Heterotrophic bacteria trigger transcriptome remodelling in the photosynthetic picoeukaryote Micromonas commoda DOI Creative Commons
Maria Hamilton, Frank Ferrer-González, Mary Ann Moran

et al.

Environmental Microbiology Reports, Journal Year: 2024, Volume and Issue: 16(3)

Published: May 22, 2024

Abstract Marine biogeochemical cycles are built on interactions between surface ocean microbes, particularly those connecting phytoplankton primary producers to heterotrophic bacteria. Details of these associations not well understood, especially in the case direct influences bacteria physiology. Here we catalogue how presence three marine ( Ruegeria pomeroyi DSS‐3, Stenotrophomonas sp. SKA14 and Polaribacter dokdonensis MED152) individually uniquely impact gene expression picoeukaryotic alga Micromonas commoda RCC 299. We find a dramatic transcriptomic remodelling by M. after 8 h co‐culture, followed an increase cell numbers 56 compared with axenic cultures. Some aspects algal response conserved across all bacterial co‐cultures, including unexpected reduction relative photosynthesis carbon fixation pathways. Expression differences restricted single bacterium also observed, Flavobacteriia P. eliciting changes genes involved biotin biosynthesis acquisition assimilation nitrogen. This study reveals that has rapid extensive responses ways generalizable, as taxon specific manner, implications for diversity phytoplankton‐bacteria ongoing ocean.

Language: Английский

Seasonal exometabolites are regulated by essential microbial metabolisms in the oligotrophic ocean DOI Open Access
Erin L. McParland, Fabian Wittmers, Luis M. Bolaños

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 5, 2024

Predictions of how the biogeochemical reservoir marine dissolved organic matter (DOM) will respond to future ocean changes require an improved understanding thousands individual microbe-molecule interactions which regulate transformation and fate DOM. Bulk characterizations can mask this complex network comprised rich chemical taxonomic diversity. Here, we present a three-year, depth-resolved time-series seasonal dynamics exometabolome bacterioplankton community at Bermuda Atlantic Time-series Study (BATS) site. We find both be highly structured compositionally distinct across sampling depths. Putative exometabolite identifications (gonyol, glucose 6-sulfate, succinate, trehalose) indicate that least portion contains rapidly remineralized, labile molecules. hypothesize apparent accumulation these molecules could result from environmental conditions alter composition on timescale thus shift relative proportions microbial functions produce consume substrates. Critically, found DOM features was more stable interannually than structure. By estimating redundancy metabolic responsible for cycling in BATS metagenomes, propose paradigm whereby core metabolisms, either those utilized by all or subset microbes, are better predictors taxonomies. The molecular-level characterization achieved herein highlights imprint activity greatly enhances our regulating largest carbon Earth.

Language: Английский

Citations

1
Heterotrophic bacteria trigger transcriptome remodelling in the photosynthetic picoeukaryote Micromonas commoda DOI Creative Commons
Maria Hamilton, Frank Ferrer-González, Mary Ann Moran

et al.

Environmental Microbiology Reports, Journal Year: 2024, Volume and Issue: 16(3)

Published: May 22, 2024

Abstract Marine biogeochemical cycles are built on interactions between surface ocean microbes, particularly those connecting phytoplankton primary producers to heterotrophic bacteria. Details of these associations not well understood, especially in the case direct influences bacteria physiology. Here we catalogue how presence three marine ( Ruegeria pomeroyi DSS‐3, Stenotrophomonas sp. SKA14 and Polaribacter dokdonensis MED152) individually uniquely impact gene expression picoeukaryotic alga Micromonas commoda RCC 299. We find a dramatic transcriptomic remodelling by M. after 8 h co‐culture, followed an increase cell numbers 56 compared with axenic cultures. Some aspects algal response conserved across all bacterial co‐cultures, including unexpected reduction relative photosynthesis carbon fixation pathways. Expression differences restricted single bacterium also observed, Flavobacteriia P. eliciting changes genes involved biotin biosynthesis acquisition assimilation nitrogen. This study reveals that has rapid extensive responses ways generalizable, as taxon specific manner, implications for diversity phytoplankton‐bacteria ongoing ocean.

Language: Английский

Citations

1