Super Bowl, an open platform for visual stimulation of insects DOI Creative Commons
Stefan Prech, Lukas N. Groschner, Alexander Borst

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 6, 2023

Abstract To study how the nervous system processes visual information, experimenters must record neural activity while delivering stimuli in a controlled fashion. In animals with nearly panoramic field of view, such as flies, precise stimulation entire is challenging. We describe projector-based device for insect under microscope. The based on bowl-shaped screen that provides wide and distortion-free view. It compact, cheap, easy to assemble, operate using included open-source software stimulus generation. validate virtual reality technically demonstrate its capabilities series experiments at two levels: cellular, by measuring membrane potential responses interneurons; organismal, recording optomotor fixation behavior Drosophila melanogaster tethered flight. Our reveal importance stimulating an we provide simple solution do so.

Language: Английский

Small-field visual projection neurons detect translational optic flow and support walking control DOI Creative Commons
Matthew Isaacson, Jessica Eliason, Aljoscha Nern

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 22, 2023

SUMMARY Animals rely on visual motion for navigating the world, and research in flies has clarified how neural circuits extract information from moving scenes. However, major pathways connecting these patterns of optic flow to behavior remain poorly understood. Using a high-throughput quantitative assay visually guided behaviors genetic neuronal silencing, we discovered region Drosophila ’s protocerebrum critical following. We used calcium imaging, optogenetics identify single cell type, LPC1, that innervates this region, detects translational flow, plays key role regulating forward walking. Moreover, population LPC1s can estimate travelling direction, such as when gaze direction diverges body heading. By linking specific types their computations behaviors, our findings establish foundation understanding nervous system uses vision guide navigation.

Language: Английский

Citations

26
Eye structure shapes neuron function inDrosophilamotion vision DOI Creative Commons
Arthur Zhao, Eyal Gruntman, Aljoscha Nern

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Dec. 15, 2022

Summary Many animals rely on vision to navigate through their environment. The pattern of changes in the visual scene induced by self-motion is optic flow 1 , which first estimated local patches directionally selective (DS) neurons 2–4 . But how should arrays DS neurons, each responsive motion a preferred direction at specific retinal position, be organized support robust decoding downstream circuits? Understanding this global organization challenging because it requires mapping fine, features across animal’s field view 3 In Drosophila asymmetric dendrites T4 and T5 establish direction, making possible predict responses from anatomy 4,5 Here we report that directions fly vary different positions show spatial variation established compound eye. To estimate field, reconstructed hundreds full brain EM volume 6 discovered unexpectedly stereotypical dendritic arborizations are independent location. We then used whole-head μCT scans map viewing all eye facets found non-uniform sampling space explains directions. Our findings largely determined eye, exposing an intimate unexpected connection between peripheral structure functional properties deep brain, control body movements.

Language: Английский

Citations

28
A competitive disinhibitory network for robust optic flow processing in Drosophila DOI Creative Commons
Mert Erginkaya, Tomás Cruz, Margarida Brotas

et al.

Nature Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
An open platform for visual stimulation of insects DOI Creative Commons
Stefan Prech, Lukas N. Groschner, Alexander Borst

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(4), P. e0301999 - e0301999

Published: April 18, 2024

To study how the nervous system processes visual information, experimenters must record neural activity while delivering stimuli in a controlled fashion. In animals with nearly panoramic field of view, such as flies, precise stimulation entire is challenging. We describe projector-based device for insect under microscope. The based on bowl-shaped screen that provides wide and distortion-free view. It compact, cheap, easy to assemble, operate using included open-source software stimulus generation. validate virtual reality technically demonstrate its capabilities series experiments at two levels: cellular, by measuring membrane potential responses interneurons; organismal, recording optomotor fixation behavior Drosophila melanogaster tethered flight. Our reveal importance stimulating an we provide simple solution do so.

Language: Английский

Citations

1
Speed-Independent Wall Distance Estimation Along a Given Trajectory of a Biohybrid Fly-Robot-Interface DOI
Jiaqi Huang, Holger G. Krapp

Lecture notes in computer science, Journal Year: 2024, Volume and Issue: unknown, P. 413 - 427

Published: Dec. 12, 2024

Language: Английский

Citations

0
Super Bowl, an open platform for visual stimulation of insects DOI Creative Commons
Stefan Prech, Lukas N. Groschner, Alexander Borst

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 6, 2023

Abstract To study how the nervous system processes visual information, experimenters must record neural activity while delivering stimuli in a controlled fashion. In animals with nearly panoramic field of view, such as flies, precise stimulation entire is challenging. We describe projector-based device for insect under microscope. The based on bowl-shaped screen that provides wide and distortion-free view. It compact, cheap, easy to assemble, operate using included open-source software stimulus generation. validate virtual reality technically demonstrate its capabilities series experiments at two levels: cellular, by measuring membrane potential responses interneurons; organismal, recording optomotor fixation behavior Drosophila melanogaster tethered flight. Our reveal importance stimulating an we provide simple solution do so.

Language: Английский

Citations

0