Cooperative role of LSD1 and CHD7 in regulating differentiation of mouse embryonic stem cells DOI Creative Commons
Sandhya Malla, Carlos Martinez‐Gamero, Kanchan Kumari

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 18, 2024

Abstract Lysine-specific histone demethylase 1 (LSD1) is a that plays critical role in epigenetic regulation by removing the methyl group from mono- and di-methylated lysine 4 on H3 (H3K4me1/2), acting as repressor of gene expression. Recently, catalytically independent functions LSD1, serving scaffold for assembling chromatin-regulator transcription factor complexes, have been identified. Herein, we show first time LSD1 interacts with chromodomain-helicase-DNA-binding protein 7 (CHD7) mouse embryonic stem cells (ESCs). To further investigate CHD7–LSD1 crosstalk , engineered Chd7 Chd7/Lsd1 knockout (KO) ESCs. We CHD7 dispensable ESC self-renewal survival, while KO ESCs can differentiate towards embryoid bodies (EBs) defective expression ectodermal markers. Intriguingly, double exhibit proliferation defects similar to Lsd1 lost capacity properly. Furthermore, increased co-occupancy H3K4me1 chromatin following deletion suggests required facilitating proper binding regulating differentiation. Collectively, our results suggest work concert modulate influence cell fate determination.

Language: Английский

Cooperative role of LSD1 and CHD7 in regulating differentiation of mouse embryonic stem cells DOI Creative Commons
Sandhya Malla, Carlos Martinez‐Gamero, Kanchan Kumari

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 18, 2024

Abstract Lysine-specific histone demethylase 1 (LSD1) is a that plays critical role in epigenetic regulation by removing the methyl group from mono- and di-methylated lysine 4 on H3 (H3K4me1/2), acting as repressor of gene expression. Recently, catalytically independent functions LSD1, serving scaffold for assembling chromatin-regulator transcription factor complexes, have been identified. Herein, we show first time LSD1 interacts with chromodomain-helicase-DNA-binding protein 7 (CHD7) mouse embryonic stem cells (ESCs). To further investigate CHD7–LSD1 crosstalk , engineered Chd7 Chd7/Lsd1 knockout (KO) ESCs. We CHD7 dispensable ESC self-renewal survival, while KO ESCs can differentiate towards embryoid bodies (EBs) defective expression ectodermal markers. Intriguingly, double exhibit proliferation defects similar to Lsd1 lost capacity properly. Furthermore, increased co-occupancy H3K4me1 chromatin following deletion suggests required facilitating proper binding regulating differentiation. Collectively, our results suggest work concert modulate influence cell fate determination.

Language: Английский

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