Dynamic evolution of a sex-linked region DOI Creative Commons
Xiaomeng Mao, Nima Rafati, Christian Tellgren‐Roth

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Abstract Background Sex chromosomes often evolve exceptionally fast and degenerate after recombination arrest. However, the underlying evolutionary processes are under persistent debate, particularly whether or not arrest evolves in a stepwise manner how switches sex determination genes contribute to chromosome evolution. Here, we study dioecious plant genus Salix with high turnover of chromosomes. Results We identified Z W sex-linked regions (~ 8 Mb) on 15 dwarf willow Salix herbacea using new haplotype-resolved assembly. The region harboured large (5 embedded inversion. Analyses synteny other species, sequence divergence between degeneration suggest that inversion recently incorporated pseudoautosomal sequences into region, extending its length nearly three-fold. W-hemizygous exclusively contained seven pairs inverted partial repeats male essential floral identity gene PISTILLATA, suggesting possible PISTILLATA suppression mechanism by interfering RNA females. Such pseudogenes were also found species ZW but those XY determination. Conclusions Our provides rare compelling direct support for long-standing theory reduction mediated inversions suggests Salicaceae family is associated switch gene.

Language: Английский

Chromosome-level genome assembly of black carp Mylopharyngodon piceus using Nanopore and Hi-C technologies DOI Creative Commons
Yuxuan Zhang, Yanwen Shao, Xudong Liu

et al.

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: Jan. 25, 2025

Language: Английский

Citations

0
Insights into adhesive and neuronal cell populations of the chaetognath Spadella cephaloptera using a single-nuclei transcriptomic atlas and genomic resources DOI Open Access

Cristian Camilo Barrera Grijalba,

June F. Ordoñez, Juan D. Montenegro

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Abstract To cope with extreme environmental conditions diverse marine species have developed mechanisms that allow them to permanently or temporarily attach substrates. In the intertidal zone of habitats, where tidal ranges and currents may drift organisms away from their habitat, temporary adhesive systems such as one inherent arrow worm Spadella cephaloptera (Chaetognatha) constitute an essential trait for survival this taxon. The underlying molecular mechanism system has not been described yet, existing morphological information is limited adults. Furthermore, a relationship between nervous attachment in S. remains be demonstrated. study, single-nuclei sequencing hatchlings was performed, using reference newly sequenced assembled genome identify transcriptomic profiles cells mediating attachment, neuronal populations, main cell types chaetognath hatchlings. Our findings, supported by previous studies, suggest evolved convergently those other metazoans. Moreover, were identified ventral nerve center multiple ciliated previously anatomical observations validated. Ongoing in-depth investigation these data, together datasets developmental stages, will provide further insights into evolutionary origins unique body plan.

Language: Английский

Citations

0
A CACTA-like transposon in the Anthocyanidin synthase 1 (Ans-1) gene is responsible for apricot fruit colour in the raspberry (Rubus idaeus) cultivar ‘Varnes’ DOI Creative Commons
Daniel James Sargent, Matteo Buti, Stefan Martens

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0318692 - e0318692

Published: Feb. 3, 2025

Cultivated raspberries ( Rubus idaeus L.) most commonly bear small, red, highly aromatic fruits. Their colour is derived predominantly from anthocyanins, water soluble polyphenolic pigments, but as well red forms, there exist cultivars that display yellow- and apricot-coloured In this investigation, we used a multi-omics approach to elucidate the genetic basis of apricot fruit in raspberry. Using metabolomics, quantified anthocyanins raspberry fruits demonstrated that, contrast red-fruited raspberries, cultivar ‘Varnes’ contain low concentrations only small number anthocyanin compounds. By performing RNASeq, revealed differential expression patterns apricot-fruited for genes biosynthesis pathway following whole genome sequencing using long-read Oxford Nanopore Technologies sequencing, identified CACTA-like transposable element (TE) second exon Anthocyanidin synthase Ans ) gene caused truncated predicted ANS protein. PCR confirmed presence heterozygous form transposon an unrelated, ‘Veten’, indicating recessive it may be widespread germplasm, potentially explaining why forms appear at regular intervals modern breeding populations.

Language: Английский

Citations

0
Chromosomal genome assembly resolves drug resistance loci in the parasitic nematode Teladorsagia circumcincta DOI Creative Commons
Jennifer McIntyre, Alison Morrison, Kirsty Maitland

et al.

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(2), P. e1012820 - e1012820

Published: Feb. 6, 2025

The parasitic nematode Teladorsagia circumcincta is one of the most important pathogens sheep and goats in temperate climates worldwide can rapidly evolve resistance to drugs used control it. To understand genetics drug resistance, we have generated a highly contiguous genome assembly for UK T. isolate, MTci2. Assembly using PacBio long-reads Hi-C long-molecule scaffolding together with manual curation resulted 573 Mb (N50 = 84 Mb, total scaffolds 1,286) five autosomal sex-linked chromosomal-scale consistent its karyotype. resource was further improved via annotation 22,948 genes, over 3,200 these, resulting robust near complete (96.3% protein BUSCOs) support basic applied research on this veterinary pathogen. Genome-wide analyses combining evidence from three distinct experiments, identified selection around known candidate genes benzimidazole, levamisole ivermectin as well novel regions associated moxidectin resistance. These insights into contemporary historic genetic emphasise importance assemblies interpreting genome-wide variation identifying key loci prioritise developing diagnostic markers anthelmintic parasite control.

Language: Английский

Citations

0
Dynamic evolution of a sex-linked region DOI Creative Commons
Xiaomeng Mao, Nima Rafati, Christian Tellgren‐Roth

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Abstract Background Sex chromosomes often evolve exceptionally fast and degenerate after recombination arrest. However, the underlying evolutionary processes are under persistent debate, particularly whether or not arrest evolves in a stepwise manner how switches sex determination genes contribute to chromosome evolution. Here, we study dioecious plant genus Salix with high turnover of chromosomes. Results We identified Z W sex-linked regions (~ 8 Mb) on 15 dwarf willow Salix herbacea using new haplotype-resolved assembly. The region harboured large (5 embedded inversion. Analyses synteny other species, sequence divergence between degeneration suggest that inversion recently incorporated pseudoautosomal sequences into region, extending its length nearly three-fold. W-hemizygous exclusively contained seven pairs inverted partial repeats male essential floral identity gene PISTILLATA, suggesting possible PISTILLATA suppression mechanism by interfering RNA females. Such pseudogenes were also found species ZW but those XY determination. Conclusions Our provides rare compelling direct support for long-standing theory reduction mediated inversions suggests Salicaceae family is associated switch gene.

Language: Английский

Citations

0