Pseudomonas aeruginosa PfpI is a methylglyoxalase

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108374 - 108374

Published: March 1, 2025

Pseudomonas aeruginosa is an opportunistic pathogen, commonly associated with human airway infections. Based on its amino acid sequence similarity Pyrococcus furiosusprotease I, P. PfpI was originally annotated as intracellular protease. In this work, we show that a methylglyoxalase. The X-ray crystal structure of the purified protein solved to 1.4 Å resolution. structural data indicated shares same constellation active site residues (including catalytic Cys112 and His113) those seen in well-characterized bacterial methylglyoxalase from Escherichia coli, YhbO. Using NMR, confirmed qualitatively converted methylglyoxal into lactic acid. Quantitation lactate produced by activity yielded kcat 102 min-1 KM 369 μM. Mutation His113 led complete loss activity. To investigate functional impact vivo, ΔpfpI deletion mutant made. Quantitative proteomic analyses revealed pattern changes consistent perturbation ribosomal function, Zn2+ limitation, C1 metabolism, glutathione metabolism. These findings are being glutathione-independent Previously, transposon insertion (pfpI::Tn) mutants have been reported exhibit phenotypes antibiotic resistance, motility response oxidative stress. However, generated study displayed none these phenotypes. Whole-genome sequencing previously described pfpI::Tn they also contain variety other genetic likely account for their observed

Language: Английский

Pathogenicity and virulence of Pseudomonas aeruginosa : Recent advances and under-investigated topics.

Virulence, Journal Year: 2025, Volume and Issue: unknown

Published: May 12, 2025

Pseudomonas aeruginosa is a model for the study of quorum sensing, protein secretion, and biofilm formation. Consequently, it has become one most intensely reviewed pathogens, with many excellent articles in current literature focusing on these aspects organism's biology. Here though, we aim to take slightly different approach consider some less well appreciated (but nonetheless important) factors that affect P. virulence. We start by reminding reader global importance infection, "virulome" very niche - specific. Overlooked but obvious questions such as "what prevents secreted products from being digested co-secreted proteases?" are discussed, suggest how nutritional preference(s) organism might dictate its environmental reservoirs. Recent studies identifying host genes associated genetic predisposition towards infection (and even specific strains), role(s) intracellular introduced. also discuss fact virulence high-risk strategy, touch expression two main classes factor regulated. A particular focus recent findings highlighting status metabolism important sensing terms their impact virulence, co-habiting microbial species at site vice versa). It our view investigation issues likely dominate research into this WHO-designated priority pathogen over next decade.

Language: Английский