Unraveling the molecular mechanisms involved in plant growth promotion and soil bacterial community modulation by >i<Pantoea agglomerans>/i< 33.1 DOI Creative Commons

Aline Aparecida Oliveira Ferreira

Published: Dec. 7, 2023

Protein Secretion Systems (PSS) are essential molecular machineries to bacterial survival and biotic abiotic interactions.The PSS have been reported as an important system in beneficial plant/bacteria interactions, delivering substrates the host.Pantoea agglomerans 33.1 is a phosphate solubilizing bacterium, with capacity promote plant growth described for several crops.Considering agricultural potential of 33.1, comprehensive understanding its mechanisms vital.Thus, this research aimed investigate role promotion by through gene knockout via CRISPR-Cas9.To achieve knowledge, whole-genome was sequenced assembled, genes related main growth-promoting pathways were annotated, those mining manually compared found other P. strains.Based on annotation, two different selected CRISPR-Cas9, employing two-plasmid strategy.We obtained total genome 4,844,324 base-pairs (bp) divided into chromosome four plasmids, that harbor processes promotion, such P metabolism indole-3-acetic-acid (IAA) production.The classical secretion systems 1, 5a, 5b, 6, Sec Tat, appendages accessories Bam Complex T4P 33.1.Our prediction line what observed comparative genomics results, where 1 5 shown be most conserved among strains agglomerans.Concerning editing, we 33.1.:pCasstrain bepC prn properly cloned pTarget series.Despite required CRISPR-Cas9 correctly failed knock out Outer Membrane (OMP) Autotransporter (AT) from T1SS T5aSS, respectively.Regardless unsuccessful efforts, our provides insights about biology topics improved assays will continued group, until mutants obtained.

Language: Английский

Omics approaches in understanding the benefits of plant-microbe interactions DOI Creative Commons
Archana Jain, Surendra Sarsaiya,

Ranjan Singh

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 27, 2024

Plant-microbe interactions are pivotal for ecosystem dynamics and sustainable agriculture, influenced by various factors, such as host characteristics, environmental conditions, human activities. Omics technologies, including genomics, transcriptomics, proteomics, metabolomics, have revolutionized our understanding of these interactions. Genomics elucidates key genes, transcriptomics reveals gene expression dynamics, proteomics identifies essential proteins, metabolomics profiles small molecules, thereby offering a holistic perspective. This review synthesizes diverse microbial-plant interactions, showcasing the application omics in mechanisms, nitrogen fixation, systemic resistance induction, mycorrhizal association, pathogen-host Despite challenges data integration ethical considerations, approaches promise advancements precision intervention resilient agricultural practices. Future research should address challenges, enhance technology resolution, explore epigenomics, understand plant-microbe under conditions. In conclusion, technologies hold immense optimizing strategies fortifying alliances, paving way agriculture stewardship.

Language: Английский

Citations

18
Virulence Regulation in Plant-Pathogenic Bacteria by Host-Secreted Signals DOI Creative Commons
Muhammad Asif, Xin Xie, Zhibo Zhao

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 288, P. 127883 - 127883

Published: Aug. 24, 2024

Bacterial pathogens manipulate host signaling pathways and evade defenses using effector molecules, coordinating their deployment to ensure successful infection. However, host-derived metabolites as signals, critical role in regulating bacterial virulence requires further insights. Effective regulation of virulence, which is essential for pathogenic bacteria, involves controlling factors that enable colonization, defense evasion, tissue damage. This dynamic, influenced by environmental cues including signals from plants like exudates. Plant exudates, comprising diverse compounds released roots tissues, serve rich chemical affecting the behavior associated bacteria. nutrients act molecules are sensed through membrane-localized receptors intracellular response mechanisms review explains how different bacteria detect answer secreted gene expression. Our main emphasis exploring recognition process host-originated molecular sensors on cellular membranes pathways. encompasses insights into strains individually coordinate various distinct can positively or negatively regulate virulence. Furthermore, we explained interruption plant with perception dampen pathogen The intricate interplay between particularly recognize metabolic genes, portrays a crucial initial interaction leading profound influences infection outcomes. work will greatly aid researchers developing new strategies preventing treating infections.

Language: Английский

Citations

4
Bacterial targets of fecal host miRNAs in high-fat diet-fed mice DOI Creative Commons
Laila Silamiķele, Ivars Silamiķelis,

Patrīcija Paulīne Kotoviča

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0315871 - e0315871

Published: Feb. 11, 2025

The gut microbiome composition is intricately linked to the host’s health status, yet mechanisms underlying its interaction with host are not fully understood. MicroRNAs (miRNAs), facilitating intercellular communication, found in bodily fluids, including intestinal content, where they may affect microbiome. However, their role type 2 diabetes (T2D)-associated and treatment implications explored. Our study investigated how miRNAs influence changes related metformin a T2D mouse model. Analyzing fecal mucosal samples via small RNA sequencing, we correlated results sequencing data, identifying miRNA-microbiome correlations, bacterial targets, proteins targeted these bacteria. Significant differences miRNA expression based on diet location were noted, minor effects from proximal intestine of non-diabetic male mice. Key targeting bacteria included mmu-miR-5119, mmu-miR-5126, mmu-miR-6538, mmu-miR-2137, primarily affecting Oscillospiraceae_NOV, Lachnospiraceae_NOV, Bacteroides. analysis revealed diverse biological molecular effects. Further research into miRNA-bacteria interactions could lead new strategies for manipulating beyond.

Language: Английский

Citations

0
Contact-dependent antagonism is mediated by a T7SSb toxin effector–immunity protein pair via ADP-ribosylation DOI Creative Commons
Hongxin Guan, Huan Wu, Manrong Gan

et al.

Science Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Study on the influence mechanism of anionic polyacrylamide on acidogenic fermentation of chemically enhanced primary treatment sludge DOI

Lu Tian,

Lei Pan, Lin Wang

et al.

Journal of environmental chemical engineering, Journal Year: 2025, Volume and Issue: unknown, P. 116769 - 116769

Published: April 1, 2025

Language: Английский

Citations

0
The Passage of Chaperonins to Extracellular Locations in Legionella pneumophila Requires a Functional Dot/Icm System DOI Creative Commons
Peter K. J. Robertson, David Allan, Rafael A. Garduño

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(1), P. 91 - 91

Published: Jan. 9, 2025

HtpB, the chaperonin of bacterial pathogen L. pneumophila, is found in extracellular locations, even cytoplasm host cells. Although chaperonins have an essential cytoplasmic function protein folding, HtpB exits to perform virulence-related functions that support pneumophila’s lifestyle. The mechanism by which reaches locations not currently understood. To address this experimental gap, immunoelectron microscopy, trypsin-accessibility assays, and cell fractionation were used localize various pneumophila secretion mutants. Dot/Icm type IV mutants displayed less surface-exposed more periplasmic than parent strains. analysis extracts outer membrane vesicles these mutants, where co-localized with bona fide proteins, confirmed elevated levels HtpB. Genetic complementation recovered strain export GSK-tagged into infected cells was also Dot/Icm-dependent. translocating role system specific for because GroEL, Escherichia coli, at surface accumulated periplasm Dot when expressed pneumophila. These findings establish a functional required reach but remains partially unidentified.

Language: Английский

Citations

0
A lysis less ordinary: The bacterial Type 10 Secretion System DOI

M. A. R. Chowdhury,

Phillip J. Stansfeld, Frank Sargent

et al.

Advances in microbial physiology/Advances in Microbial Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Identification and characterization of Vibrio anguillarum (GA strain) isolated from Obscure Pufferfish Takifugu obscurus DOI
Wei Gao,

Wenji Huang,

Ling Shao

et al.

Journal of Aquatic Animal Health, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

ABSTRACT Objective Aquaculture has faced significant challenges due to the emergence of various pathogens affecting fish species. One such species, Obscure Pufferfish Takifugu obscurus, experienced high mortality rates an outbreak disease on a fishery farm in Shanghai. Methods The pathogen responsible for this was isolated and identified as Vibrio anguillarum (GA strain) using combination morphological, biochemical, whole-genome single-nucleotide polymorphism-based phylogenetic analyses. Pathogenicity tests confirmed that GA strain could cause healthy Pufferfish, inducing overt hemorrhagic symptoms. Histopathological analysis performed assess whether tissue damage had occurred. Whole-genome sequencing revealed possessed 235 virulence genes two drug resistance-related genes: cyclic AMP receptor protein (CRP) regulator secondary metabolites A (rsmA). Testing via PCR further presence 10 common genes. Results Antibiotic susceptibility testing showed highly sensitive antibiotics tetracycline, doxycycline, minocycline, compound sulfamethoxazole resistant cefradine, cefazolin, penicillin, vancomycin. Conclusions To best our knowledge, is first study report V. Pufferfish. isolate exhibited strong multidrug resistance. findings lay foundation control investigation epidemiological mechanisms anguillarum.

Language: Английский

Citations

0
Flowing boundaries in autopoietic systems and microniche construction DOI

Matěj Jureček,

Jana Švorcová

Biosystems, Journal Year: 2025, Volume and Issue: unknown, P. 105477 - 105477

Published: May 1, 2025

Language: Английский

Citations

0
The type II secretion system as an underappreciated and understudied mediator of interbacterial antagonism DOI
Nicholas P. Cianciotto

Infection and Immunity, Journal Year: 2024, Volume and Issue: 92(8)

Published: Aug. 13, 2024

ABSTRACT Interbacterial antagonism involves all major phyla, occurs across the full range of ecological niches, and has great significance for environment, clinical arena, agricultural industrial sectors. Though earliest insight into interbacterial traces back to discovery antibiotics, a paradigm shift happened when it was learned that protein secretion systems (e.g., types VI IV systems) deliver toxic “effectors” against competitors. However, link between Gram-negative type II system (T2SS), which exists in many pathogens environmental species, is not evident prior reviews on bacterial competition or T2SS function. A current examination literature revealed four examples one its known substrates having bactericidal activity Gram-positive target another Gram-negative. When further studied, effectors proved be peptidases peptidoglycan competitor. There are also reports various bacteriolytic enzymes occurring culture supernatants some other these activities suggested. Thus, can mediator antagonism, possible T2SSs have antibacterial outputs. Yet, at present, remains relatively understudied role competition. Arguably, there need analyze broader species their antagonism. Such investigation offers, among things, pathway toward developing new antimicrobials treating disease.

Language: Английский

Citations

2