Coxsackievirus A24 variant associated with acute hemorrhagic conjunctivitis outbreak in Bhutan, 2023 DOI Creative Commons
Sonam Gyeltshen, Tshering Dorji, Kunzang Dorji

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 28, 2025

Abstract In 2023, Bhutan detected an outbreak of acute hemorrhagic conjunctivitis (AHC) in southern and central regions, coinciding with similar outbreaks reported South Southeast Asia. Laboratory results from clinical specimens were initially inconclusive identifying the etiological agent. To address this, 18 samples, comprising conjunctival swabs throat/nasal nine patients collected sent to WRAIR-AFRIMS for further analysis. Specimens tested using multiplex real-time RT-PCR (Fast-track respiratory 21 kit, FTD21) hybrid-capture-based next-generation sequencing (NGS) Illumina Viral Surveillance Panel. FTD21 testing identified human adenovirus, bocavirus, influenza A, enterovirus, and/or rhinovirus 10/18 (56%). A higher detection rate was observed (78%, 7/9) compared (33%, 3/9) same patients, highlighting increased sensitivity samples causative agents conjunctivitis. Further assessment by NGS only coxsackievirus A24 variant (CVA24v) genotype IV 9/17 (53%), primarily samples. Phylogenetic analyses CVA24v VP1 sequences revealed genetic distinctions 2023 isolates prior 2002–2017, suggesting re-emergence driven novel mutations. These findings suggest that are more reliable detecting agent AHC swabs. Additionally, identification a strain underscores importance genomics.

Language: Английский

Coxsackievirus A24 variant associated with acute hemorrhagic conjunctivitis outbreak in Bhutan, 2023 DOI Creative Commons
Sonam Gyeltshen, Tshering Dorji, Kunzang Dorji

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 28, 2025

Abstract In 2023, Bhutan detected an outbreak of acute hemorrhagic conjunctivitis (AHC) in southern and central regions, coinciding with similar outbreaks reported South Southeast Asia. Laboratory results from clinical specimens were initially inconclusive identifying the etiological agent. To address this, 18 samples, comprising conjunctival swabs throat/nasal nine patients collected sent to WRAIR-AFRIMS for further analysis. Specimens tested using multiplex real-time RT-PCR (Fast-track respiratory 21 kit, FTD21) hybrid-capture-based next-generation sequencing (NGS) Illumina Viral Surveillance Panel. FTD21 testing identified human adenovirus, bocavirus, influenza A, enterovirus, and/or rhinovirus 10/18 (56%). A higher detection rate was observed (78%, 7/9) compared (33%, 3/9) same patients, highlighting increased sensitivity samples causative agents conjunctivitis. Further assessment by NGS only coxsackievirus A24 variant (CVA24v) genotype IV 9/17 (53%), primarily samples. Phylogenetic analyses CVA24v VP1 sequences revealed genetic distinctions 2023 isolates prior 2002–2017, suggesting re-emergence driven novel mutations. These findings suggest that are more reliable detecting agent AHC swabs. Additionally, identification a strain underscores importance genomics.

Language: Английский

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