Gene drive and genetic sex conversion in the global agricultural pestCeratitis capitata DOI Creative Commons
Angela Meccariello, Shibo Hou, Serafima Davydova

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 17, 2023

Abstract Homing-based gene drives are novel interventions promising the area-wide, species-specific genetic control of harmful insect populations. Here we characterise a first set in tephritid agricultural pest species, Mediterranean fruit fly Ceratitis capitata (medfly). Our results show that medfly is highly amenable to homing-based drive strategies. By targeting transformer gene, also demonstrate two different mechanisms by which CRISPR-Cas9 can be coupled sex conversion, whereby females transformed into fertile and harmless XX males. Given this unique malleability determination, modelled couple conversion female sterility found such approaches could effective tolerant resistant allele selection target population. open door for developing strains population suppression related pests co-targeting reproduction shifting reproductive ratio towards They untapped potential tackle an environmentally friendly economical way.

Language: Английский

Gene drive and genetic sex conversion in the global agricultural pest Ceratitis capitata DOI Creative Commons
Angela Meccariello, Shibo Hou, Serafima Davydova

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 8, 2024

Homing-based gene drives are recently proposed interventions promising the area-wide, species-specific genetic control of harmful insect populations. Here we characterise a first set in tephritid agricultural pest species, Mediterranean fruit fly Ceratitis capitata (medfly). Our results show that medfly is highly amenable to homing-based drive strategies. By targeting transformer gene, also demonstrate how CRISPR-Cas9 can be coupled sex conversion, whereby females transformed into fertile and harmless XX males. Given this unique malleability determination, modelled couple conversion female sterility found such approaches could effective tolerant resistant allele selection target population. open door for developing strains population suppression related pests by co-targeting reproduction shifting reproductive ratio towards They untapped potential tackle an environmentally friendly economical way.

Language: Английский

Citations

13
Gene drive and genetic sex conversion in the global agricultural pestCeratitis capitata DOI Creative Commons
Angela Meccariello, Shibo Hou, Serafima Davydova

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 17, 2023

Abstract Homing-based gene drives are novel interventions promising the area-wide, species-specific genetic control of harmful insect populations. Here we characterise a first set in tephritid agricultural pest species, Mediterranean fruit fly Ceratitis capitata (medfly). Our results show that medfly is highly amenable to homing-based drive strategies. By targeting transformer gene, also demonstrate two different mechanisms by which CRISPR-Cas9 can be coupled sex conversion, whereby females transformed into fertile and harmless XX males. Given this unique malleability determination, modelled couple conversion female sterility found such approaches could effective tolerant resistant allele selection target population. open door for developing strains population suppression related pests co-targeting reproduction shifting reproductive ratio towards They untapped potential tackle an environmentally friendly economical way.

Language: Английский

Citations

5