Molecular logic of neuromodulatory systems in the zebrafish telencephalon DOI Creative Commons
Lukas Anneser, Chie Satou, Hans-Rudolf Hotz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 5, 2023

Abstract The function of neuronal networks is determined not only by synaptic connectivity but also neuromodulatory systems that broadcast information via distributed connections and volume transmission. To understand the molecular constraints organize signaling in telencephalon adult zebrafish we used transcriptomics additional approaches to delineate cell types, determine their phylogenetic conservation, map expression marker genes at high granularity. combinatorial GPCRs type markers indicates all types are subject modulation multiple monoaminergic distinct combinations neuropeptides. Individual were associated with (typically >30) expressed a few diagnostic levels, suggesting different act combination albeit unequal weights. These results provide detailed brain areas telencephalon, identify core components networks, highlight type-specificity neuropeptides GPCRs, begin decipher logic neuromodulation.

Language: Английский

A molecular atlas of adultC. elegansmotor neurons reveals ancient diversity delineated by conserved transcription factor codes DOI Creative Commons
Jayson J. Smith, Seth R. Taylor, Jacob A. Blum

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 6, 2023

Motor neurons (MNs) constitute an ancient cell type targeted by multiple adult-onset diseases. It is therefore important to define the molecular makeup of adult MNs in animal models and extract organizing principles. Here, we generated a comprehensive atlas

Language: Английский

Citations

1
Scaling Properties for Artificial Neural Network Models of a Small Nervous System DOI Creative Commons
Quilee Simeon, Leandro Venâncio, Michael Skuhersky

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 14, 2024

Abstract The nematode worm C. elegans provides a unique opportunity for exploring in silico data-driven models of whole nervous system, given its transparency and well-characterized system facilitating wealth measurement data from wet-lab experiments. This study explores the scaling properties that may govern learning underlying neural dynamics this small by using artificial network (ANN) models. We investigate accuracy self-supervised next time-step activity prediction as function For scaling, we report monotonic log-linear reduction mean-squared error (MSE) amount data. model find MSE to be nonlinear size ANN Furthermore, observe dataset are influenced particular choice architecture but not precise experimental source Our results fall short producing long-horizon predictive generative suggest directions achieve those. In our extrapolate recording more is fruitful near-term approach obtaining better system.

Language: Английский

Citations

0
Functional analysis of conserved C. elegans bHLH family members uncovers lifespan control by a peptidergic hub neuron DOI
G. Robert Aguilar,

Berta Vidal,

Hongzhu Ji

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 16, 2024

ABSTRACT Throughout the animal kingdom, several members of basic helix-loop-helix (bHLH) family act as proneural genes during early steps nervous system development. Roles bHLH in specifying terminal differentiation postmitotic neurons have been less extensively studied. We analyze here function five C. elegans genes, falling into three phylogenetically conserved subfamilies, which are continuously expressed a very small number central system. show (a) that two orthologs vertebrate bHLHb4/b5 called hlh-17 and hlh-32, redundantly to specify identity single head interneuron (AUA), well an individual motor neuron (VB2), (b) PTF1a ortholog hlh-13 acts selector control sole octopaminergic class , RIC, (c) NHLH1/2 hlh-15 controls peptidergic AVK class, known neuropeptidergic signaling hub animal. Strikingly, through null mutant analysis cell-specific rescue experiments, we find loss hlh-15/NHLH resulting abrogation neuropeptide secretion causes substantially expanded lifespan animal, revealing unanticipated impact central, regulating lifespan, propose be akin hypothalamic vertebrates. Taken together, our functional reveals themes gene complementary earlier lineage specification roles other members. However, such late functions much more sparsely employed by transcription factor family, compared broadly homeodomain family.

Language: Английский

Citations

0
A tonically active master neuron modulates mutually exclusive motor states at two timescales DOI Creative Commons
Jun Meng, Tosif Ahamed, Bin Yu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: April 9, 2022

Abstract Continuity of behaviors requires animals to make smooth transitions between successive and mutually exclusive behavioral states. Neural principles that govern these are not well understood. C. elegans spontaneously switch two opposite motor states, forward backward movement, a phenomenon long thought reflect the reciprocal inhibition interneurons separately gate circuits, AVB AVA. Combining experimental data mathematical modeling, we report spontaneous locomotion their corresponding circuits controlled. AVA neither functionally equivalent nor strictly reciprocally inhibitory. Instead, while phasically inhibits promoting interneuron at fast timescale, it maintains tonic, extrasynaptic excitation on over longer timescale. AVA’s depolarized membrane potential is necessary for this tonic excitation. We propose new, master neuron model locomotion. AVA, with phasic activity polarities different time scales, acts as break symmetry underlying circuits. This offers parsimonious solution sustained consisted Teaser A tonically active premotor functions underlies continuous modulation movement ensure opposing

Language: Английский

Citations

2
Molecular logic of neuromodulatory systems in the zebrafish telencephalon DOI Creative Commons
Lukas Anneser, Chie Satou, Hans-Rudolf Hotz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 5, 2023

Abstract The function of neuronal networks is determined not only by synaptic connectivity but also neuromodulatory systems that broadcast information via distributed connections and volume transmission. To understand the molecular constraints organize signaling in telencephalon adult zebrafish we used transcriptomics additional approaches to delineate cell types, determine their phylogenetic conservation, map expression marker genes at high granularity. combinatorial GPCRs type markers indicates all types are subject modulation multiple monoaminergic distinct combinations neuropeptides. Individual were associated with (typically >30) expressed a few diagnostic levels, suggesting different act combination albeit unequal weights. These results provide detailed brain areas telencephalon, identify core components networks, highlight type-specificity neuropeptides GPCRs, begin decipher logic neuromodulation.

Language: Английский

Citations

0