Cell cycle controls long-range calcium signaling in the regenerating epidermis

The Journal of Cell Biology, Journal Year: 2023, Volume and Issue: 222(7)

Published: April 27, 2023

Skin homeostasis is maintained by stem cells, which must communicate to balance their regenerative behaviors. Yet, how adult cells signal across tissue remains unknown due challenges in studying signaling dynamics live mice. We combined imaging the mouse basal cell layer with machine learning tools analyze patterns of Ca2+ signaling. show that display dynamic intercellular among local neighborhoods. find these signals are coordinated thousands and this coordination an emergent property layer. demonstrate G2 required initiate normal levels signaling, while connexin43 connects orchestrate tissue-wide Lastly, we drives cycle progression, revealing a communication feedback loop. This work provides resolution into at different stages coordinate during epidermal regeneration.

Language: Английский

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Calling long distance: Cell cycle–dependent Ca2+ flows connect stem cells across regeneration tissues

The Journal of Cell Biology, Journal Year: 2023, Volume and Issue: 222(7)

Published: June 14, 2023

How adult stem cells signal in vivo over time to coordinate their fate and behavior across self-renewing tissues remains a challenging question. In this issue, Moore et al. (2023. J. Cell Biol.https://doi.org/10.1083/jcb.202302095) combine high-resolution live imaging mice with machine learning tools reveal temporally regulated tissue-scale patterns of Ca2+ signaling orchestrated by cycling basal the skin epidermis.

Language: Английский

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The PHATE of basal epidermal cells: Calcium signaling during the cell cycle

Cell Calcium, Journal Year: 2023, Volume and Issue: 114, P. 102768 - 102768

Published: June 15, 2023

Language: Английский

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