Retinal input integration in excitatory and inhibitory neurons in the mouse superior colliculus in vivo DOI Open Access
Carolin Gehr, Jérémie Sibille, Jens Kremkow

et al.

Published: Aug. 14, 2023

The superior colliculus (SC) is a midbrain structure that contains one of the highest densities inhibitory neurons in brain and, together with thalamocortical visual system, it plays key role visually guided behaviors. SC receives direct inputs from retinal ganglion cells (RGCs) but whether excitatory and differentially integrate activity vivo still largely unknown. We recently established an extracellular recording approach using high-density electrodes to measure RGCs simultaneously their postsynaptic targets , allows addressing how RGC activity. Here, we employ this method study functional properties dynamics govern retinocollicular signaling cell-type specific manner by identifying GABAergic optotagging anesthetized VGAT- ChR2 mice. measured 305 monosynaptically connected RGC-SC pairs, out which approximately third afferents connect onto neurons. show both receive comparably strong inputs, functionally similar pairs showing stronger connections. Our results demonstrate wiring rules apply for innervation neurons, unlike connectivity system. Contrasting connection strength, observed contributed more than This implies are specifically coupled afferent while may additional other sources. Taken together, our deepens understanding emphasizes two major areas processing, cortex colliculus, differently sensory inputs.

Language: Английский

Transcriptomic neuron types vary topographically in function and morphology DOI Creative Commons
Inbal Shainer, Johannes M. Kappel,

Eva Laurell

et al.

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Abstract Neuronal phenotypic traits such as morphology, connectivity and function are dictated, to a large extent, by specific combination of differentially expressed genes. Clusters neurons in transcriptomic space correspond distinct cell types some cases—for example, Caenorhabditis elegans 1 retinal ganglion cells 2–4 —have been shown share morphology function. The zebrafish optic tectum is composed spatial array that transforms visual inputs into motor outputs. Although the visuotopic map continuous, subregions functionally specialized 5,6 . Here, uncover cell-type architecture tectum, we transcriptionally profiled its neurons, revealing more than 60 organized anatomical layers. We measured responses thousands tectal two-photon calcium imaging matched them with their transcriptional profiles. Furthermore, characterized morphologies identified using transgenic lines. Notably, found similar can diverge shape, responses. Incorporating coordinates within volume revealed morphologically defined subclusters individual clusters. Our findings demonstrate extrinsic, position-dependent factors expand repertoire genetically neurons.

Language: Английский

Citations

2
Retinal input integration in excitatory and inhibitory neurons in the mouse superior colliculus in vivo DOI Creative Commons
Carolin Gehr, Jérémie Sibille, Jens Kremkow

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Sept. 8, 2023

The superior colliculus (SC) is a midbrain structure that receives inputs from retinal ganglion cells (RGCs). SC contains one of the highest densities inhibitory neurons in brain but whether excitatory and differentially integrate activity vivo still largely unknown. We recently established recording approach to measure RGCs simultaneously with their postsynaptic targets vivo, study how RGC activity. Here, we employ this method investigate functional properties govern retinocollicular signaling cell type-specific manner by identifying GABAergic using optotagging VGAT-ChR2 mice. Our results demonstrate both receive comparably strong similar wiring rules apply for innervation types, unlike connectivity thalamocortical system. Moreover, contributed more spiking compared neurons. This deepens our understanding emphasizes two major areas visual processing, cortex SC, differently sensory afferent inputs.

Language: Английский

Citations

3
Retinal input integration in excitatory and inhibitory neurons in the mouse superior colliculusin vivo DOI Creative Commons
Carolin Gehr, Jérémie Sibille, Jens Kremkow

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 8, 2023

ABSTRACT The superior colliculus (SC) is a midbrain structure that contains one of the highest densities inhibitory neurons in brain and, together with thalamocortical visual system, it plays key role visually guided behaviors. SC receives direct inputs from retinal ganglion cells (RGCs) but whether excitatory and differentially integrate activity vivo still largely unknown. We recently established an extracellular recording approach using high-density electrodes to measure RGCs simultaneously their postsynaptic targets , allows addressing how RGC activity. Here, we employ this method study functional properties dynamics govern retinocollicular signaling cell-type specific manner by identifying GABAergic optotagging anesthetized VGAT- ChR2 mice. measured 305 monosynaptically connected RGC-SC pairs, out which approximately third afferents connect onto neurons. show both receive comparably strong inputs, functionally similar pairs showing stronger connections. Our results demonstrate wiring rules apply for innervation neurons, unlike connectivity system. Contrasting connection strength, observed contributed more than This implies are specifically coupled afferent while may additional other sources. Taken together, our deepens understanding emphasizes two major areas processing, cortex colliculus, differently sensory inputs.

Language: Английский

Citations

1
Multipotent Progenitors Instruct Ontogeny of the Superior Colliculus DOI Creative Commons
Giselle Cheung, Florian M. Pauler, Peter Koppensteiner

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 16, 2023

SUMMARY The superior colliculus (SC) in the mammalian midbrain is essential for multisensory integration, attention, and complex behavior (Basso May, 2017; Cang et al., 2018). mature SC cytoarchitecture organized into distinct laminae composed of a rich variety neuronal glial cell types (Ayupe 2023; Edwards 1986; 2006; Xie 2021; Zeisel Precise execution developmental programs regulating generation cell-type diversity essential, because deficits due to genetic mutations have been associated with neurodevelopmental diseases dysfunction (Jure, 2018; McFadyen 2020). However, fundamentals directing ontogeny are not well understood. Here we pursued systematic lineage tracing at single progenitor level order decipher principles instructing SC. We combined silico reconstruction novel MADM (Mosaic Analysis Double Markers)-CloneSeq approach. MADM-CloneSeq enables unequivocal delineation lineages situ , identity based on global transcriptome, individual clonally-related cells. Our reconstructions revealed that all emerge from local progenitors without any extrinsic source. Strikingly, exceptionally multipotent capacity produce known excitatory inhibitory neuron prospective SC, clonal units showing no pre-defined composition. At molecular identified an role PTEN signaling establishing appropriate proportions specific types. Collectively, our findings demonstrate generate full spectrum developing providing framework emergence thus

Language: Английский

Citations

1
Retinal input integration in excitatory and inhibitory neurons in the mouse superior colliculus in vivo DOI Creative Commons
Carolin Gehr, Jérémie Sibille, Jens Kremkow

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: June 2, 2023

The superior colliculus (SC) is a midbrain structure that receives inputs from retinal ganglion cells (RGCs). SC contains one of the highest densities inhibitory neurons in brain but whether excitatory and differentially integrate activity vivo still largely unknown. We recently established recording approach to measure RGCs simultaneously with their postsynaptic targets vivo, study how RGC activity. Here, we employ this method investigate functional properties govern retinocollicular signaling cell type-specific manner by identifying GABAergic using optotagging VGAT-ChR2 mice. Our results demonstrate both receive comparably strong similar wiring rules apply for innervation types, unlike connectivity thalamocortical system. Moreover, contributed more spiking compared neurons. This deepens our understanding emphasizes two major areas visual processing, cortex SC, differently sensory afferent inputs.

Language: Английский

Citations

1
Retinal input integration in excitatory and inhibitory neurons in the mouse superior colliculus in vivo DOI Open Access
Carolin Gehr, Jérémie Sibille, Jens Kremkow

et al.

Published: June 2, 2023

The superior colliculus (SC) is a midbrain structure that contains one of the highest densities inhibitory neurons in brain and, together with thalamocortical visual system, it plays key role visually guided behaviors. SC receives direct inputs from retinal ganglion cells (RGCs) but whether excitatory and differentially integrate activity vivo still largely unknown. We recently established an extracellular recording approach using high-density electrodes to measure RGCs simultaneously their postsynaptic targets , allows addressing how RGC activity. Here, we employ this method study functional properties dynamics govern retinocollicular signaling cell-type specific manner by identifying GABAergic optotagging anesthetized VGAT-ChR2 mice. measured 305 monosynaptically connected RGC-SC pairs, out which approximately third afferents connect onto neurons. show both receive comparable strong inputs, functionally similar pairs showing stronger connections. Our results demonstrate wiring rules apply for innervation neurons, unlike connectivity system. Contrasting connection strength, observed contributed more than This implies are specifically coupled afferent while may additional other sources. Taken together, our deepens understanding emphasizes two major areas processing, cortex colliculus, differently sensory inputs.

Language: Английский

Citations

1
Positional information drives distinct traits in transcriptomically identified neuronal types DOI Creative Commons
Inbal Shainer, Johannes M. Kappel,

Eva Laurell

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 16, 2024

Summary Neuronal phenotypic traits such as morphology, connectivity, and function are dictated, to a large extent, by specific combination of differentially expressed genes. Clusters neurons in transcriptomic space correspond distinct cell types some cases (e. g., C. elegans 1 retinal ganglion cells 2–4 ) have been shown share morphology function. The zebrafish optic tectum is composed spatial array that transforms visual inputs into motor outputs. While the visuotopic map continuous, subregions functionally specialized 5,6 . To uncover cell-type architecture tectum, we transcriptionally profiled its neurons, revealing more than 60 organized anatomical layers. We then measured responses thousands tectal two-photon calcium imaging matched them with their transcriptional profile. Furthermore, characterized morphologies identified using transgenic lines. Surprisingly, found similar can diverge morphologically. Incorporating coordinates within volume revealed morphologically defined subclusters individual clusters. Our findings demonstrate extrinsic, position-dependent factors expand repertoire genetically neurons.

Language: Английский

Citations

0
Retinal input integration in excitatory and inhibitory neurons in the mouse superior colliculus in vivo DOI Open Access
Carolin Gehr, Jérémie Sibille, Jens Kremkow

et al.

Published: Aug. 14, 2023

The superior colliculus (SC) is a midbrain structure that contains one of the highest densities inhibitory neurons in brain and, together with thalamocortical visual system, it plays key role visually guided behaviors. SC receives direct inputs from retinal ganglion cells (RGCs) but whether excitatory and differentially integrate activity vivo still largely unknown. We recently established an extracellular recording approach using high-density electrodes to measure RGCs simultaneously their postsynaptic targets , allows addressing how RGC activity. Here, we employ this method study functional properties dynamics govern retinocollicular signaling cell-type specific manner by identifying GABAergic optotagging anesthetized VGAT- ChR2 mice. measured 305 monosynaptically connected RGC-SC pairs, out which approximately third afferents connect onto neurons. show both receive comparably strong inputs, functionally similar pairs showing stronger connections. Our results demonstrate wiring rules apply for innervation neurons, unlike connectivity system. Contrasting connection strength, observed contributed more than This implies are specifically coupled afferent while may additional other sources. Taken together, our deepens understanding emphasizes two major areas processing, cortex colliculus, differently sensory inputs.

Language: Английский

Citations

0