Neural connectivity molecules best identify the heterogeneous clock and dopaminergic cell types in theDrosophilaadult brain DOI Creative Commons
Dingbang Ma, Nicholas Herndon, Jasmine Quynh Le

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: April 22, 2022

Abstract Our recent single cell sequencing of most adult Drosophila circadian neurons indicated striking gene expression heterogeneity, about 2-3 cells per clock neuron group. To extend this characterization to other fly brain neurons, we used the identical plate-based methods generate data from a similar subset dopaminergic neurons. minimize batch effects and apply an additional strategy, also assayed these two populations together with 10X Chromium. An unsupervised clustering algorithm indicates that are comparably heterogeneous, suggesting transcriptomic diversity parallels its EM connectome. The results here further indicate connectivity molecules like surface best characterize all groups. We suggest surprising features general make major contributions neuronal identity central as well underlie complex behavioral repertoire .

Language: Английский

Endocrine cybernetics: neuropeptides as molecular switches in behavioural decisions DOI Creative Commons
Dick R. Nässel, Meet Zandawala

Open Biology, Journal Year: 2022, Volume and Issue: 12(7)

Published: July 1, 2022

Plasticity in animal behaviour relies on the ability to integrate external and internal cues from changing environment hence modulate activity synaptic circuits of brain. This context-dependent neuromodulation is largely based non-synaptic signalling with neuropeptides. Here, we describe select peptidergic systems Drosophila brain that act at different levels a hierarchy associated physiology. These regions, such as central complex mushroom bodies, which supervise specific behaviours. At top level there are small numbers large neurons arborize widely multiple areas orchestrate or global state manner. bottom local provide executive sensory gain intrinsically restricted parts neuronal circuits. The orchestrating receive interoceptive signals mediate energy sleep homeostasis, metabolic circadian timing, well affect food search, aggression mating. Some these can be triggers conflicting behaviours mating versus aggression, feeding, participate circuits, enabling choices switches.

Language: Английский

Citations

56
Neural connectivity molecules best identify the heterogeneous clock and dopaminergic cell types in theDrosophilaadult brain DOI Creative Commons
Dingbang Ma, Nicholas Herndon, Jasmine Quynh Le

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: April 22, 2022

Abstract Our recent single cell sequencing of most adult Drosophila circadian neurons indicated striking gene expression heterogeneity, about 2-3 cells per clock neuron group. To extend this characterization to other fly brain neurons, we used the identical plate-based methods generate data from a similar subset dopaminergic neurons. minimize batch effects and apply an additional strategy, also assayed these two populations together with 10X Chromium. An unsupervised clustering algorithm indicates that are comparably heterogeneous, suggesting transcriptomic diversity parallels its EM connectome. The results here further indicate connectivity molecules like surface best characterize all groups. We suggest surprising features general make major contributions neuronal identity central as well underlie complex behavioral repertoire .

Language: Английский

Citations

0