Impact of inter-species interactions between flower microbiota commensals and a floral pathogen on disease incidence and pathogen activity DOI Creative Commons
M. Amine Hassani, Zhouqi Cui, Jacquelyn LaReau

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 16, 2023

Abstract Flowers are colonized by a diverse community of microorganisms that alter plant health and interact with floral pathogens. Erwinia amylovora is flower-inhabiting bacterium pathogen infects different species, including Malus × domestica (apple). Previously, we showed the co-inoculation two bacterial strains, Pseudomonas Pantoea natural inhabitants apple flowers, reduced disease incidence caused this pathogen. Here, decipher ecological interactions between these flower-associated bacteria E. in field experimentation vitro co-cultures. The flower microbiota strains did not competitively exclude from stigma habitat, as both commensal co-existed on proscribing microbial antibiosis or niche exclusion predominant mechanisms host protection. Inspection total active populations flowers revealed strain co-inoculations altered activity. Using synthetic exudation medium, tripartite co-culture strongly acidified growth milieu led to substantial alteration gene expression members. Our study emphasizes critical role emergent properties mediated inter-species within holobiont their impact behavior. Importance Fire blight, , one most important diseases pome fruits. Plant-associated can influence occurrence through interactions. Previous studies have shown commensals could suppress mainly antagonizing growth, however, whether plant-associated activity behavior been well studied. show naturally colonize reduce pressure. We further demonstrate lead strong pH emergence new patterns may findings emphasize pathogens health.

Language: Английский

Ancient DNA genomics and the renaissance of herbaria DOI
Hernán A. Burbano, Rafał M. Gutaker

Science, Journal Year: 2023, Volume and Issue: 382(6666), P. 59 - 63

Published: Oct. 6, 2023

Herbaria are undergoing a renaissance as valuable sources of genomic data for exploring plant evolution, ecology, and diversity. Ancient DNA retrieved from herbarium specimens can provide unprecedented glimpses into past communities, their interactions with biotic abiotic factors, the genetic changes that have occurred over time. Here, we highlight recent advances in field genomics discuss challenges opportunities combining modern time-stamped historical specimens. We also describe how integrating other types yield substantial insights evolutionary ecological processes shape communities. Herbarium analysis is tool understanding life informing conservation efforts face dire environmental challenges.

Language: Английский

Citations

21
Novel phages of Pseudomonas syringae unveil numerous potential auxiliary metabolic genes DOI
Chloé Feltin, Julian R. Garneau, Cindy E. Morris

et al.

Journal of General Virology, Journal Year: 2024, Volume and Issue: 105(6)

Published: June 4, 2024

Relatively few phages that infect plant pathogens have been isolated and investigated. The Pseudomonas syringae species complex is present in various environments, including plants. It can cause major crop diseases, such as bacterial canker on apricot trees. This study presents a collection of 25 unique phage genomes P. . These were from orchards with symptoms after enrichment 21 strains comprises mostly virulent phages, only three being temperate. They belong to 14 genera, 11 which are newly discovered, 18 new species, revealing great genetic diversity within this collection. Novel DNA packaging systems identified bioinformatically one the but experimental confirmation required define precise mechanism. Additionally, many contain numerous potential auxiliary metabolic genes diversified putative functions. At least encode involved tellurite resistance, toxic metalloid. suggests viruses could play role stress tolerance. research emphasizes significance continuing search for agricultural ecosystem unravel novel ecological gene work contributes foundation future fundamental applied infecting phytopathogenic bacteria.

Language: Английский

Citations

1
Evidence for the key roles of thePseudomonas syringaemobilome in shaping biotic interactions DOI Open Access
Dominique Holtappels,

G.E.J. Rickus,

Túlio Morgan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 20, 2024

Abstract The mobilome, defined as the collection of mobile genetic elements within a bacterial genome, plays critical role in adaptation bacteria to abiotic and biotic drivers. In particular, prophages have been reported contribute resistance virulent bacteriophages, competitive interaction hosts microbial communities, pathogenicity virulence. It is therefore better understand distributing genes functions among species predict how adapt their environment. Pseudomonas syringae offers an ideal study system ask these questions both because its broad range lifestyles (spanning from environmental growth plant pathogens) high intraspecies diversity. To examine mobilome this complex, we compared 590 genomes available public databases annotated defense mechanisms, effectors, genomes. We found that complex has elaborate phage pandefensome consisting 139 mechanisms. Host-associated P. isolates were defensomes effectoromes. Assessing taxonomical signatures observed uncovered differences types numbers encoded by different families, emphasizing evolutionary advantages conferred will depend on prophage composition offering insight might disperse community. Our highlights intimate association families with uncovers key shaping ecology for widespread complex. Significance statement accessory including prophages, These are across taxa likely maintained advantage. ability population evolve over time requires understanding where functional traits arrive. address question, assessed prophage-encoded defenses effector . show carrying belong specific encoded. This emphasizes advantage framework uncover communities pathogen evolution.

Language: Английский

Citations

0
Novel phages of Pseudomonas syringae unveil numerous potential auxiliary metabolic genes DOI Creative Commons
Chloé Feltin, Julian R. Garneau, Cindy E. Morris

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 7, 2024

Abstract Relatively few phages that infect plant pathogens have been isolated and investigated. The Pseudomonas syringae species complex is present in various environments, including plants. It can cause major crop diseases, such as bacterial canker on apricot trees. This study presents a collection of 25 unique genomes P. . These were from orchards with symptoms after enrichment 21 strains comprises mostly virulent phages, only three being temperate. They belong to 14 genera, 11 which are newly discovered, 18 new species, revealing great genetic diversity within this collection. Novel DNA packaging systems identified bioinformatically one the phage but experimental confirmation required define precise mechanism. Additionally, many contain numerous potential auxiliary metabolic genes diversified putative functions. At least encode involved tellurite resistance, toxic metalloid. suggests viruses could play role stress tolerance. research emphasises significance continuing search for agricultural ecosystem unravel novel ecological gene work contributes foundation future fundamental applied infecting phytopathogenic bacteria.

Language: Английский

Citations

0
Impact of inter-species interactions between flower microbiota commensals and a floral pathogen on disease incidence and pathogen activity DOI Creative Commons
M. Amine Hassani, Zhouqi Cui, Jacquelyn LaReau

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 16, 2023

Abstract Flowers are colonized by a diverse community of microorganisms that alter plant health and interact with floral pathogens. Erwinia amylovora is flower-inhabiting bacterium pathogen infects different species, including Malus × domestica (apple). Previously, we showed the co-inoculation two bacterial strains, Pseudomonas Pantoea natural inhabitants apple flowers, reduced disease incidence caused this pathogen. Here, decipher ecological interactions between these flower-associated bacteria E. in field experimentation vitro co-cultures. The flower microbiota strains did not competitively exclude from stigma habitat, as both commensal co-existed on proscribing microbial antibiosis or niche exclusion predominant mechanisms host protection. Inspection total active populations flowers revealed strain co-inoculations altered activity. Using synthetic exudation medium, tripartite co-culture strongly acidified growth milieu led to substantial alteration gene expression members. Our study emphasizes critical role emergent properties mediated inter-species within holobiont their impact behavior. Importance Fire blight, , one most important diseases pome fruits. Plant-associated can influence occurrence through interactions. Previous studies have shown commensals could suppress mainly antagonizing growth, however, whether plant-associated activity behavior been well studied. show naturally colonize reduce pressure. We further demonstrate lead strong pH emergence new patterns may findings emphasize pathogens health.

Language: Английский

Citations

0