Impact of whole-genome duplications on structural variant evolution in Cochlearia

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 25, 2024

Abstract Polyploidy, the result of whole-genome duplication (WGD), is a major driver eukaryote evolution. Yet WGDs are hugely disruptive mutations, and we still lack clear understanding their fitness consequences. Here, study whether in greater diversity genomic structural variants (SVs) how they influence evolutionary dynamics plant genus, Cochlearia (Brassicaceae). By using long-read sequencing graph-based pangenome, find both negative positive interactions between SVs. Masking recessive mutations due to leads progressive accumulation deleterious SVs across four ploidal levels (from diploids octoploids), likely reducing adaptive potential polyploid populations. However, also discover putative benefits arising from SV accumulation, as more ploidy-specific harbor signals local adaptation polyploids than diploids. Together, our results suggest that play diverse contrasting roles trajectories young polyploids.

Language: Английский

---

Repeated colonisation of alpine habitats by Arabidopsis arenosa involved parallel adjustments of leaf cuticle traits

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Summary Cuticle function can be pivotal to plant success in different environments. Yet, the occurrence of intraspecific adjustments cuticle traits resulting from acclimation or adaptation habitats remains poorly understood. Here, we used genetically well‐characterised populations Arabidopsis arenosa investigate whether were adjusted as part parallel evolution a foothill an alpine ecotype. Six and six populations, representing at least three independent evolutionary origins ecotype, reciprocal transplantation experiments, eco‐physiological, biochemical structural levels. The genetic basis behind these was assessed by combining selection scans differential gene expression analysis. Overall, showed reduced cuticular transpiration conjunction with consistently altered wax composition, higher accumulation two fatty alcohols iso‐alkanes. Genomic analysis unravelled nine genes associated metabolism showing allelic differentiation compared lowland populations. In silico revealed differences between ecotypes for several related metabolism. Repeated ecotypic together architecture ecotype points adaptive value colonisation habitats.

Language: Английский

---

Impact of whole-genome duplications on structural variant evolution in the plant genusCochlearia

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 30, 2023

Abstract Polyploidy, the result of whole-genome duplication (WGD), is a major driver eukaryote evolution. All angiosperms have undergone ancient WGDs, and today stable polyploids account for substantial portion both wild domesticated plant species. Despite its centrality in evolution, WGD hugely disruptive mutation, we still lack clear understanding fitness consequences. Here, study whether results greater diversity genomic structural variants (SVs) how this influences evolutionary dynamics nature. Using set long-read sequenced samples from genus Cochlearia (Brassicaceae), which contains diploids recent ploidy series up to octoploid, show that masking recessive mutations due has led progressive accumulation genic SVs across four levels. Such likely constitute genetic load thus reduce adaptive potential polyploid populations. However, SV also provides rich pool standing variation upon selection may act novel environments. By constructing graph-based pangenome , identify hundreds basis environmental adaptation. We find putatively beneficial involved pathogen resistance, root development, salt tolerance, many are unique polyploids. Finally, explore landscapes SNPs, geographical regions where make contributions variation, predict role adaptation increases rapid climate change.

Language: Английский

---

Multiple polyploidizations inArabidopsis lyratastabilized by long-range adaptive introgression across Eurasia

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 27, 2024

Abstract Abundance of polyploidy varies across lineages, evolutionary time and geography, suggesting both genetics environment play a role in polyploid persistence. Arabidopsis lyrata appears to be the most polyploidy-rich species-complex genus, with multiple origins autotetraploidy. This is revealed by genomic data from over 400 samples Eurasia. We found 30 previously undescribed autotetraploid populations Siberia minimum two separate origins, independent those reported Central Europe. The establishment Siberian tetraploids mediated meiotic adaptation at same genes as European tetraploid A. arenosa, despite high divergence geographical separation. Haplotype analysis based on synthetic long-read assemblies supports long-range introgression adaptive alleles interspecific pool arenosa . Once evolved, promotes new lineages through inter– intraspecific introgression.

Language: Английский

---