Distinct roles of H3K27me3 and H3K36me3 in vernalization response, maintenance and resetting in winter wheat DOI Open Access
Xuemei Liu, Xuelei Lin, Min Deng

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 20, 2023

Abstract Winter plants rely on vernalization, a vital process for adapting to cold and ensuring successful reproduction. However, understanding the role of histone modifications in guiding vernalization winter wheat is limited. In this study, we investigate transcriptome chromatin dynamics shoot apex throughout life cycle field. Two core modifications, H3K27me3 H3K36me3, exhibit opposite pattern key gene VERNALIZATION1 ( VRN1 ), correlated with its induction during exposure. Additionally, H3K36me3 remains high at after exposure, maintaining active state. Mutations FERTILIZATION-INDEPENDENT ENDOSPERM (TaFIE) SET DOMAIN GROUP 8 (TaSDG8), writer complex components respectively, affect flowering time. Interestingly, loses expression exposure memory absence H3K36me3. During embryo development, silenced removal both spring alleles. selectively added allele, influencing requirement next generation. Integrating patterns identified potential regulators flowering. This study reveals distinct roles controlling response, maintenance, resetting wheat. Significance Statement Vernalization, initially observed cereals, lacks comprehensive underlying mechanism, particularly regarding chromatin-mediated transcriptional regulation By delving into wheat’s cycle, pinpointed two crucial each playing different stages. implicated establishing extended wheat, gradually diminishing vernalization. On other hand, ’s state post-cold contributing treatment. integration modification profiles unveiled novel

Language: Английский

Unraveling wheat endosperm development: epigenetic regulation and novel regulators for enhanced yield and quality DOI Open Access
Long Zhao, Jinchao Chen,

Zhaoheng Zhang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 8, 2024

Starch content and seed storage protein (SSP) composition are critical factors influencing wheat grain yield quality. To uncover the molecular mechanisms governing their biosynthesis, we conducted transcriptome epigenome profiling across key endosperm developmental stages, revealing that chromatin accessibility, H3K27ac, H3K27me3 collectively regulate SSP starch genes with varying impact. Population phenotype analyses highlighted crucial role of accessible promoter regions as a genetic variation resource, quality in core collection accessions. By integrating time-serial RNA-seq ATAC-seq data, constructed hierarchical transcriptional regulatory network (TRN) identifying 42 high-confidence novel candidates. These candidates exhibited overlap associated size traits, functional significance was validated through expression-phenotype association analysis among accessions TILLING mutants. In-depth abscisic acid insensitive 3-A1 (TaABI3-A1) genome editing knock-out lines demonstrated its promoting accumulation while repressing biosynthesis regulation. An elite haplotype TaABI3-A1 higher weight identified during breeding process China, superior trait altered expression levels. Additionally, potential upstream regulator, GAGA-binding transcription factor 1 (TaGBP1), expression. Our study provides regulators, presenting an effective strategy for understanding regulation contributing to enhancement.

Language: Английский

Citations

2
Identification and validation of new quantitative trait loci for spike-related traits in two RIL populations DOI
Yuying Wang,

Zhankui Zeng,

Jiachuang Li

et al.

Molecular Breeding, Journal Year: 2023, Volume and Issue: 43(8)

Published: July 31, 2023

Language: Английский

Citations

1
Boosting wheat functional genomics via indexed EMS mutant library of KN9204 DOI Creative Commons
Dongzhi Wang, Yongpeng Li, Haojie Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Dec. 8, 2022

Summary A better understanding of wheat functional genomics could facilitate the targeted breeding for agronomic traits improvement and environmental adaptation. With release reference genomes extensive re-sequencing data relatives, enters a new era. However, limited transformation efficiency in hampers in-depth gene study genetic manipulation breeding. Here, we generated an EMS mutagenesis library KN9204, widely grown elite variety northern China, with available genome, transcriptome, epigenome various tissues. The harbors enormous developmental diversity covering important tissues transition stages. Exome capture sequencing 2,090 mutant lines, probes designed by KN9204 revealed that 98.79% coding genes have mutations 1,383 EMS-type SNPs per line averagely. Novel allelic variations trait-related genes, such as Rht-D1, Q, TaTB1 , WFZP were identified. We tested 100 lines severe 80 NAC TFs under drought salinity stresses, found 13 altered sensitivity. Three further analyzed regulation insight stress response combing transcriptome chromatin accessibility data. Hundreds direct targets transcriptional pattern salt or induction identified, including SNAC1, DREB2B, CML16 ZFP182 knowing factors abiotic stresses response. Thus, indexed which would provide resources wheat.

Language: Английский

Citations

1
Distinct roles of H3K27me3 and H3K36me3 in vernalization response, maintenance and resetting in winter wheat DOI Open Access
Xuemei Liu, Xuelei Lin, Min Deng

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 20, 2023

Abstract Winter plants rely on vernalization, a vital process for adapting to cold and ensuring successful reproduction. However, understanding the role of histone modifications in guiding vernalization winter wheat is limited. In this study, we investigate transcriptome chromatin dynamics shoot apex throughout life cycle field. Two core modifications, H3K27me3 H3K36me3, exhibit opposite pattern key gene VERNALIZATION1 ( VRN1 ), correlated with its induction during exposure. Additionally, H3K36me3 remains high at after exposure, maintaining active state. Mutations FERTILIZATION-INDEPENDENT ENDOSPERM (TaFIE) SET DOMAIN GROUP 8 (TaSDG8), writer complex components respectively, affect flowering time. Interestingly, loses expression exposure memory absence H3K36me3. During embryo development, silenced removal both spring alleles. selectively added allele, influencing requirement next generation. Integrating patterns identified potential regulators flowering. This study reveals distinct roles controlling response, maintenance, resetting wheat. Significance Statement Vernalization, initially observed cereals, lacks comprehensive underlying mechanism, particularly regarding chromatin-mediated transcriptional regulation By delving into wheat’s cycle, pinpointed two crucial each playing different stages. implicated establishing extended wheat, gradually diminishing vernalization. On other hand, ’s state post-cold contributing treatment. integration modification profiles unveiled novel

Language: Английский

Citations

0