Chromosome‐level assembly of basil genome unveils the genetic variation driving Genovese and Thai aroma types DOI
Jing Zhang,

Mohamad Abu‐Abied,

Renana Milavski

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(2)

Published: Jan. 1, 2025

Basil, Ocimum basilicum L., is a widely cultivated aromatic herb, prized for its culinary and medicinal uses, predominantly owing to unique aroma, primarily determined by eugenol Genovese cultivars or methyl chavicol Thai cultivars. To date, comprehensive basil reference genome has been lacking, with only fragmented draft available. fill this gap, we employed PacBio HiFi Hi-C sequencing construct homeolog-phased chromosome-level basil. The tetraploid was assembled into 26 pseudomolecules further categorized subgenomes. High levels of synteny were observed between the two subgenomes but comparisons Salvia rosmarinus show collinearity quickly breaks down in near relatives. We utilized bi-parental population derived from × cross map quantitative trait loci (QTL) aroma chemotype. discovered single QTL governing eugenol/methyl ratio, which encompassed genomic region 95 genes, including 15 genes encoding shikimate O-hydroxycinnamoyltransferase (HCT/CST) enzyme. Of them, ObHCT1 exhibited significantly higher expression cultivar showed trichome-specific expression. functionally confirmed as genuine HCT enzyme using an vitro assay. high-quality, contiguous now publicly accessible at BasilBase, valuable resource scientific community. Combined insights cell-type-specific gene expression, it promises elucidate specialized metabolite biosynthesis pathways cellular level.

Language: Английский

Chromosome‐level assembly of basil genome unveils the genetic variation driving Genovese and Thai aroma types DOI
Jing Zhang,

Mohamad Abu‐Abied,

Renana Milavski

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(2)

Published: Jan. 1, 2025

Basil, Ocimum basilicum L., is a widely cultivated aromatic herb, prized for its culinary and medicinal uses, predominantly owing to unique aroma, primarily determined by eugenol Genovese cultivars or methyl chavicol Thai cultivars. To date, comprehensive basil reference genome has been lacking, with only fragmented draft available. fill this gap, we employed PacBio HiFi Hi-C sequencing construct homeolog-phased chromosome-level basil. The tetraploid was assembled into 26 pseudomolecules further categorized subgenomes. High levels of synteny were observed between the two subgenomes but comparisons Salvia rosmarinus show collinearity quickly breaks down in near relatives. We utilized bi-parental population derived from × cross map quantitative trait loci (QTL) aroma chemotype. discovered single QTL governing eugenol/methyl ratio, which encompassed genomic region 95 genes, including 15 genes encoding shikimate O-hydroxycinnamoyltransferase (HCT/CST) enzyme. Of them, ObHCT1 exhibited significantly higher expression cultivar showed trichome-specific expression. functionally confirmed as genuine HCT enzyme using an vitro assay. high-quality, contiguous now publicly accessible at BasilBase, valuable resource scientific community. Combined insights cell-type-specific gene expression, it promises elucidate specialized metabolite biosynthesis pathways cellular level.

Language: Английский

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