Mitochondrial background can explain variable costs of immune deployment DOI Creative Commons
Megan A. M. Kutzer,

Beth Cornish,

M. J. Jamieson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 6, 2023

Abstract Organismal health and survival depend on the ability to mount an effective immune response against infection. Yet, defence may be energy-demanding, resulting in fitness costs if investment function deprives other physiological processes of resources. While evidence costly immunity reduced longevity reproduction is common, role energy-producing mitochondria magnitude these unknown. Here we employed Drosophila melanogaster cybrid lines, where several mitochondrial genotypes (mitotypes) were introgressed onto a single nuclear genetic background, explicitly test variation stimulation. We exposed female flies carrying one nine distinct mitotypes either benign, heat-killed bacterial pathogen (stimulating deployment while avoiding pathology), or sterile control, measured lifespan, fecundity, locomotor activity. observed mitotype-specific stimulation identified positive correlation between lifespan proportion time cybrids spent moving alive. Our results suggests that are highly variable depending genome, adding growing body work highlighting important host-pathogen interactions.

Language: Английский

Genetic variation in trophic avoidance shows fruit flies are generally attracted to bacterial pathogens DOI Creative Commons
Katy M. Monteith, Phoebe Thornhill, Pedro F. Vale

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 10, 2024

Abstract Pathogen avoidance behaviours are often assumed to be an adaptive host defence from infection. However, there is limited experimental data on the prerequisite for this assumption: heritable, intrapopulation phenotypic variation avoidance. We investigated trophic pathogen in 122 inbred Drosophila melanogaster lines, and a derived outbred population. Using FlyPAD system, we tracked feeding choice that flies made between substrates were either clean or contained bacterial pathogen. uncovered significant, but weakly heritable preference index among fly lines. instead of avoidance, most lines demonstrated several pathogens, showing only extremely high concentrations. Bacterial was not associated with susceptibility infection retained disrupted immune signalling. Phenotype-genotype association analysis indicated novel genes ( CG2321, CG2006, ptp99A ) increased substrate, while amino-acid transporter sobremesa greater aversion. Together previous work fitness benefits consuming high-protein diets, our results suggest attraction may reflect dietary protein over carbohydrate. More quantifying needed fully assess its importance host-pathogen evolutionary ecology.

Language: Английский

Citations

2
Mitochondrial background can explain variable costs of immune deployment DOI Creative Commons
Megan A. M. Kutzer,

Beth Cornish,

M. J. Jamieson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 6, 2023

Abstract Organismal health and survival depend on the ability to mount an effective immune response against infection. Yet, defence may be energy-demanding, resulting in fitness costs if investment function deprives other physiological processes of resources. While evidence costly immunity reduced longevity reproduction is common, role energy-producing mitochondria magnitude these unknown. Here we employed Drosophila melanogaster cybrid lines, where several mitochondrial genotypes (mitotypes) were introgressed onto a single nuclear genetic background, explicitly test variation stimulation. We exposed female flies carrying one nine distinct mitotypes either benign, heat-killed bacterial pathogen (stimulating deployment while avoiding pathology), or sterile control, measured lifespan, fecundity, locomotor activity. observed mitotype-specific stimulation identified positive correlation between lifespan proportion time cybrids spent moving alive. Our results suggests that are highly variable depending genome, adding growing body work highlighting important host-pathogen interactions.

Language: Английский

Citations

0