Spatial heterogeneity of viral communities across the gastrointestinal tracts of ruminants DOI Creative Commons
Min Wang

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 11, 2024

Abstract The gastrointestinal tract (GIT) microbiome of ruminants significantly influences their nutrition metabolism and health. Current understanding is extensive for bacterial archaeal communities, but limited viral communities within the GIT. To address this, we created Ruminant Gastrointestinal Virome Catalogue (RGVC), which includes 43,981 non-redundant Operational Taxonomic Units (vOTUs), with 89.3% newly identified, derived from 370 samples across 10 GIT regions in seven ruminant species. composition mainly influenced by rather than We identified 4,603 putative prokaryotic hosts 31 three classes 5,954 viruses, along significant variations a strong correlation between associated viruses. Lysogeny, constituting 45.6% survival strategies, was more prevalent lytic cycle (4.08%), abundances these viruses varied regionally. lysogenic encoded 1,805 auxiliary metabolic genes (AMGs) that play key roles carbohydrates, amino acids, other metabolisms hosts. variation AMG abundance highlights distinct virus-host interactions Overall, this study offers comprehensive view spatial heterogeneity indicates diversity driven interaction through AMGs. These findings set stage future research into ecological nutritional impacts virome, potential to uncover novel mechanisms various regions.

Language: Английский

Spatial heterogeneity of viral communities across the gastrointestinal tracts of ruminants DOI Creative Commons
Shizhe Zhang, Qiushuang Li, Yongyi Peng

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 16, 2024

Abstract Background: The gastrointestinal tract (GIT) microbiome of ruminants significantly influences their nutrition metabolism and health. Current understanding is extensive for bacterial archaeal communities, but limited viral communities within the GIT. Results: We created Ruminant Gastrointestinal Virome Catalogue (RGVC), which includes 43,981 non-redundant Operational Taxonomic Units (vOTUs), with 89.3% newly identified, derived from 370 samples across 10 GIT regions in seven ruminant species. composition mainly influenced by rather than identified 4,603 putative prokaryotic hosts 31 three classes 5,954 viruses, along significant variations a strong correlation between associated viruses. Lysogeny, constituting 45.6% survival strategies, was more prevalent lytic cycle (4.08%), abundances these viruses varied regionally. lysogenic encoded 1,805 auxiliary metabolic genes (AMGs) that play key roles carbohydrates, amino acids, other metabolisms hosts. variation AMG abundance highlights distinct virus-host interactions Conclusion: This study offers comprehensive view spatial heterogeneity indicates this diversity driven interaction through AMGs. These findings set stage future research into ecological nutritional impacts virome, potential to uncover novel mechanisms various regions.

Language: Английский

Citations

1
A vast repertoire of secondary metabolites influences community dynamics and biogeochemical processes in cold seeps DOI Creative Commons
Xiyang Dong,

Tianxueyu Zhang,

Weichao Wu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 14, 2023

Abstract In deep sea cold seeps, diverse microbial communities thrive on the geological seepage of hydrocarbons and inorganic compounds. These chemosynthetically-driven are unique in composition, ecology, biogeochemical activities compared to photosynthetically-driven ecosystems. However, their biosynthetic capabilities remain largely unexplored. Here, we analyzed 81 metagenomes, 33 metatranscriptomes, seven metabolomes derived from nine globally distributed areas seeps investigate secondary metabolites produced by seep microbiomes. Cold microbiomes encode diverse, abundant, novel gene clusters (BGCs). Most BGCs affiliated with understudied bacteria archaea, including key mediators methane sulfur cycling, multiple candidate phyla. The antimicrobial compounds (e.g. NRPS, PKSs, RiPPs) that potentially shape community dynamics, as well predicted influence such phosphonates, iron-acquiring siderophores, nitrogenase-protecting glycolipids, methyl-CoM reductase-modifying proteins. players widely highly expressed, abundance expression levels varying different sediment depths. Numerous natural products were detected through untargeted metabolomics, demonstrating a vast, unexplored chemical space validating situ sediments. Overall, these results demonstrate sediments serve reservoir hidden provide insights into adaptation

Language: Английский

Citations

2
Spatial heterogeneity of viral communities across the gastrointestinal tracts of ruminants DOI Creative Commons
Min Wang

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 11, 2024

Abstract The gastrointestinal tract (GIT) microbiome of ruminants significantly influences their nutrition metabolism and health. Current understanding is extensive for bacterial archaeal communities, but limited viral communities within the GIT. To address this, we created Ruminant Gastrointestinal Virome Catalogue (RGVC), which includes 43,981 non-redundant Operational Taxonomic Units (vOTUs), with 89.3% newly identified, derived from 370 samples across 10 GIT regions in seven ruminant species. composition mainly influenced by rather than We identified 4,603 putative prokaryotic hosts 31 three classes 5,954 viruses, along significant variations a strong correlation between associated viruses. Lysogeny, constituting 45.6% survival strategies, was more prevalent lytic cycle (4.08%), abundances these viruses varied regionally. lysogenic encoded 1,805 auxiliary metabolic genes (AMGs) that play key roles carbohydrates, amino acids, other metabolisms hosts. variation AMG abundance highlights distinct virus-host interactions Overall, this study offers comprehensive view spatial heterogeneity indicates diversity driven interaction through AMGs. These findings set stage future research into ecological nutritional impacts virome, potential to uncover novel mechanisms various regions.

Language: Английский

Citations

0