotherapy, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 4, 2024

To assess the impact of mutations in two-component sensor envZ on antibiotic resistance and virulence evolutionary dynamics MDR Salmonella enteritidis (S. enteritidis).

Language: Английский

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