GENOMIC DIVERSITY OF TOXIGENIC VIBRIO CHOLERAE O1 BIOVAR EL TOR STRAINS ISOLATED DURING THREE WAVES OF THE 7TH CHOLERA PANDEMIC DOI Creative Commons
N. I. Smirnova,

Д. А. Рыбальченко,

Yulii Vladislavovich Lozovskiy

et al.

Russian Journal of Infection and Immunity, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 16, 2024

Introduction. High genome variability of the 7th cholera pandemic agent, V. cholerae El Tor, led to emergence genovariants with distinct set altered genes. The aim work was analyze dynamics changes in pathogenicity, epidemicity as well drug resistance and phylogeny toxigenic strains Tor isolated Russia endemic regions during three waves ongoing pandemic. Materials methods. We used whole-genome nucleotide sequences 155 strains, obtained by us (42) taken from NCBI Genbank (113). DNA sequencing performed on Ion PGM platform. Phylogenetic relations were determined based Bayesian analysis core SNPs using Snippy 4.6 software package. Antibiotic assessed applying disk diffusion test. Results. SNP data revealed that studied (1970-2023) might be divided into clusters. A clear correlation between each-cluster strain genotype relevant isolation timing observed. Separation genetically cluster II III 2nd 3rd typical I is associated acquisition new mutations pathogenicity Due different combination mutations, are heterogeneous. Genome comparison showed this diversity increased dramatically wave, which higher pathogenic epidemic potential. It demonstrated antibiotic both over past 30 years (1993-2023) has undergone significant changes. Thereat, changing clearly correlated occurrence various Conclusion. shown two decades agent underwent a rather rapid change resulting genovariants. pathogen variants been established. Strains combining genetic markers hyper-virulence multiple particular concern. identified points at need for constant genomic surveillance obtain altering epidemically important properties timely generation diagnostic preventive means.

Language: Английский

New Vibrio cholerae sequences from Eastern and Southern Africa alter our understanding of regional cholera transmission DOI Creative Commons
Shaoming Xiao,

Ahmed Abade,

Waqo Boru

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 30, 2024

Despite ongoing containment and vaccination efforts, cholera remains prevalent in many countries sub-Saharan Africa. Part of the difficulty containing comes from our lack understanding how it circulates throughout region. To better characterize regional transmission, we generated analyzed 118

Language: Английский

Citations

3
Genomic epidemiology of the cholera outbreak in Malawi 2022-2023 DOI Creative Commons

Lucious Chabuka,

Wonderful T. Choga, Carla Mavian

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 24, 2023

Abstract Since early 2022, in the aftermath of two extreme weather events, Malawi experienced its largest ever cholera outbreak, with over 58,000 reported cases and 1,761 deaths as May 2023. We generated 49 high-quality, near-complete Vibrio cholerae genomes from isolates collected between December 2022 March 2023 all three regions Malawi. Using phylogenetic methods 2159 publicly available genomes, we present evidence suggesting that outbreak strains originated Pakistan estimated most recent ancestor this lineage, named T15, was during floods, once introduced into exacerbated by major floods June October 2022. The events humanitarian crises provided environment for spread cholerae, subsequent movement large numbers people may have facilitated to susceptible populations areas relatively unaffected a decade.

Language: Английский

Citations

8
Multicountry genomic analysis underscores regional cholera spread in Africa DOI Creative Commons
Gerald Mboowa,

Nathaniel Lucero Matteson,

Collins Kipngetich Tanui

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

ABSTRACT Cholera remains a significant public health burden in many countries sub-Saharan Africa, though the exact mechanisms of bacterial emergence and spread remain largely undefined. We generated genomic data from 728 Vibrio cholerae O1 isolates predominantly collected between 2019-2024 to create largest dataset V. genomes sequenced locally Africa. This enabled us interrogate recent patterns spread, including rapid circulation AFR15 lineage associated with unusually large outbreaks Southern provide evidence for movement into new African Member States confirm previously observed differences transmission dynamics West versus East cross-border is prevalent on both sides continent. Despite differences, evolutionary processes are similar across lineages we find no changes antimicrobial resistance genotypes. Overall, our findings emphasize importance regionally coordinated surveillance interventions, while also demonstrating critical role understanding cholera

Language: Английский

Citations

2
Cholera Outbreaks in Zimbabwe: An In‐Depth Analysis of Drivers, Constraints and Reimagining the Use of Medicinal Plants DOI Creative Commons
Jerikias Marumure, Monde A. Nyila

Journal of Tropical Medicine, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

Cholera, an intestinal infection caused by

Language: Английский

Citations

0
GENOMIC DIVERSITY OF TOXIGENIC VIBRIO CHOLERAE O1 BIOVAR EL TOR STRAINS ISOLATED DURING THREE WAVES OF THE 7TH CHOLERA PANDEMIC DOI Creative Commons
N. I. Smirnova,

Д. А. Рыбальченко,

Yulii Vladislavovich Lozovskiy

et al.

Russian Journal of Infection and Immunity, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 16, 2024

Introduction. High genome variability of the 7th cholera pandemic agent, V. cholerae El Tor, led to emergence genovariants with distinct set altered genes. The aim work was analyze dynamics changes in pathogenicity, epidemicity as well drug resistance and phylogeny toxigenic strains Tor isolated Russia endemic regions during three waves ongoing pandemic. Materials methods. We used whole-genome nucleotide sequences 155 strains, obtained by us (42) taken from NCBI Genbank (113). DNA sequencing performed on Ion PGM platform. Phylogenetic relations were determined based Bayesian analysis core SNPs using Snippy 4.6 software package. Antibiotic assessed applying disk diffusion test. Results. SNP data revealed that studied (1970-2023) might be divided into clusters. A clear correlation between each-cluster strain genotype relevant isolation timing observed. Separation genetically cluster II III 2nd 3rd typical I is associated acquisition new mutations pathogenicity Due different combination mutations, are heterogeneous. Genome comparison showed this diversity increased dramatically wave, which higher pathogenic epidemic potential. It demonstrated antibiotic both over past 30 years (1993-2023) has undergone significant changes. Thereat, changing clearly correlated occurrence various Conclusion. shown two decades agent underwent a rather rapid change resulting genovariants. pathogen variants been established. Strains combining genetic markers hyper-virulence multiple particular concern. identified points at need for constant genomic surveillance obtain altering epidemically important properties timely generation diagnostic preventive means.

Language: Английский

Citations

0