Pseudomonas aeruginosa PfpI is a methylglyoxalase
Journal of Biological Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown, P. 108374 - 108374
Published: March 1, 2025
Pseudomonas
aeruginosa
is
an
opportunistic
pathogen,
commonly
associated
with
human
airway
infections.
Based
on
its
amino
acid
sequence
similarity
Pyrococcus
furiosusprotease
I,
P.
PfpI
was
originally
annotated
as
intracellular
protease.
In
this
work,
we
show
that
a
methylglyoxalase.
The
X-ray
crystal
structure
of
the
purified
protein
solved
to
1.4
Å
resolution.
structural
data
indicated
shares
same
constellation
active
site
residues
(including
catalytic
Cys112
and
His113)
those
seen
in
well-characterized
bacterial
methylglyoxalase
from
Escherichia
coli,
YhbO.
Using
NMR,
confirmed
qualitatively
converted
methylglyoxal
into
lactic
acid.
Quantitation
lactate
produced
by
activity
yielded
kcat
102
min-1
KM
369
μM.
Mutation
His113
led
complete
loss
activity.
To
investigate
functional
impact
vivo,
ΔpfpI
deletion
mutant
made.
Quantitative
proteomic
analyses
revealed
pattern
changes
consistent
perturbation
ribosomal
function,
Zn2+
limitation,
C1
metabolism,
glutathione
metabolism.
These
findings
are
being
glutathione-independent
Previously,
transposon
insertion
(pfpI::Tn)
mutants
have
been
reported
exhibit
phenotypes
antibiotic
resistance,
motility
response
oxidative
stress.
However,
generated
study
displayed
none
these
phenotypes.
Whole-genome
sequencing
previously
described
pfpI::Tn
they
also
contain
variety
other
genetic
likely
account
for
their
observed
Language: Английский
Pathogenicity and virulence of Pseudomonas aeruginosa : Recent advances and under-investigated topics.
Jemima E. V. Swain,
No information about this author
Isabel Askenasy,
No information about this author
Rahan Rudland Nazeer
No information about this author
et al.
Virulence,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 12, 2025
Pseudomonas
aeruginosa
is
a
model
for
the
study
of
quorum
sensing,
protein
secretion,
and
biofilm
formation.
Consequently,
it
has
become
one
most
intensely
reviewed
pathogens,
with
many
excellent
articles
in
current
literature
focusing
on
these
aspects
organism's
biology.
Here
though,
we
aim
to
take
slightly
different
approach
consider
some
less
well
appreciated
(but
nonetheless
important)
factors
that
affect
P.
virulence.
We
start
by
reminding
reader
global
importance
infection,
"virulome"
very
niche
-
specific.
Overlooked
but
obvious
questions
such
as
"what
prevents
secreted
products
from
being
digested
co-secreted
proteases?"
are
discussed,
suggest
how
nutritional
preference(s)
organism
might
dictate
its
environmental
reservoirs.
Recent
studies
identifying
host
genes
associated
genetic
predisposition
towards
infection
(and
even
specific
strains),
role(s)
intracellular
introduced.
also
discuss
fact
virulence
high-risk
strategy,
touch
expression
two
main
classes
factor
regulated.
A
particular
focus
recent
findings
highlighting
status
metabolism
important
sensing
terms
their
impact
virulence,
co-habiting
microbial
species
at
site
vice
versa).
It
our
view
investigation
issues
likely
dominate
research
into
this
WHO-designated
priority
pathogen
over
next
decade.
Language: Английский