Bulk and Mosaic Deletions ofEgfrReveal Regionally Defined Gliogenesis in the Developing Mouse Forebrain DOI Open Access
Xuying Zhang,

Guanxi Xiao,

Caroline A. Johnson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Dec. 11, 2022

Summary The Epidermal growth factor receptor (EGFR) plays a role in cell proliferation and differentiation during healthy development tumor growth, however its requirement for brain remains unclear. Here we used conditional mouse allele Egfr to examine contributions perinatal forebrain at the tissue level. Subtractive bulk ventral dorsal deletions of uncovered significant permanent decreases oligodendrogenesis myelination cortex corpus callosum. Additionally, an increase astrogenesis or reactive astrocytes effected regions was evident response cortical scarring. Sparse deletion using Mosaic Analysis with Double Markers (MADM) surprisingly revealed regional EGFR rostrodorsal, but not ventrocaudal glial lineages including both oligodendrocytes. EGFR-independent progenitors may compensate missing EGFR-dependent glia -deleted forebrain, potentially exposing regenerative population gliogenic forebrain.

Language: Английский

Bulk and mosaic deletions of Egfr reveal regionally defined gliogenesis in the developing mouse forebrain DOI Creative Commons
Xuying Zhang,

Guanxi Xiao,

Caroline A. Johnson

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(3), P. 106242 - 106242

Published: Feb. 20, 2023

The epidermal growth factor receptor (EGFR) plays a role in cell proliferation and differentiation during healthy development tumor growth; however, its requirement for brain remains unclear. Here we used conditional mouse allele Egfr to examine contributions perinatal forebrain at the tissue level. Subtractive bulk ventral dorsal deletions of uncovered significant permanent decreases oligodendrogenesis myelination cortex corpus callosum. Additionally, an increase astrogenesis or reactive astrocytes effected regions was evident response cortical scarring. Sparse deletion using mosaic analysis with double markers (MADM) surprisingly revealed regional EGFR rostrodorsal, but not ventrocaudal glial lineages including both oligodendrocytes. EGFR-independent progenitors may compensate missing EGFR-dependent glia Egfr-deleted forebrain, potentially exposing regenerative population gliogenic forebrain.

Language: Английский

Citations

13
Bulk and Mosaic Deletions ofEgfrReveal Regionally Defined Gliogenesis in the Developing Mouse Forebrain DOI Open Access
Xuying Zhang,

Guanxi Xiao,

Caroline A. Johnson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Dec. 11, 2022

Summary The Epidermal growth factor receptor (EGFR) plays a role in cell proliferation and differentiation during healthy development tumor growth, however its requirement for brain remains unclear. Here we used conditional mouse allele Egfr to examine contributions perinatal forebrain at the tissue level. Subtractive bulk ventral dorsal deletions of uncovered significant permanent decreases oligodendrogenesis myelination cortex corpus callosum. Additionally, an increase astrogenesis or reactive astrocytes effected regions was evident response cortical scarring. Sparse deletion using Mosaic Analysis with Double Markers (MADM) surprisingly revealed regional EGFR rostrodorsal, but not ventrocaudal glial lineages including both oligodendrocytes. EGFR-independent progenitors may compensate missing EGFR-dependent glia -deleted forebrain, potentially exposing regenerative population gliogenic forebrain.

Language: Английский

Citations

0